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avrilcoghlan/run_genewisedb_afterblast.pl

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Perl script to run GeneWise by comparing a file of multiple of HMMs to a fasta file of multiple sequences, by running GeneWise on the regions of the DNA sequences where the proteins used to make the HMM have tblastn matches
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run_genewisedb_afterblast.pl
#!/usr/local/bin/perl
=head1 NAME
run_genewisedb_afterblast.pl
=head1 SYNOPSIS
run_genewisedb_afterblast.pl input_fasta input_hmms output outputdir spliceflat parameterfile treefam_seqs eval_cutoff flank_length
where input_fasta is the input fasta file of scaffolds,
input_hmms is the input file of HMMs,
output is the genewise output file,
outputdir is the output directory for writing output files,
spliceflat says whether to use the -splice flat option (yes/no),
parameterfile is the name of the intron parameter file (none if there is none),
treefam_seqs is the file of protein sequences in TreeFam families,
eval_cutoff is the evalue cutoff to use for blast matches,
flank_length is the length of DNA to take on either side of a blast match.
=head1 DESCRIPTION
This script takes an input fasta file of scaffolds (<input_fasta>) and an input
file of HMMs (<input_hmms>). For each HMM and scaffold, we first check is there
a blast match between the HMM and scaffold. If so, we run genewise on the scaffold,
using that HMM. The output is then written in the output file <output>.
Before running this perl script you need to run:
setenv WISECONFIGDIR /nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/wisecfg/
=head1 VERSION
Perl script last edited 8-Nov-2012.
=head1 CONTACT
[email protected] (Avril Coghlan)
=cut
#
# Perl script run_genewisedb_afterblast.pl
# Written by Avril Coghlan ([email protected])
# 8-Nov-12.
# Last edited 8-Nov-2012.
#
#------------------------------------------------------------------#
# CHECK IF THERE ARE THE CORRECT NUMBER OF COMMAND-LINE ARGUMENTS:
use strict;
use warnings;
my $num_args = $#ARGV + 1;
if ($num_args != 9)
{
print "Usage of run_genewisedb_afterblast.pl\n\n";
print "perl run_genewisedb_afterblast.pl <input_fasta> <input_hmms> <output> <outputdir> <spliceflat> <parameterfile> <treefam_seqs> <eval_cutoff> <flank_length>\n";
print "where <input_fasta> is the input fasta file,\n";
print " <input_hmms> is the input file of HMMs,\n";
print " <output> is the genewise output file,\n";
print " <outputdir> is the output directory for writing output files,\n";
print " <spliceflat> says whether to use the -splice flat option (yes/no),\n";
print " <parameterfile> is the name of the intron parameter file (none if there is none),\n";
print " <treefam_seqs> is the file of protein sequences in TreeFam families,\n";
print " <eval_cutoff> is the evalue cutoff to use for blast matches,\n";
print " <flank_length> is the length of DNA to take on either side of a blast match\n";
print "For example, >perl run_genewisedb_afterblast.pl \n";
print "/nfs/helminths02/analysis/50HGP/Brugia.pahangi/ASSEMBLY/v1.0.pipeline/S07.Im.SS.Gf.Rr.scaffolds.fa\n";
print "/lustre/scratch108/parasites/es9/GeneWise/TreeFam8.hmm genewise_output\n";
print "/nfs/users/nfs_a/alc/Documents/GeneWise50Helminths no mygenestat.stat\n";
print "treefam8_seqs 0.05 25000\n";
print "NOTE: Before running this perl script, you need to run:\n";
print "setenv WISECONFIGDIR /nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/wisecfg/\n";
exit;
}
# FIND THE PATH TO THE INPUT FASTA FILE:
my $input_fasta = $ARGV[0];
# FIND THE INPUT FILE OF HMMs:
my $input_hmms = $ARGV[1];
# FIND THE GENEWISE OUTPUT FILE:
my $output = $ARGV[2];
# FIND THE DIRECTORY TO USE FOR OUTPUT FILES:
my $outputdir = $ARGV[3];
# FIND OUT WHETHER WE WANT TO USE THE -splice flat OPTION IN GENEWISE:
my $spliceflat = $ARGV[4];
# FIND THE NAME OF THE INTRON PARAMETER FILE:
my $parameterfile = $ARGV[5];
# FIND THE NAME OF THE FILE WITH PROTEIN SEQUENCES IN TREEFAM FAMILIES:
my $treefam_seqs = $ARGV[6];
# FIND THE EVALUE CUTOFF TO USE FOR BLAST MATCHES:
my $evalue_cutoff = $ARGV[7];
# FIND THE LENGTH OF DNA TO TAKE ON EITHER SIDE OF A BLAST MATCH:
my $flank_length = $ARGV[8];
#------------------------------------------------------------------#
# CHECK THAT THE WISECONFIGDIR IS SET CORRECTLY:
(my $errorcode,my $errormsg) = &check_wiseconfigdir;
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
#------------------------------------------------------------------#
# TEST SUBROUTINES:
my $PRINT_TEST_DATA = 0; # SAYS WHETHER TO PRINT DATA USED DURING TESTING.
&test_print_error;
&test_write_fasta_file($outputdir);
&test_get_seqs_in_family($outputdir);
&test_make_hmmfile($outputdir);
&test_make_seqfile($outputdir);
&test_convert_to_scaffold_coordinates($outputdir);
&test_merge_overlapping_hits;
&test_check_for_blast_match2($outputdir);
&test_run_genewise_on_scaffolds2($outputdir);
#------------------------------------------------------------------#
# RUN THE MAIN PART OF THE CODE:
&run_main_program($outputdir,$input_fasta,$input_hmms,$output,$spliceflat,$parameterfile,$treefam_seqs,$evalue_cutoff,$flank_length);
print STDERR "FINISHED.\n";
#------------------------------------------------------------------#
# RUN THE MAIN PART OF THE CODE:
sub run_main_program
{
my $outputdir = $_[0]; # DIRECTORY TO PUT OUTPUT FILES IN.
my $input_fasta = $_[1]; # THE INPUT FASTA FILE
my $input_hmms = $_[2]; # THE INPUT FILE OF HMMs
my $output = $_[3]; # THE OUTPUT FILE NAME
my $spliceflat = $_[4]; # SAYS WHETHER WE WANT TO USE THE -splice flat OPTION IN GENEWISE
my $parameterfile = $_[5]; # THE NAME OF THE INTRON PARAMETER FILE
my $treefam_seqs = $_[6]; # FILE OF PROTEIN SEQUENCES IN TREEFAM FAMILIES
my $evalue_cutoff = $_[7]; # EVALUE CUTOFF TO USE FOR BLAST MATCHES
my $flank_length = $_[8]; # LENGTH OF SEQUENCE TO TAKE ON EITHER SIDE OF A BLAST MATCH
my $errorcode; # RETURNED AS 0 IF THERE IS NO ERROR.
my $errormsg; # RETURNED AS 'none' IF THERE IS NO ERROR.
# RUN GENEWISE WITH ONE SCAFFOLD/CONTIG AT A TIME, ON ONE HMM AT A TIME:
($errorcode,$errormsg) = &run_genewise_on_scaffolds2($outputdir,$input_fasta,$input_hmms,$output,$spliceflat,$parameterfile,$treefam_seqs,$evalue_cutoff,$flank_length,0);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
}
#------------------------------------------------------------------#
# TEST &run_genewise_on_scaffolds2
sub test_run_genewise_on_scaffolds2
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES TO
my $input_fasta; # INPUT FASTA FILE
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
my $input_hmms; # INPUT FILE OF HMMs
my $fam_seqs; # INPUT FILE WITH SEQUENCES IN FAMILIES.
my $output; # OUTPUT FILE FOR GENEWISE OUTPUT
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $expected_output; # FILE CONTAINING THE EXPECTED CONTENT OF $output
my $differences; # DIFFERENCES BETWEEN $output AND $expected_output
my $length_differences; # LENGTH OF $differences
my $line; #
# DEFINE THE OUTPUT FILE NAME FOR GENEWISE OUTPUT:
$random_number = rand();
$output = "tmp".$random_number;
# WRITE A FILE WITH THE INPUT SEQUENCES IN THE FAMILY:
$random_number = rand();
$fam_seqs = $outputdir."/tmp".$random_number;
open(FAM_SEQS,">$fam_seqs") || die "ERROR: test_run_genewise_on_scaffolds2: cannot open $fam_seqs\n";
print FAM_SEQS "TF101001\n";
print FAM_SEQS ">At1g16330.1\n";
print FAM_SEQS "MLRDGNKQSKNNVRFVRKSIKTTVKTSLQNRSSLKKPPVGRSKSRSISSIPSSAVASTLSLPEKVETKCLEEDTQGESSSSGNKDPTTKVLDVTAKPKSKRRKSFTSLLVNGSKFDEKNGETTEPEKLPSIDDESNQLEVAEYVDDIYQFYWTAEALNPALGHYLSAHAEVSPVTRGILINWLIEVHFKFDLMHETLYLTMDLLDRYLSQVPIHKNEMQLIGLTALLLASKYEDYWHPRIKDLISISAESYT\n";
print FAM_SEQS "REQILGMERSMLKQLKFRLNAPTPYVFMLRFLKAAQSNKKLEQLAFYLIELCLVEYEALKYKPSLLCASAIYVARCTLHMTPVWTSLLNNHTHYNVSQMKDCSDMILRFHKAAKTGNLRVTYEKYINPDRSNVAVLKPLDKLPL\n";
print FAM_SEQS ">At1g20590.1\n";
print FAM_SEQS "MSFLAVHQIVRKKLQLVGVTALLLACKYEEVSVPVVDDLILISDKAYSRREVLDMEKLMANTLQFNFSLPTPYVFMKRFLKAAQSDKKLEILSFFMIELCLVEYEMLEYLPSKLAASAIYTAQCTLKGFEEWSKTCEFHTGYNEEQLLACARKMVAFHHKAGTGKLTGSTTHLSSFMLQEVNQLGFCFKGGKNYNKNLI\n";
print FAM_SEQS ">At1g20610.1\n";
print FAM_SEQS "MVRSDENSLGLIGSMSLQGGGVVGKIKTTATTGPTRRALSTINKNITEAPSYPYAVNKRSVSERDGICNKPPVHRPVTRKFAAQLADHKPHIRDEETKKPDSVSSEEPETIIIDVDESDKEGGDSNEPMFVQHTEAMLEEIEQMEKEIEMEDADKEEEPVIDIDACDKNNPLAAVEYIHDMHTFYKNFEKLSCVPPNYMDNQQDLNERMRGILIDWLIEVHYKFELMEETLYLTINVIDRFLAVHQIVRKKL\n";
print FAM_SEQS "QLVGVTALLLACKYEEVSVPVVDDLILISDKAYSRREVLDMEKLMANTLQFNFSLPTPYVFMKRFLKAAQSDKKLEILSFFMIELCLVEYEMLEYLPSKLAASAIYTAQCTLKGFEEWSKTCEFHTGYNEKQLLACARKMVAFHHKAGTGKLTGVHRKYNTSKFCHAARTEPAGFLI\n";
print FAM_SEQS ">At1g34460.1\n";
print FAM_SEQS "MAHYINIYGKRLFDIGLYLKTSVDLILQQQHVLMSQQKFTDISLCHREAPNPKLDLPDDNRLSPLTELEDIGQQSKTLGTAIHRKISEQKRVIESGSVASIVEHKRTNLSAIMATRANIPEQVRGAPLVDGLKIQNKNGAVKNRRALGDIGNLVSVPGVQGGKAQPPINRPITLSFRAQLLANAQLERKPINGDNKVPALGPKSQPLAARNPEAQRAVQKKNLVVKQQ\n";
print FAM_SEQS "TKPVEVIETKRNAQSKAACGIVNK\n";
print FAM_SEQS "PKILDIDESDKDNHVAAVEYVDDMYSFYKEVEKESQPKMYMHIQTEMNEKMRAILIDWLLEVHIKFELNLETLYLTVNIIDRFLYVKAVPKRELQVNDLVYVTDNAYSSRQILVMKKAILGNLEWYLTIPTQYVFLFCFIKASISDPEVLHVQKKNLQASKTKSFSIQVLSFSSHKSIVKSDQFCKKFNLCQEVTAPASEFHLGNCEAWRETVTKLKDPETKGSLVSRDFSSCKELLIKGFKMKEC\n";
print FAM_SEQS "LAAKKI\n";
print FAM_SEQS ">At1g47210.2\n";
print FAM_SEQS "MTEQEICVRVTRAAAKRKASTAMGIDGDRVNKKRVVLGELLNVSNVNLLANLNQKKETQKPKRNLKPPPAKQIKSAPVAIIDLESKSDIDSRSDDPQMCGPYVADIYEYLRQLEVKPKQRPLPDYIEKVQKDVTPSMRGVLVDWLVEVAEEYKLGSETLYLTVSHIDRFLSLKTVNKQKLQLVGVSAMLIASKYEEISPPKVDDFCYITDNTFSKQDVVKMEADILLALQFELGRPTINTFMRRFTRVAQDD\n";
print FAM_SEQS "FKVPHLQLEPLCCYLSELSILDYKTVKFVPSLLAASAVFLARFIIRPKQHPWNQMLEEYTKYKAADLQVCVGIIHDLYLSRRGGALQAVREKYKHHKFQCVATMPVSPELPVTFWEDVTI\n";
print FAM_SEQS ">At1g47220.1\n";
print FAM_SEQS "MVSKKDKQIHSDHNPQDASPVEWAEIRHKLLAFQENIQSGSDIDARSDDPQMCGLYVSDIYEYLRELEVKPKLRPLHDYIEKIQEDITPSKRGVLVDWLVEVAEEFELVSETLYLTVSYIDRFLSLKMVNEHWLQLVGVSAMFIASKYEEKRRPKVEDFCYITANTYTKQDVLKMEEDILLALEFELGRPTTNTFLRRFIRVAQEDFKVPNLQLEPLCCYLSELSMLDYSCVKFVPSLLAASAVFLARFIIL\n";
print FAM_SEQS "PNQHPWSQMLEECTKYKAADLQVCVEIMLDLYLSRSEGASKAVREKYKQHKFQYVAAIPVYQELPVTFWEDVVTI\n";
print FAM_SEQS ">At1g76310.1\n";
print FAM_SEQS "MGGSDENRHGVIGPMNRQQGGLRGGKVIPTNGQTRRALSNINKNIIGAPVYPCAVKRPFTEKNGICNKKIPPVPVHRPVTRKFAAQLAENNLQIHKEETKKPDLISNEALDRIITDVEEGDFNEPMFVQHTEAMLEEIDKMEGIEMQDSNDIDAEVEESVMDIDSCDKNNPLSVVEYINDIYCFYKKNECRSCVPPNYMENQHDINERMRGILFDWLIEVHYKFELMEETLYLTINLIDRFLAVHQHIARKK\n";
print FAM_SEQS "LQLVGVTAMLLACKYEEVSVPVVDDLILISDKAYTRTEILDMEKLMANTLQFNFCLPTPYVFMRRFLKAAQSDKKLELLSFFMIELCLVEYEMLQYTPSQLAASAIYTAQSTLKGYEDWSKTSEFHSGYTEEALLECSRKMVGLHHKAGTGKLTGVHRKYNTSKFGYAARIEPAGFLLL\n";
print FAM_SEQS ">At2g17620.1\n";
print FAM_SEQS "MVNSCENKIFVKPTSTTILQDETRSRKFGQEMKREKRRVLRVINQNLAGARVYPCVVNKRGSLLSNKQEEEEGCQKKKFDSLRPSVTRSGVEEETNKKLKPSVPSANDFGDCIFIDEEEATLDLPMPMSLEKPYIEADPMEEVEMEDVTVEEPIVDIDVLDSKNSLAAVEYVQDLYAFYRTMERFSCVPVDYMMQQIDLNEKMRAILIDWLIEVHDKFDLINETLFLTVNLIDRFLSKQNVMRKKLQLVGLV\n";
print FAM_SEQS "ALLLACKYEEVSVPVVEDLVLISDKAYTRNDVLEMEKTMLSTLQFNISLPTQYPFLKRFLKAAQADKKCEVLASFLIELALVEYEMLRFPPSLLAATSVYTAQCTLDGSRKWNSTCEFHCHYSEDQLMECSRKLVSLHQRAATGNLTGVYRKYSTSKFGYIAKCEAAHFLVSESHHS\n";
print FAM_SEQS ">At2g26760.1\n";
print FAM_SEQS "MASSRVSDLPHQRGIAGEIKPKNVAGHGRQNRKVLGDIGNLVTGRDVATGKDVAKKAKQPQQQTKAEVIVISPDENEKCKPHFSRRTHIRGTKTFTATLRARSKAASGLKDAVIDIDAVDANNELAAVEYVEDIFKFYRTVEEEGGIKDYIGSQPEINEKMRSILIDWLVDVHRKFELMPETLYLTINLVDRFLSLTMVHRRELQLLGLGAMLIACKYEEIWAPEVNDFVCISDNAYNRKQVLAMEKSILGQ\n";
print FAM_SEQS "VEWYITVPTPYVFLARYVKAAVPCDAEMEKLVFYLAELGLMQYPIVVLNRPSMLAASAVYAARQILKKTPFWTETLKHHTGYSEDEIMEHAKMLMKLRDSASESKLIAVFKKYSVSENAEVALLPSLDDFSVSCA\n";
print FAM_SEQS ">At3g11520.1\n";
print FAM_SEQS "MATGPVVHPQPVRGDPIDLKNAAAKNRRALGDIGNVDSLIGVEGGKLNRPITRNFRAQLLENAQVAAAANKKAPILDGVTKKQEVVRAVQKKARGDKREPSKPIEVIVISPDTNEVAKAKENKKKVTYSSVLDARSKAASKTLDIDYVDKENDLAAVEYVEDMYIFYKEVVNESKPQMYMHTQPEIDEKMRSILIDWLVEVHVKFDLSPETLYLTVNIIDRFLSLKTVPRRELQLVGVSALLIASKYEEIWP\n";
print FAM_SEQS "PQVNDLVYVTDNSYNSRQILVMEKTILGNLEWYLTVPTQYVFLVRFIKASGSDQKLENLVHFLAELGLMHHDSLMFCPSMLAASAVYTARCCLNKTPTWTDTLKFHTGYSESQLMDCSKLLAFIHSKAGESKLRGVLKKYSKLGRGAVALISPAKSLMSSAP\n";
print FAM_SEQS ">At4g35620.1\n";
print FAM_SEQS "MVNPEENNRNLVVKPITEILQDDDKRSRKFGVEMKRQNRRALGVINHNLVGAKAYPCVVNKRRGLSQRKQESCDKKKLDSLHPSISRSQEETKKLKPSGNEFGDCIFIDEEEEKNEEVTLDQPMPMSLEEPYIEFDPMEEEVEMEDMEEEQEEPVLDIDEYDANNSLAAVEYVQDLYDFYRKTERFSCVPLDYMAQQFDISDKMRAILIDWLIEVHDKFELMNETLFLTVNLIDRFLSKQAVARKKLQLVGL\n";
print FAM_SEQS "VALLLACKYEEVSVPIVEDLVVISDKAYTRTDVLEMEKIMLSTLQFNMSLPTQYPFLKRFLKAAQSDKKLEILASFLIELALVDYEMVRYPPSLLAATAVYTAQCTIHGFSEWNSTCEFHCHYSENQLLECCRRMVRLHQKAGTDKLTGVHRKYSSSKFGYIATKYEAAHFLVSDSH\n";
print FAM_SEQS ">At4g37490.1\n";
print FAM_SEQS "MMTSRSIVPQQSTDDVVVVDGKNVAKGRNRQVLGDIGNVVRGNYPKNNEPEKINHRPRTRSQNPTLLVEDNLKKPVVKRNAVPKPKKVAGKPKVVDVIEISSDSDEELGLVAAREKKATKKKATTYTSVLTARSKAACGLEKKQKEKIVDIDSADVENDLAAVEYVEDIYSFYKSVESEWRPRDYMASQPDINEKMRLILVEWLIDVHVRFELNPETFYLTVNILDRFLSVKPVPRKELQLVGLSALLMSAK\n";
print FAM_SEQS "YEEIWPPQVEDLVDIADHAYSHKQILVMEKTILSTLEWYLTVPTHYVFLARFIKASIADEKMENMVHYLAELGVMHYDTMIMFSPSMVAASAIYAARSSLRQVPIWTSTLKHHTGYSETQLMDCAKLLAYQQWKQQEEGSESSTKGALRKKYSKDERFAVALIPPAKALLTGTESA\n";
print FAM_SEQS ">At5g06150.1\n";
print FAM_SEQS "MATRANVPEQVRGAPLVDGLKIQNKNGAVKSRRALGDIGNLVSVPGVQGGKAQPPINRPITRSFRAQLLANAQLERKPINGDNKVPALGPKRQPLAARNPEAQRAVQKKNLVVKQQTKPVEVIETKKEVTKKEVAMSPKNKKVTYSSVLSARSKAACGIVNKPKIIDIDESDKDNHLAAVEYVDDMYSFYKEVEKESQPKMYMHIQTEMNEKMRAILIDWLLEVHIKFELNLETLYLTVNIIDRFLSVKAVP\n";
print FAM_SEQS "KRELQLVGISALLIASKYEEIWPPQVNDLVYVTDNAYSSRQILVMEKAILGNLEWYLTVPTQYVFLVRFIKASMSDPEMENMVHFLAELGMMHYDTLTFCPSMLAASAVYTARCSLNKSPAWTDTLQFHTGYTESEIMDCSKLLAFLHSRCGESRLRAVYKKYSKAENGGVAMVSPAKSLLSAAADWKKPVSS\n";
print FAM_SEQS ">At5g11300.1\n";
print FAM_SEQS "MYCSSSMHPNANKENISTSDVQESFVRITRSRAKKAMGRGVSIPPTKPSFKQQKRRAVLKDVSNTSADIIYSELRKGGNIKANRKCLKEPKKAAKEGANSAMDILVDMHTEKSKLAEDLSKIRMAEAQDVSLSNFKDEEITEQQEDGSGVMELLQVVDIDSNVEDPQCCSLYAADIYDNIHVAELQQRPLANYMELVQRDIDPDMRKILIDWLVEVSDDYKLVPDTLYLTVNLIDRFLSNSYIERQRLQLLG\n";
print FAM_SEQS "VSCMLIASKYEELSAPGVEEFCFITANTYTRPEVLSMEIQILNFVHFRLSVPTTKTFLRRFIKAAQASYKVPFIELEYLANYLAELTLVEYSFLRFLPSLIAASAVFLARWTLDQTDHPWNPTLQHYTRYEVAELKNTVLAMEDLQLNTSGCTLAATREKYNQPKFKSVAKLTSPKRVTSLFSR\n";
print FAM_SEQS ">At5g25380.1\n";
print FAM_SEQS "MHRASSKHTNAKKEAISTSKIRDNNVRVTRSRAKALGVSNSPSKPAFKHETKRVARPSNKRMASDNITVCNQKRRAVLKDVTNTLAESIISTEGNVKACKRGGKETKQIEEDGLVDVDGEKSKLAEDLSKIRMVESLDASASKQKEDRSDVTDCVQIVDIDSGVQDPQFCSLYAASIYDSINVAELEQRPSTSYMVQVQRDIDPTMRGILIDWLVEVSEEYKLVSDTLYLTVNLIDRFMSHNYIEKQKLQLL\n";
print FAM_SEQS "GITCMLIASKYEEISAPRLEEFCFITDNTYTRLEVLSMEIKVLNSLHFRLSVPTTKTFLRRFIRAAQASDKVPLIEMEYLANYFAELTLTEYTFLRFLPSLIAASAVFLARWTLDQSNHPWNQTLQHYTRYETSALKNTVLAMEELQLNTSGSTLIAIHTKYNQQKFKRVATLTSPERVNTLFSR\n";
print FAM_SEQS ">At5g43080.1\n";
print FAM_SEQS "MADEKENCVRMTRAATKRKASMEAAIDKERINKKRVVLGELPNLSNIKKSRKATTKQKKKSVSIPTIETLNSDIDTRSDDPQMCGPYVTSIFEYLRQLEVKSRPLVDYIEKIQKDVTSNMRGVLVDWLVEVAEEYKLLSDTLYLAVSYIDRFLSLKTVNKQRLQLLGVTSMLIASKYEEITPPNVDDFCYITDNTYTKQEIVKMEADILLALQFELGNPTSNTFLRRFTRVAQEDFEMSHLQMEFLCSYLSE\n";
print FAM_SEQS "LSMLDYQSVKFLPSTVAASAVFLARFIIRPKQHPWNVMLEEYTRYKAGDLKECVAMIHDLYLSRKCGALEAIREKYKQHKFKCVATMPVSPELPLTVFEDVNI\n";
print FAM_SEQS ">CBG05553\n";
print FAM_SEQS "MMMLRSQAKNVDPPADGRQHQKRKQAEQLDALKNPLEPVAKKQLSKGLTELRGHLSGFKIDSAKREQQGGKKNPSNNDDSENIAPGKAPYVDPCPHYDYDQEEAHNPDSVSEYAPEIFDYYKAREIQFQVLPYLARHPEIDVKTRAILIDWMVEIQETFELNHETLYNAVKLTDIFLHNSKNVDKNTIQKLACVTIFIAAKYDERSPPLVDDLIYLSGDRFTREELLAMERSLFSTVGYDLGSPLSYRYLRR\n";
print FAM_SEQS "LGRVCRVDMRSLTMARYILETSLMVYDYAFISQARQAAAAFVLAMRMMAKDHRYEWNPVLEKYSGFTGEEIYPLVEHMNHIMHFVTAKWTQLASVRQKYSHEVFFHVAEIPFLPDTLKAVNAHTYAPVPVFSYP\n";
print FAM_SEQS "#END\n";
close(FAM_SEQS);
# WRITE AN INPUT FASTA FILE:
$random_number = rand();
$input_fasta = $outputdir."/tmp".$random_number;
open(INPUT_FASTA,">$input_fasta") || die "ERROR: test_run_genewise_on_scaffolds2: cannot open $input_fasta\n";
print INPUT_FASTA ">V:15395346..15397430\n";
print INPUT_FASTA "gaatgttattttcttatcaataagcacattttctatcaaacccagttcactttcaaattt\n";
print INPUT_FASTA "ttcaatcacttttttctgaacttttaagcatttttgtcgacagtgagaaatatcgccgac\n";
print INPUT_FASTA "cgtactccgaggcatgtgcgctccaccgaacggggggcaatttcaaatacaccccgcgag\n";
print INPUT_FASTA "acccaccaagagggcggcaatttccagtgttcaccatcgtttgccatttgctctctgctc\n";
print INPUT_FASTA "ccaaaaatcatcatttttttggtttcatcgcttcgcagtttccgtttaatattttgtctc\n";
print INPUT_FASTA "cattttccccactttcaatgtaatttatttcagtattttcagcattcaaaatgatgctgc\n";
print INPUT_FASTA "gaagccaggccaagaatgtcgacctgacttcacaaggtgagcggatttcctcgaaaacta\n";
print INPUT_FASTA "tttatagattttaaattttaatttacagcagattctcggcatcaacaaaaacgaaagcag\n";
print INPUT_FASTA "gctgagcaattggatgctctaaagaatccatcggaaccggcagccaagaagcaacacagc\n";
print INPUT_FASTA "aaggtagaacgaggattatcgattttttgggccttctcaaattaattgtttcagggtctc\n";
print INPUT_FASTA "accgagctccgcgcacacatctctggatttaaaatcgactcggcaaagcgagatccactg\n";
print INPUT_FASTA "ggaaagtcgagaacgagtagacgagacgtcgagaaccttccaccgcaaaagtccagatat\n";
print INPUT_FASTA "gttgatccgtgtcctcattacgattacgacctcgaggaggccggaaacccggacagtatc\n";
print INPUT_FASTA "tcggactatgctcaagggatttttgactactacagacaccgagaagttcactttcgagtg\n";
print INPUT_FASTA "agaaagtaccttcacaaacatccggaggttgacgtcaagactcgtgctatcctcattgac\n";
print INPUT_FASTA "tggatggttgagattcaggagacatttgagctcaaccacgagaccctttacaacgccgtt\n";
print INPUT_FASTA "aagctcacggatatgtacttgtgcaagacgaagaatgttgacaaaaacaccattcaggtg\n";
print INPUT_FASTA "agataaaatccaaagtagtctattatttttatcaataaattttcagaagcttgcatgtgt\n";
print INPUT_FASTA "cgccatcttcattgccgccaagtacgatgagcgatctccaccactcgtcgatgatctcat\n";
print INPUT_FASTA "ctacctttccggagatcgcttctcccgcgatgagcttcttgccatggagcgtgaactctt\n";
print INPUT_FASTA "cgccaccgtcggatatgatctcggctcaccactgagctatcggtatcttcgccgcttcgg\n";
print INPUT_FASTA "tcgtgtctgtcgtgtcgatatgaagacactcaccatgggacgcttcattctggagacatc\n";
print INPUT_FASTA "actgatggtctacgaatacgctatggtctcacaatcccgtcttgccgccgccgctttcgt\n";
print INPUT_FASTA "cttggccatgcgtatgctcgacaagaataatgagtacgagtggaatccagtgctcgagaa\n";
print INPUT_FASTA "gtattctggattcactggagaggaggtgatgccactggtggagcacatgaaccacattct\n";
print INPUT_FASTA "tcatttttcgaaggacaagtgggctcaactcacatccgttcgccagaagtattctcatga\n";
print INPUT_FASTA "gtgagtttttgcaaagaaatatcgatttttaatatttttttccagagtattcttccatgt\n";
print INPUT_FASTA "cgcctcgatcccaatgctcccggataccctcaaggttgtggattcccacacatatgctcc\n";
print INPUT_FASTA "agtcccaatgctctcatacccataactaatcctaatttattcccgtattggattctgaat\n";
print INPUT_FASTA "cccctctcaactgttcacccaccaaaactttcaaacgattgacacctatgcctcttttga\n";
print INPUT_FASTA "tatattttgtatacgtattgcaattctcttctgtattccatttaactcctgtcatctctc\n";
print INPUT_FASTA "acaaatacacatacgcaattttggcctgattttgttttttgcttttaaaaatctgaacat\n";
print INPUT_FASTA "attgcaaaatttctaccaatttttaaacacatttcgagctacgccaaaataaattatttt\n";
print INPUT_FASTA "tccggcaaattgtttattgatggtgtgatttcagattaatgactaatgtggagacttgaa\n";
print INPUT_FASTA "cttgatcggcgcaactttacaagcggtttttgtccttacagtgct\n";
close(INPUT_FASTA);
# WRITE AN INPUT FILE OF HMMs:
$random_number = rand();
$input_hmms = $outputdir."/tmp".$random_number;
open(INPUT_HMMS,">$input_hmms") || die "ERROR: test_run_genewise_on_scaffolds2: cannot open $input_hmms\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101001\n";
print INPUT_HMMS "LENG 442\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "RF no\n";
print INPUT_HMMS "CS no\n";
print INPUT_HMMS "MAP yes\n";
print INPUT_HMMS "COM /software/ensembl/compara/hmmer-2.3.2/src/hmmbuild --amino /tmp/treefam/TF101001/hmmer.hmm /tmp/treefam/TF101001/TF101001.sto\n";
print INPUT_HMMS "NSEQ 41\n";
print INPUT_HMMS "DATE Wed Sep 12 16:30:05 2012\n";
print INPUT_HMMS "CKSUM 9556\n";
print INPUT_HMMS "XT -8455 -4 -1000 -1000 -8455 -4 -8455 -4 \n";
print INPUT_HMMS "NULT -4 -8455\n";
print INPUT_HMMS "NULE 595 -1558 85 338 -294 453 -1158 197 249 902 -1085 -142 -21 -313 45 531 201 384 -1998 -644 \n";
print INPUT_HMMS "HMM A C D E F G H I K L M N P Q R S T V W Y \n";
print INPUT_HMMS " m->m m->i m->d i->m i->i d->m d->d b->m m->e\n";
print INPUT_HMMS " -55 * -4742\n";
print INPUT_HMMS " 1 -411 970 176 -169 -2686 -3421 -2158 1227 25 12 1788 -634 -75 -1970 951 -2393 502 605 683 -2581 86\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9846 -10888 -894 -1115 -701 -1378 -55 * \n";
print INPUT_HMMS " 2 661 -3551 -1928 -525 -3871 -1382 649 -3621 219 -182 -2640 1302 -3147 737 427 1355 1064 -3172 -3734 -81 87\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9846 -10888 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 3 56 -3547 -1969 596 -3853 -3087 -1747 -946 -267 -941 -152 530 598 -92 1803 1201 560 -1070 -3738 -3064 88\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9877 -10919 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 4 -652 248 -2002 483 -3912 799 1730 -3657 -1367 -1603 1703 1895 -3217 638 -392 504 925 -3217 -3788 -3110 89\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9926 -10969 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 5 -426 -3582 -501 -600 -3886 -1353 459 439 1009 -236 1354 1538 -795 502 -1880 409 161 -594 -3775 -55 90\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9926 -10969 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 6 -2261 -2850 -2680 -660 502 -1112 -16 1171 908 -1326 493 -691 -970 -1907 1938 446 -2201 714 -3212 -85 91\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 7 -38 -3625 -2014 -431 -3942 -30 -1798 226 572 -2021 -2715 809 -811 1199 1733 701 -599 214 -3811 -3131 92\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 8 -753 -3448 679 -1566 -3689 -733 378 1633 -1475 -1083 274 1297 -729 1415 -825 348 265 -147 -3681 -3050 93\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 9 1267 -3484 -508 863 -913 -1830 -1844 -1024 -1454 -781 756 -267 1444 627 87 -953 127 289 -3707 -3067 94\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 10 -1207 631 31 1780 -3950 -1130 -1796 -3699 1189 -921 -79 1723 -3230 -54 -1884 798 -666 -572 -3815 -3134 95\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 11 -1168 -3635 0 1058 -3957 -1035 266 -3707 14 -954 -2725 2843 -811 857 -296 -250 86 -3258 -3819 -3136 96\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 12 -305 -3633 452 288 -3953 -3137 1206 -233 322 -387 -2722 1524 347 558 -657 232 77 505 -3817 -3135 97\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -24 -9947 -6003 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 13 493 649 996 386 -3929 -1350 -1778 287 -340 -2268 -2701 819 -696 1420 166 -850 -51 957 -3796 -3115 98\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -25 -9925 -5981 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 14 -797 -3421 -2073 -474 -609 503 -1817 -876 1124 -246 -2530 130 1088 572 -1925 1027 272 180 -3651 -3018 99\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 15 -266 -3599 999 311 -3919 1107 -1759 -768 1398 -3615 -2688 1069 -3193 679 -1846 380 561 -1102 -3782 -3099 100\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 16 -2138 -3469 -2044 109 -3734 493 1736 413 1266 -1583 1048 435 595 33 -1902 -124 -355 142 -3687 706 101\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -40 -9903 -5267 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 17 512 -2322 -3689 -104 -2300 -3701 -2512 1670 -959 248 -164 -443 -3762 -154 -2991 -1010 -2227 2329 -2758 -2383 102\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9865 -10908 -894 -1115 -1722 -521 * * \n";
print INPUT_HMMS " 18 823 -3566 216 192 -3887 1011 -1728 -3637 -274 -179 -213 1443 -1585 1052 -590 -246 -173 -1385 -3750 -8 103\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -99 -9865 -3937 -894 -1115 -1722 -521 * * \n";
print INPUT_HMMS " 19 527 -3489 858 -320 -3810 1305 663 -1065 923 -1481 -2579 1074 -3084 636 588 -275 -1956 -814 -3673 -2990 104\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9769 -10811 -894 -1115 -2369 -310 * * \n";
print INPUT_HMMS " 20 652 -3464 591 319 -416 -1224 563 -1154 2223 -1418 -2557 -185 -3090 -1201 -1748 974 -1958 -510 -3655 -2979 105\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9769 -10811 -894 -1115 -2369 -310 * * \n";
print INPUT_HMMS " 21 -1077 -3492 -597 -463 -3813 783 631 -3564 2084 -1426 -2581 1918 -587 701 1152 -1899 -1958 -3114 -3675 -2992 106\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9769 -10811 -894 -1115 -2369 -310 * * \n";
print INPUT_HMMS " 22 733 545 -2069 -1518 -3347 -1357 2024 -3011 291 -3116 -2283 -1797 818 -1368 359 411 1614 401 -3426 828 107\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -50 -9769 -4931 -894 -1115 -2369 -310 * * \n";
print INPUT_HMMS " 23 725 -3403 -1852 -233 -3701 -2966 466 497 197 -1331 -436 -43 -982 1984 722 554 -656 582 -3599 -2930 108\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -59 -9722 -4677 -894 -1115 -2593 -261 * * \n";
print INPUT_HMMS " 24 564 -3029 -2018 -1466 -3204 -893 783 -1104 866 428 -2159 -1739 788 -423 -420 993 712 515 -3307 -2727 109\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -115 -9665 -3726 -894 -1115 -2815 -221 * * \n";
print INPUT_HMMS " 25 -1853 -3289 -1714 -1165 -3596 -8 1424 -97 1149 -3299 -2382 -328 -2924 258 1278 854 1775 -293 -3481 -2808 110\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -230 -9553 -2774 -894 -1115 -3156 -172 * * \n";
print INPUT_HMMS " 26 12 -3143 -1519 90 -3464 -989 -1304 -713 1791 -3159 -2232 1308 1140 481 -25 823 99 -2765 -3327 -2644 111\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -35 -9326 -5474 -894 -1115 -3620 -122 * * \n";
print INPUT_HMMS " 27 144 -2356 -112 -1546 1234 -86 -1605 -144 -46 486 -1518 -1748 -263 -1339 -97 1622 -702 -666 -2711 -2226 112\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -161 -9294 -3263 -894 -1115 -3669 -118 * * \n";
print INPUT_HMMS " 28 -277 -2934 -1413 212 -3224 641 -1184 -2953 240 -1363 951 1100 -306 -734 -1279 1975 27 -1135 -3136 -2472 113\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -127 -9136 -3601 -894 -1115 -3880 -101 * * \n";
print INPUT_HMMS " 29 253 -2226 -1796 -1240 -2317 -2640 1147 778 -1126 -1173 -1382 370 -228 341 -1544 1958 919 -347 -2567 -2062 114\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -45 -9014 -5133 -894 -1115 -4013 -92 * * \n";
print INPUT_HMMS " 30 -1417 -2884 -1264 673 -3202 -239 -1050 -262 406 -2898 -1974 1224 17 599 -1139 1765 1105 -2504 -3069 -2387 115\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -8973 -10015 -894 -1115 -4052 -90 * * \n";
print INPUT_HMMS " 31 -118 -1719 -2582 -2009 478 -581 -1663 -1273 470 1081 1743 -2029 -103 -1661 974 207 167 241 -2138 -1739 116\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -8973 -10015 -894 -1115 -4052 -90 * * \n";
print INPUT_HMMS " 32 -81 -2884 -1262 343 -3203 -533 -1047 -2953 1441 -821 -1973 421 -793 2172 407 -50 164 -297 -3068 -2386 117\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -99 -8973 -3952 -894 -1115 -4052 -90 * * \n";
print INPUT_HMMS " 33 513 -2814 -1199 908 -3131 1218 -984 -156 -566 -2828 -1904 1306 580 836 800 -25 -1290 -2434 -3000 -2319 118\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -341 -8878 -2264 -894 -1115 -4135 -85 * * \n";
print INPUT_HMMS " 34 1296 -1851 -1518 -962 -1931 422 1520 383 510 -828 -1011 692 -2412 -761 -1251 27 -1121 973 -2200 -1706 119\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -61 -8544 -4690 -894 -1115 -4363 -72 * * \n";
print INPUT_HMMS " 35 -1202 -2360 -1198 -633 -2584 -2217 -861 -2252 1325 81 -1481 -908 1698 1067 656 275 -1135 935 -2594 -2005 120\n";
print INPUT_HMMS " - -146 -507 230 41 -384 398 99 -627 210 -466 -727 275 392 52 97 363 119 -362 -301 -256 \n";
print INPUT_HMMS " - -950 -1056 -9531 -3332 -151 -44 -5070 * * \n";
print INPUT_HMMS " 36 -792 -3632 -2011 280 -3952 -395 1292 -1182 2273 -3648 17 -247 -15 1668 764 -2043 -975 -168 -3816 -3134 142\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 37 -1031 -3653 -2030 158 -3977 -3155 66 -3726 2113 -3668 -2743 1484 1764 -1350 1567 -602 -178 -3277 -3833 -3154 143\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 38 -4933 -5397 -6207 -4292 -354 -5364 -3082 -5461 724 -1299 -4469 -4045 -5251 -2697 3955 -4815 -4533 -5349 -4799 -4552 144\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 39 503 -3625 -2016 -1467 -3941 -3140 1201 -3688 1075 -3639 -626 380 -1164 -55 1841 730 1173 346 -3811 -3132 145\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 40 2598 -2588 -3805 -3253 -2686 -1355 -2770 -649 -3044 -2567 -1891 -1297 -1198 -216 -3203 -1619 -714 2112 -3127 -2747 146\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 41 -864 -2444 -4869 -4252 2041 -4143 -3019 -1827 -701 2669 -1539 -856 -4190 -3484 -3655 -3237 -2553 -103 -2881 -2533 147\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 42 -2246 -3721 300 1452 -4041 2544 -1871 -3793 -53 -3737 -2815 -1826 -3299 -1414 -693 838 -603 -1182 -3906 -3219 148\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 43 -2540 -4040 3030 1760 -4344 -3371 -2135 -4105 -1784 -4049 -3144 1085 -948 26 -2316 -2399 -943 -566 1371 -3515 149\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 44 -3862 -3461 -6392 -5932 -3731 -5948 -5228 3035 -5696 811 -2622 -760 -5820 -1424 -5641 -5167 -3832 2185 -4882 -4509 150\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -100 -9947 -3924 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 45 -2183 -3560 -2041 -1525 -3912 1912 -191 -3644 -444 -2152 -2719 1753 337 -1407 -1942 566 2015 -3216 -3817 -3156 151\n";
print INPUT_HMMS " - -150 -501 232 43 -382 398 108 -624 212 -467 -721 279 393 46 95 360 116 -368 -295 -250 \n";
print INPUT_HMMS " - -513 -1877 -5220 -27 -5766 -274 -2532 * * \n";
print INPUT_HMMS " 46 -898 -5770 -215 -2255 -6055 -4029 -3320 -5942 -688 -5816 -5099 4098 -4528 -323 -4168 -1085 -4041 -5417 -5997 -5050 153\n";
print INPUT_HMMS " - -149 -500 233 43 -381 398 105 -627 210 -466 -721 279 395 45 96 359 117 -370 -295 -250 \n";
print INPUT_HMMS " - -71 -4965 -5967 -1496 -632 -441 -1925 * * \n";
print INPUT_HMMS " 47 1465 -2262 -4310 -3699 -216 -1932 -2714 522 717 961 703 -3373 -989 1417 -586 -2931 -2306 920 -2712 -2360 157\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9925 -10967 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 48 -264 -3457 -569 -1531 -3710 -248 2089 194 -360 -1367 -2565 -333 -796 78 -1935 1132 -208 1858 -3683 -3045 158\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9925 -10967 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 49 -201 -362 308 65 -3938 -89 1110 -1055 1256 -3633 -2706 949 -3211 1040 857 397 399 -418 -3800 -3118 159\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9925 -10967 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 50 587 412 -2168 519 -3528 27 -1879 -314 409 -452 -2446 1906 -3294 -607 -2003 282 345 681 -3583 -2981 160\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -47 -9925 -5012 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 51 -1168 -3564 -1965 195 -3877 -3087 -1746 61 837 -1026 -2655 1299 1994 1813 1071 -1108 -2049 -846 -3752 -3074 161\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -48 -9880 -4980 -894 -1115 -1586 -585 * * \n";
print INPUT_HMMS " 52 60 -3523 -444 -378 -3834 1183 -1709 -914 -542 -740 -2614 -602 -4 1163 -317 796 -93 842 -3712 -180 162\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9835 -10877 -894 -1115 -1035 -966 * * \n";
print INPUT_HMMS " 53 -922 1618 -2659 -533 -2827 -3394 455 104 -35 859 -1935 -374 128 -484 -871 -864 -329 1621 -3136 1400 163\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9880 -10922 -894 -1115 -1586 -585 * * \n";
print INPUT_HMMS " 54 281 -3568 -506 -413 -3883 -3085 -1744 -822 378 -1689 -2658 934 2283 2094 -1833 -786 -377 -156 -3754 -3075 164\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9880 -10922 -894 -1115 -1586 -585 * * \n";
print INPUT_HMMS " 55 356 1812 -520 14 -752 -230 -1770 -909 200 -893 758 1438 767 643 -364 -1267 48 -652 -3695 -451 165\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -47 -9880 -5011 -894 -1115 -1586 -585 * * \n";
print INPUT_HMMS " 56 1350 -3435 -1989 -1440 -3712 1635 -1749 -69 1825 -3435 -2543 462 -1457 201 -1847 -235 -2029 -455 -3654 -3003 166\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -118 -9836 -3690 -894 -1115 -1959 -429 * * \n";
print INPUT_HMMS " 57 -1988 1088 -1881 956 -3624 -2980 624 -579 2343 -3345 -420 -8 -3072 -1199 673 76 -845 1000 -3559 -2905 167\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -104 -9720 -3869 -894 -1115 -2599 -260 * * \n";
print INPUT_HMMS " 58 -116 -3369 -658 747 -3690 -2872 -1531 -3440 2239 -812 -2459 1920 646 -1071 -31 -332 -1837 -1204 -3553 -2871 168\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -57 -9619 -4750 -894 -1115 -2969 -197 * * \n";
print INPUT_HMMS " 59 607 238 -184 1508 -3645 -2829 -1488 -765 1373 -1981 -2415 55 773 1841 -349 -1736 -684 -1512 -3509 -2827 169\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -97 -9565 -3979 -894 -1115 -3124 -176 * * \n";
print INPUT_HMMS " 60 190 -3255 511 -69 -3576 -45 966 -3327 676 -3271 165 744 700 -229 1184 703 584 -2877 -3439 -2756 170\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -616 -9471 -1530 -894 -1115 -3348 -149 * * \n";
print INPUT_HMMS " 61 -73 -2789 536 -642 -100 -552 -972 477 1452 -2801 995 924 17 677 160 -168 223 -2406 -2977 -2299 171\n";
print INPUT_HMMS " - -154 -492 231 45 -377 395 99 -626 220 -465 -727 281 394 48 93 362 113 -367 -301 -256 \n";
print INPUT_HMMS " - -1010 -1212 -3798 -3050 -186 -233 -2744 * * \n";
print INPUT_HMMS " 62 -653 -3497 310 785 -552 -1256 -1675 -320 1500 -836 -69 -130 801 -178 278 526 -1979 194 -3684 -3005 191\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9795 -10837 -894 -1115 -2222 -348 * * \n";
print INPUT_HMMS " 63 -74 406 767 -1635 393 -1058 821 1002 1145 -203 -2193 310 -1560 -1473 395 -211 -1090 402 -3351 -2791 192\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9795 -10837 -894 -1115 -2222 -348 * * \n";
print INPUT_HMMS " 64 200 240 -360 -1341 574 -430 2490 -3569 615 -3518 -2593 570 -1173 1000 201 777 -1978 746 -3689 -3008 193\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9795 -10837 -894 -1115 -2222 -348 * * \n";
print INPUT_HMMS " 65 -414 -3510 -1887 652 -3830 -1634 -1671 -1689 2011 -577 -2599 -812 425 114 1375 54 1011 -3131 -3693 -3011 194\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9795 -10837 -894 -1115 -1557 -599 * * \n";
print INPUT_HMMS " 66 -2074 -3529 -1921 -307 -3844 -1184 1420 -3589 1136 -3543 -2622 -170 2082 -1252 -1798 781 1588 512 -3718 -3039 195\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9826 -10868 -894 -1115 -2029 -406 * * \n";
print INPUT_HMMS " 67 -265 330 -387 -239 -3828 -3042 -1701 513 1888 -1425 -2608 -150 -1147 295 31 436 526 790 -3705 -3028 196\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9826 -10868 -894 -1115 -1512 -623 * * \n";
print INPUT_HMMS " 68 550 -2518 -423 -707 361 -352 -2270 198 -2309 -975 -1700 -414 -3581 -2175 -2601 760 2098 1036 -2923 -2502 197\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9848 -10890 -894 -1115 -1868 -462 * * \n";
print INPUT_HMMS " 69 -805 -3547 221 -637 -3865 -3058 -1716 -3613 2598 -1415 -2637 1041 -1571 -1258 1767 -1964 -492 -963 1395 -3052 198\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9848 -10890 -894 -1115 -1868 -462 * * \n";
print INPUT_HMMS " 70 2 -3554 -477 -384 -533 -3055 792 -3625 1732 -2150 -2643 644 815 52 368 885 777 -1032 -3737 -3054 199\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -122 -9848 -3643 -894 -1115 -509 -1750 * * \n";
print INPUT_HMMS " 71 -757 -3075 -2197 119 1645 257 1481 547 -548 -443 -2211 -1908 158 -32 -363 564 241 220 -3369 -2808 200\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9812 -10854 -894 -1115 -2121 -377 * * \n";
print INPUT_HMMS " 72 127 -3517 -972 154 -3835 -3028 -1686 1000 1584 -899 -2607 675 -114 80 1818 -675 -1992 -476 -3703 -3022 201\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9812 -10854 -894 -1115 -1426 -672 * * \n";
print INPUT_HMMS " 73 1151 -3551 -658 713 -3872 -1515 -1710 -3622 1222 -757 -2640 -1687 5 1063 1693 552 -1186 -3173 -3734 -3051 202\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9844 -10886 -894 -1115 -1899 -450 * * \n";
print INPUT_HMMS " 74 528 -3551 -326 -380 -3872 351 -1710 -3622 547 -1940 -2640 435 -1456 2314 359 932 -581 -229 -3734 -3051 203\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9844 -10886 -894 -1115 -772 -1271 * * \n";
print INPUT_HMMS " 75 -1744 -2780 -5380 -4764 1230 -4640 -3536 753 -4386 2060 1242 -4289 -4624 -3968 -4183 -3745 1179 1429 -3301 -3001 204\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 76 619 -3599 261 720 -3920 -1045 1509 -3670 1657 -211 -2688 -1735 12 682 -404 252 29 -1510 -3782 -3099 205\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 77 816 -3599 -697 1454 -3920 -1498 -1758 -824 1330 -3615 -2688 522 1038 -16 -22 133 -731 -1102 -3782 -38 206\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 78 467 -3599 643 580 -3920 -3100 1060 -3670 394 -1194 -2688 1485 -3193 774 -370 1127 369 -251 -3782 -3099 207\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 79 879 -3587 1000 -683 -3902 -513 -1762 -286 -116 -1358 -2677 1579 -1505 856 -1851 919 -562 539 -3773 -3094 208\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 80 -280 -2310 -3914 -3321 -2281 -3775 -2599 1645 -337 792 1112 32 -119 1064 -3122 118 -571 1308 -2751 -2385 209\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 81 20 -3578 50 -576 -3889 -3105 204 735 1126 306 -2670 -294 -1261 1229 -181 -169 -731 847 -3767 -126 210\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 82 -892 -3581 -1982 1073 -3894 -3104 -1764 229 1759 -965 -2672 465 1836 -19 69 -968 159 -847 -3769 -3091 211\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 83 -192 -3599 884 1411 -3920 -3100 205 529 1730 -3615 -2688 835 -812 385 583 -780 -2065 -714 -3782 -3099 212\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 84 -121 -3599 -697 1784 -3920 -3100 87 -1384 664 -3615 -2688 1296 1318 714 -370 -803 17 -649 -3782 118 213\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 85 -415 -3599 1417 1609 -3920 -3100 -1758 -3670 532 -1396 -2688 307 776 361 -404 400 301 -149 -3782 -3099 214\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 86 807 -314 -452 441 -3867 -934 -1769 395 191 -807 165 -296 51 -326 759 433 8 527 -3756 -3083 215\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 87 109 -3596 733 -84 -275 -3100 -1759 -3666 1442 -1361 -2686 1721 805 -115 -1847 366 899 -1488 -3780 -3098 216\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 88 71 292 -2967 657 -2642 -1049 -2268 681 1927 276 475 -2521 -701 -2131 -652 -196 -2199 912 -3014 -2582 217\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9903 -10945 -894 -1115 -1348 -720 * * \n";
print INPUT_HMMS " 89 530 -3599 -415 260 -3920 -435 -1758 -3671 1932 -1324 -2688 1169 1022 59 870 -897 -938 -3221 -3782 -236 218\n";
print INPUT_HMMS " - -197 -493 191 93 -373 330 130 -627 301 -436 -643 242 408 56 44 370 156 -366 -348 -310 \n";
print INPUT_HMMS " - -5067 -2315 -378 -6807 -13 -1348 -720 * * \n";
print INPUT_HMMS " 90 -1183 2447 -3481 -2903 -990 -2737 -1788 35 -2541 1696 -132 -2435 985 -2188 -2396 -1864 -1160 1900 -1652 -1320 624\n";
print INPUT_HMMS " - -149 -500 232 42 -378 398 105 -624 213 -467 -721 277 393 45 95 360 117 -368 -295 -250 \n";
print INPUT_HMMS " - -2954 -203 -8832 -35 -5397 -3094 -180 * * \n";
print INPUT_HMMS " 91 -891 -2362 764 730 -2683 -1863 2542 -2433 754 -2378 -1452 1024 465 -63 789 349 -830 -543 -2546 -1863 626\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -8 -8154 -9196 -894 -1115 -3784 -109 * * \n";
print INPUT_HMMS " 92 -1021 -2014 -1053 557 -2180 -2064 -772 323 -461 -235 -1161 1639 1867 -398 -923 1096 -965 -1551 -2315 -1748 627\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -7 -8263 -9305 -894 -1115 -3538 -130 * * \n";
print INPUT_HMMS " 93 114 -2091 321 119 -2258 -2116 -799 211 -472 -341 1463 866 -2204 -415 -939 1712 375 -1628 -2374 -1800 628\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -6 -8401 -9443 -894 -1115 -2357 -313 * * \n";
print INPUT_HMMS " 94 -53 -2745 316 1417 -3057 -503 -928 -2802 579 -2757 -1836 -905 403 -471 -1018 1385 -1231 619 -2933 606 629\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -5 -8790 -9832 -894 -1115 -2830 -219 * * \n";
print INPUT_HMMS " 95 -1503 -2425 -1585 1441 -2596 473 -1293 -153 -1009 -2394 -1600 170 791 -937 -1464 453 -1454 1987 -2763 -2211 630\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -8949 -9991 -894 -1115 -2993 -194 * * \n";
print INPUT_HMMS " 96 -225 -3138 848 1211 -3454 -2568 -1297 -3204 -922 -3160 -2251 371 1161 -850 -1442 1855 -1624 853 -3340 -2648 631\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9051 -10093 -894 -1115 -3975 -95 * * \n";
print INPUT_HMMS " 97 -136 -2917 -1328 552 -247 -2449 -1108 -2970 930 -176 -2009 363 1290 680 -1199 1511 28 -2533 -3107 -2431 632\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9051 -10093 -894 -1115 -2984 -195 * * \n";
print INPUT_HMMS " 98 -1536 -2924 -1418 594 -170 -750 -1186 -646 1248 553 -2022 -1173 1449 631 183 427 13 -2527 -3128 -2467 633\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9135 -10177 -894 -1115 -3882 -101 * * \n";
print INPUT_HMMS " 99 -461 1243 1155 1373 -3321 -403 -1163 -3072 1272 -993 -2091 -1140 -311 650 -1251 842 114 -2623 -3185 -2503 634\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9135 -10177 -894 -1115 -3882 -101 * * \n";
print INPUT_HMMS " 100 -1530 862 119 1554 -3316 -2505 1903 -439 254 -3012 -2087 790 1470 1283 -1252 -1412 -135 -2618 1656 -2501 635\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9135 -10177 -894 -1115 -2755 -231 * * \n";
print INPUT_HMMS " 101 265 -2898 -213 1904 -3145 -2620 -1288 325 1539 -120 -2007 -1288 -249 -856 -1395 -295 -1554 218 -3127 -2492 636\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9230 -10273 -894 -1115 -3761 -111 * * \n";
print INPUT_HMMS " 102 689 -2324 713 -1477 655 -2832 -1546 75 -132 -141 -1484 -1683 -226 404 -1761 939 1387 -289 -2676 -2186 637\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9230 -10273 -894 -1115 -3761 -111 * * \n";
print INPUT_HMMS " 103 -457 -2676 -1691 546 -2846 85 995 888 -1041 -1112 -1807 -1411 -2776 1992 21 -258 -26 1334 -2957 -2380 638\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9230 -10273 -894 -1115 -3761 -111 * * \n";
print INPUT_HMMS " 104 -304 -3067 1378 72 950 -425 -1234 -744 1505 -3082 727 998 -112 -775 711 -277 -1539 -2688 -3252 -2571 639\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9230 -10273 -894 -1115 -3244 -161 * * \n";
print INPUT_HMMS " 105 -391 782 798 1350 893 -2632 -1297 -720 49 252 -2069 -1292 -274 464 -138 -246 698 -1216 -3183 -2537 640\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9264 -10306 -894 -1115 -2411 -301 * * \n";
print INPUT_HMMS " 106 193 -3111 1094 1377 -3402 -2696 934 583 1521 -312 -2208 -1343 -2789 55 -1453 -334 -1650 -659 1384 -2648 641\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9371 -10413 -894 -1115 -3073 -183 * * \n";
print INPUT_HMMS " 107 543 -3161 21 502 -502 -2709 -1369 687 1467 -1122 -2255 73 -414 1504 -1461 230 -1668 13 -3355 -2682 642\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9398 -10440 -894 -1115 -3495 -134 * * \n";
print INPUT_HMMS " 108 -1751 832 -354 463 -3124 -2779 -1452 211 603 -204 -2037 -1471 -2868 1229 20 -378 407 1903 -3172 -2565 643\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9398 -10440 -894 -1115 -2830 -219 * * \n";
print INPUT_HMMS " 109 -801 -3230 1599 1274 -3551 -2730 -1389 -3302 1184 -181 -2319 186 -1137 622 -80 724 322 -2852 -3413 -2730 644\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9438 -10480 -894 -1115 -3418 -142 * * \n";
print INPUT_HMMS " 110 -523 -3180 372 1163 -3479 -2743 -1403 152 -391 -892 -334 1249 545 -951 -41 976 -185 391 -3377 -2708 645\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9438 -10480 -894 -1115 -2892 -209 * * \n";
print INPUT_HMMS " 111 -376 -3226 406 2305 -3535 -2759 862 -682 -80 -1179 551 -1399 -2852 383 1155 -1667 212 -427 -3416 -2741 646\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9467 -10509 -894 -1115 -3357 -148 * * \n";
print INPUT_HMMS " 112 -130 -3249 93 2539 -3569 78 -1412 -731 -130 -1187 -2339 -1389 375 749 -103 -1660 -145 -2871 -3433 -2751 647\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9467 -10509 -894 -1115 -2037 -403 * * \n";
print INPUT_HMMS " 113 246 956 -434 1348 -907 512 -1853 -13 -1704 544 -1680 -2017 -865 189 -2088 87 190 412 -2880 -2406 648\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9563 -10605 -894 -1115 -2659 -249 * * \n";
print INPUT_HMMS " 114 56 -3345 1339 1316 -3666 -1078 -1505 -3417 1060 -1999 -2435 -1481 1103 -1045 935 632 103 -2967 -3529 -2846 649\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9586 -10629 -894 -1115 -3064 -184 * * \n";
print INPUT_HMMS " 115 751 -3049 -237 -337 567 -1093 -1606 -2923 -1249 -705 -2171 -1627 1943 -1198 508 1013 315 -65 -3308 -2705 650\n";
print INPUT_HMMS " - -151 -503 229 41 -384 397 110 -622 217 -461 -717 272 393 47 94 359 122 -373 -298 -253 \n";
print INPUT_HMMS " - -50 -4914 -10629 -4891 -49 -1932 -439 * * \n";
print INPUT_HMMS " 116 -935 1136 2187 213 -326 -2921 650 -3344 1353 -1488 -2425 -278 -3013 -1134 -1678 709 -1873 871 -3531 -2868 687\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 117 -89 700 106 942 -407 -962 714 -3468 389 -1611 -2487 -47 -576 1234 -1649 1517 23 -565 -3582 -2900 688\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 118 279 -3399 898 245 -3720 -926 703 -493 1227 -1228 -2489 -267 2187 -1101 -845 57 -696 -3021 -3583 -2901 689\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 119 -619 -3400 898 1297 -3721 -2901 -1560 -3471 900 -1508 1567 -419 -357 225 460 1383 -364 -1234 -3584 -2901 690\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 120 -1949 -3418 -1789 -237 -696 11 -1576 -3489 388 -3433 -2508 844 2967 920 276 121 -1887 -3041 -3599 -2919 691\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 121 -308 -1983 -4458 -874 -169 -3694 -2563 1949 -3428 801 2279 -3328 -1001 -3058 -601 -862 294 1298 -2440 -2097 692\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9657 -10699 -894 -1115 -2842 -217 * * \n";
print INPUT_HMMS " 122 -935 -3326 -1814 437 -682 -929 650 -3341 418 -1194 -2424 -1570 1709 1673 -1679 1177 -356 62 -3529 51 693\n";
print INPUT_HMMS " - -159 -561 197 165 -320 305 84 -634 248 -383 -651 256 366 55 23 379 152 -400 -388 -332 \n";
print INPUT_HMMS " - -2327 -3271 -520 -8400 -4 -2842 -217 * * \n";
print INPUT_HMMS " 123 -1048 -905 -3157 -2548 -832 -2584 -1475 -173 -2201 543 2885 -2159 807 -1868 -2092 -1668 1538 1558 -1399 -1060 1241\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -9 -7937 -8979 -894 -1115 -4617 -60 * * \n";
print INPUT_HMMS " 124 -877 -841 -2522 456 -802 -2334 -1163 991 -1656 447 1844 -1728 -2391 1834 -1698 -1384 -817 919 -1284 1501 1242\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -845 -7937 -1188 -894 -1115 -4617 -60 * * \n";
print INPUT_HMMS " 125 1077 -1188 1409 -328 -1554 -1494 -502 -961 -296 -1342 -599 -510 -1714 -197 -717 -549 1229 1139 -1804 -1294 1243\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -338 -7108 -2310 -894 -1115 -3990 -94 * * \n";
print INPUT_HMMS " 126 2211 -799 -2287 -1977 -1912 -1332 -1619 -1186 -1798 -1749 -1055 -1460 -1917 -1581 -1920 1354 -609 1596 -2309 -1967 1244\n";
print INPUT_HMMS " - -149 -500 233 44 -381 398 105 -623 210 -466 -721 275 393 45 98 359 117 -368 -295 -250 \n";
print INPUT_HMMS " - -2241 -350 -8119 -61 -4590 -491 -1794 * * \n";
print INPUT_HMMS " 127 -1853 -3150 -1774 1648 -3402 -959 1293 1123 -25 -707 -2257 57 -2947 -9 -1628 -287 1367 624 -3376 -2737 1246\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9544 -10586 -894 -1115 -922 -1082 * * \n";
print INPUT_HMMS " 128 -1019 -3468 -1870 1789 -3781 -2992 717 -812 1057 -3480 1568 -1630 891 -1194 -313 1547 -26 -3085 -3656 -2978 1247\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9763 -10805 -894 -1115 -2400 -303 * * \n";
print INPUT_HMMS " 129 -2012 -3485 1726 471 -3806 596 498 -3557 1573 -1403 -2574 91 126 97 -175 424 -515 -1396 -3668 -2986 1248\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9763 -10805 -894 -1115 -1439 -664 * * \n";
print INPUT_HMMS " 130 143 2787 226 -407 -3381 -3126 -1803 -187 817 -720 -2318 -1832 552 -1404 843 -122 -534 1208 -3460 -2868 1249\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9813 -10855 -894 -1115 -2115 -379 * * \n";
print INPUT_HMMS " 131 -800 -3479 825 -329 -455 -3038 1484 -3516 806 -380 915 -163 -835 -1246 -303 1112 579 124 -3675 460 1250\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -51 -9813 -4891 -894 -1115 -2115 -379 * * \n";
print INPUT_HMMS " 132 -2039 -3225 -2014 251 -3439 -3062 -1735 701 358 -844 1993 -218 497 803 -225 466 589 1131 -3476 -2864 1251\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9764 -10806 -894 -1115 -2393 -305 * * \n";
print INPUT_HMMS " 133 -169 -3486 630 1516 463 -2987 -1646 -3557 1902 -1302 -2575 -280 219 1508 -1734 -448 -614 -3108 -3669 -2987 1252\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9764 -10806 -894 -1115 -2393 -305 * * \n";
print INPUT_HMMS " 134 315 -3479 -335 2507 -796 -2989 -1648 -861 1367 -3494 -2569 -15 -474 138 -1736 -1896 270 -987 -3665 -2984 1253\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9764 -10806 -894 -1115 -2393 -305 * * \n";
print INPUT_HMMS " 135 345 -2865 -15 2040 676 -1168 -1902 720 -314 255 -2017 -2000 -3284 -1583 -2086 -103 324 -683 -3195 -2677 1254\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -26 -9764 -5883 -894 -1115 -2393 -305 * * \n";
print INPUT_HMMS " 136 -1997 -3442 1006 398 -1335 46 -1635 19 818 -3453 -2534 -773 2503 -1179 -1726 -411 721 -1274 -3633 -2957 1255\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -48 -9740 -4991 -894 -1115 -2509 -279 * * \n";
print INPUT_HMMS " 137 -119 -2198 -3463 -714 -2179 -3537 -2341 -91 7 1738 1191 -2803 1193 -424 -2802 -972 300 782 -2631 -2252 1256\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -278 -9695 -2521 -894 -1115 -2702 -241 * * \n";
print INPUT_HMMS " 138 149 1871 -1627 1486 -3431 -664 804 -3159 617 -1128 1228 -1383 -2827 509 687 296 -1688 -818 -3345 1105 1257\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9420 -10462 -894 -1115 -3453 -138 * * \n";
print INPUT_HMMS " 139 222 -1981 -3201 2257 1283 -3301 -2103 132 -258 -639 -1174 -2554 -347 -2204 -2556 -547 -252 554 1682 -2031 1258\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9420 -10462 -894 -1115 -3453 -138 * * \n";
print INPUT_HMMS " 140 -1746 -3176 -1610 596 -3480 -127 -1387 1275 1593 -1193 -2270 87 -2819 404 -46 939 -98 47 -3370 -2698 1259\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9420 -10462 -894 -1115 -3453 -138 * * \n";
print INPUT_HMMS " 141 445 -3203 -1631 1780 -3508 -2748 839 -899 679 -1858 -2296 849 -2841 -954 68 567 -269 957 -3396 -2723 1260\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -66 -9450 -4528 -894 -1115 -896 -1112 * * \n";
print INPUT_HMMS " 142 -195 3704 -2100 610 -3128 -3071 650 -919 821 -1258 202 -144 -3158 -1387 -1906 486 804 -2505 -3272 1017 1261\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9693 -10735 -894 -1115 -2805 -223 * * \n";
print INPUT_HMMS " 143 372 -3424 1770 973 -3742 -2932 -1590 -3491 894 -1816 1000 146 -3025 2198 -1679 -1838 -460 -56 -3608 -2927 1262\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9693 -10735 -894 -1115 -2805 -223 * * \n";
print INPUT_HMMS " 144 1825 -3363 951 1016 -3654 -2947 -1609 489 -263 -557 138 -1594 -509 -20 -183 -20 -1901 -911 -3564 -2900 1263\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -35 -9693 -5440 -894 -1115 -2805 -223 * * \n";
print INPUT_HMMS " 145 -561 -3335 718 650 2084 -2922 -1583 -759 -1176 -3338 -2432 1251 1227 -1134 -263 551 314 -1107 -3537 -2873 1264\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9660 -10702 -894 -1115 -2919 -205 * * \n";
print INPUT_HMMS " 146 492 2077 -680 -1771 -2784 -111 -1863 -502 -288 -2610 288 -1987 261 75 -2067 1733 200 559 -3046 41 1265\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -108 -9660 -3818 -894 -1115 -2919 -205 * * \n";
print INPUT_HMMS " 147 117 -3319 1171 383 -3640 -2821 -1479 -3391 842 784 -2409 1066 -628 1032 -111 68 -304 -1049 -3503 -2820 1266\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9555 -10597 -894 -1115 -3220 -164 * * \n";
print INPUT_HMMS " 148 622 -3319 819 1654 -3640 -967 794 -3390 -42 -3335 -2408 -1457 -622 -1020 -111 915 109 823 -3503 -2820 1267\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9555 -10597 -894 -1115 -3220 -164 * * \n";
print INPUT_HMMS " 149 -614 -3215 514 1675 -3489 -2849 -1512 36 480 1247 -2316 -1503 -630 153 601 -1760 -1796 -788 1315 -2772 1268\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -165 -9555 -3230 -894 -1115 -3220 -164 * * \n";
print INPUT_HMMS " 150 1045 988 1496 440 -546 -2794 -1471 -1190 -1126 757 -1981 -203 -2883 965 -199 -1716 -540 -2404 -3124 1092 1269\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -534 -9393 -1699 -894 -1115 -2627 -255 * * \n";
print INPUT_HMMS " 151 984 1761 204 1114 -3185 -397 1778 -2934 961 -788 -1956 -1010 -2467 -574 322 652 -1338 -2487 -3051 -2370 1270\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -388 -8952 -2098 -894 -1115 -4109 -86 * * \n";
print INPUT_HMMS " 152 1636 1724 505 1318 -2575 -258 -857 -2257 -491 -510 -1476 -869 -2274 831 -976 -1102 -1102 64 -2608 -1994 1271\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -321 -8570 -2346 -894 -1115 -4379 -71 * * \n";
print INPUT_HMMS " 153 -1008 -1562 -1465 1256 1690 189 -919 -1208 -774 -1460 1449 -1089 -2274 -683 1493 -1157 481 256 -1930 -1459 1272\n";
print INPUT_HMMS " - -150 -501 232 45 -382 398 105 -624 211 -465 -721 274 396 44 97 361 116 -370 -295 -250 \n";
print INPUT_HMMS " - -3420 -144 -9298 -24 -5901 -4530 -64 * * \n";
print INPUT_HMMS " 154 -968 -2407 -818 606 -2718 -62 -601 -2458 2151 -2418 -1501 -582 509 1300 -679 -853 598 222 -2597 -1924 1274\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -117 -8256 -3739 -894 -1115 -4530 -64 * * \n";
print INPUT_HMMS " 155 1110 -2209 -809 1379 -2458 -1910 -584 -2162 -198 -2195 1348 1053 -2006 1207 -690 -833 -857 -1793 -2435 2005 1275\n";
print INPUT_HMMS " - -148 -501 232 44 -378 398 105 -625 213 -467 -721 277 393 44 95 360 116 -370 -295 -246 \n";
print INPUT_HMMS " - -3311 -156 -9189 -26 -5783 -4573 -62 * * \n";
print INPUT_HMMS " 156 -935 -2387 -763 1353 -2710 -1897 -556 -2453 -121 -2401 -1483 -536 1534 1302 1571 438 519 -2012 -2570 -1897 1277\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -8 -8146 -9189 -894 -1115 -166 -3204 * * \n";
print INPUT_HMMS " 157 -185 -3576 704 705 -3897 -307 -1735 -3648 1153 -3592 -2665 885 1787 921 -352 587 -559 -3198 -3759 -3076 1278\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9873 -10915 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 158 -2328 -2192 -4440 -30 -180 -3849 571 1642 -3464 -2042 143 -3416 -592 -3131 -49 -985 142 2091 3422 -2298 1279\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9873 -10915 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 159 -2126 -3329 883 1900 -614 -3146 -1818 323 772 485 25 -859 -696 41 -1935 -739 -547 -323 -3576 28 1280\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9873 -10915 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 160 -4438 -6572 3803 345 -6653 -4125 -3597 -6671 -1053 -6485 -5959 1438 -4729 -3295 -5119 -4022 -4547 -6096 -6692 -5514 1281\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9873 -10915 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 161 -2742 -2531 -5106 -4489 -2456 -4354 -3252 2960 -4096 1204 -1630 -3999 205 -3722 -723 -3453 -2685 1196 -3079 153 1282\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9873 -10915 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 162 -4026 -5883 3782 168 -6049 -4008 -3318 -896 -3509 -1599 -5138 -304 -4520 725 -4324 -3701 -4080 -5440 -6035 -5053 1283\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -92 -9873 -4044 -894 -1115 -1841 -472 * * \n";
print INPUT_HMMS " 163 1812 -3442 1134 1394 -3736 -3020 -1681 -3468 -1273 -1282 660 142 -3112 -26 -1776 686 -1975 -1209 -3642 821 1284\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9784 -10826 -894 -1115 -1387 -695 * * \n";
print INPUT_HMMS " 164 -243 1288 1754 2035 -3768 -1395 566 -857 -250 -1732 -2575 -1712 -3157 -1277 -141 65 -2018 358 -3679 -433 1285\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9838 -10880 -894 -1115 -2100 -383 * * \n";
print INPUT_HMMS " 165 -2312 -3800 3256 1307 -4110 -3198 432 -3867 -1539 -1460 -2897 -193 -3335 -1470 -255 -245 -2257 -3417 -3982 193 1286\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -42 -9838 -5194 -894 -1115 -2100 -383 * * \n";
print INPUT_HMMS " 166 590 776 -1460 -2619 -2397 -78 -2304 -1957 1199 -471 613 -2654 -3596 -2283 -1378 1325 411 934 2131 -715 1287\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9799 -10841 -894 -1115 -350 -2216 * * \n";
print INPUT_HMMS " 167 335 -3634 1731 1084 -1052 180 444 -3705 56 -3650 -2723 1629 -3228 10 -1882 233 -517 -904 -3817 -210 1288\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -38 -9946 -5316 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 168 -4464 -6569 3166 -1202 -6672 -4160 -3635 -6694 -4101 -6513 -202 3298 -4765 -3334 -5155 -4054 -4576 -6118 -6716 -5544 1289\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9910 -10952 -894 -1115 -1503 -628 * * \n";
print INPUT_HMMS " 169 -2306 -3533 184 502 -3763 -3267 539 -893 -1638 -3515 -2661 -1948 3382 -1569 -2134 100 -588 -171 -3785 -3158 1290\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9910 -10952 -894 -1115 -1503 -628 * * \n";
print INPUT_HMMS " 170 -861 -2297 -713 -3420 1217 -3801 -2632 -1810 -3146 1577 1098 307 -3859 1791 -3177 690 -2281 596 -2741 -194 1291\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9910 -10952 -894 -1115 -1503 -628 * * \n";
print INPUT_HMMS " 171 1393 -2237 -4383 -1035 -400 -3867 274 -1737 -3430 1235 3544 -1359 -3920 -3112 -3342 159 -2297 -295 -2689 -2339 1292\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9910 -10952 -894 -1115 -360 -2181 * * \n";
print INPUT_HMMS " 172 1619 3932 -5208 -4723 -3037 -3978 -3564 -493 -4351 -2906 -2264 1104 -4322 -3979 -4175 -807 -816 1933 -3540 -3208 1293\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -24 -9968 -6024 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 173 285 -2439 -3753 -18 -2438 -3775 -2618 -1044 -2950 -1222 -1650 -3078 673 -2743 -3084 2087 412 1952 -2894 -2517 1294\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9946 -10988 -894 -1115 -1060 -942 * * \n";
print INPUT_HMMS " 174 -777 -4857 1774 3309 -5129 -3825 -2900 -953 -2803 -4880 52 -2465 -524 -2520 -3427 -3193 -3384 -4452 -5128 -4355 1295\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9946 -10988 -894 -1115 -514 -1738 * * \n";
print INPUT_HMMS " 175 -6101 -5259 -6918 -1220 159 -6744 -3166 -4618 -6669 -1448 1202 -5492 -6575 -5524 -6113 -5977 -5971 -4955 -2417 4682 1296\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 176 2241 -2979 -765 -4802 -3404 -4193 -3904 -81 -4538 -3138 -2568 -4203 -4561 -4207 -4450 -238 -724 2680 -3967 -3624 1297\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 177 -871 -3652 646 871 -610 -3153 152 -3723 2242 -3668 738 1150 187 978 -1900 -71 -2118 -1063 -3835 -3153 1298\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 178 -4452 -6450 3591 1898 -6691 -963 -3685 -6695 -4137 -6526 -5973 -2802 -4802 -3382 -5175 -1126 -909 -6101 -6732 -5582 1299\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 179 -4724 -4173 -7340 -6943 -3602 -7202 -6682 3791 -6869 -576 1777 -6880 -6633 -6232 -6741 -6599 -4674 -435 -5449 -5412 1300\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 180 -6579 969 -6989 -7335 3575 -6863 138 -5469 -6890 -1267 -4877 -5482 -6719 -5619 -6257 -6109 -6431 -5623 -2308 3659 1301\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 181 388 1786 951 1168 -1089 -3155 550 -1088 533 -1467 -2734 1110 -3248 250 -1903 954 -243 -3264 -3830 -227 1302\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 182 -6639 -5546 -6999 -7361 2566 -6884 1588 -5523 -6916 -4824 -4923 -5490 -6738 -5632 -6276 -6134 -6487 -5680 -2307 4398 1303\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 183 -5623 -4979 -7950 -7365 -2904 -7769 -6139 -569 -7160 2471 3067 -7449 -6705 -5821 -6580 -7159 -5443 -3356 -4678 2820 1304\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 184 -3032 -4322 -3006 -495 -4776 -3913 1249 -4417 1928 -4274 -3422 -405 -3964 -29 3095 -2911 -2916 -318 -4331 796 1305\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 185 -889 -3654 -678 1770 -3975 -3154 2165 -3726 1408 -3670 -2743 602 -3247 1922 93 112 -70 -3276 -3837 -3154 1306\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 186 -2417 -2302 -4417 -3798 -544 -3921 1164 -1803 -920 2019 1627 -940 -3974 -3142 1929 -2991 -915 857 -2752 -2402 1307\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 187 -4558 -6474 -366 3828 -6739 -4283 -3789 -6718 -4254 -6604 -6085 -2902 -4890 -3494 -5281 -4177 -4686 -1251 -6760 -5666 1308\n";
print INPUT_HMMS " - -149 -500 233 43 -381 400 105 -626 212 -466 -721 275 394 45 96 359 117 -369 -295 -250 \n";
print INPUT_HMMS " - -72 -4858 -6116 -985 -1015 -701 -1378 * * \n";
print INPUT_HMMS " 188 -1222 245 -516 1490 -3931 -1482 -1800 904 919 -375 -2709 82 -567 1 592 -842 -1185 1045 -3806 -3128 1311\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -1040 -961 * * \n";
print INPUT_HMMS " 189 -622 -3606 -2031 1680 -35 -3148 584 -189 2006 -390 -2699 -1790 -3241 1643 604 -790 -2108 -3221 -3798 -3125 1312\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -523 -1717 * * \n";
print INPUT_HMMS " 190 -2411 784 -4508 -3888 2334 -1245 529 -1775 -3536 -927 543 -3492 -3982 2080 -3424 921 941 -1693 1032 1686 1313\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 191 718 2235 -3703 -3120 -2408 -1229 -2576 -677 -79 1170 1448 -3040 9 -303 1111 360 -2309 25 -2861 -2482 1314\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 192 -4347 -3844 -7001 -6645 -4030 -6779 -6530 1051 -6569 541 -2764 -6459 3000 -6301 -6611 -6135 -4328 2344 -5740 -5426 1315\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 193 -2180 448 348 -565 -3974 -3153 -1812 -3724 658 -1415 -2742 1855 1348 637 1872 261 16 -3275 -3836 -3153 1316\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -466 -9968 -1862 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 194 -1834 -3268 -126 446 -3572 -2807 1687 -3311 555 -1481 -2362 -1448 3160 -193 -1560 -1721 -1773 -998 -3460 442 1317\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9504 -10547 -894 -1115 -92 -4016 * * \n";
print INPUT_HMMS " 195 -945 1126 1639 -482 -3971 -3154 1611 -3721 1164 -3666 1125 2156 -3247 -1353 579 -784 -607 -3272 -3834 674 1318\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 196 -6554 -5547 -954 -6861 1358 -6776 766 -5523 -6783 -4833 -4924 -701 -6678 -5553 -6237 -6064 -6417 -5671 -2325 4619 1319\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 197 -5675 -5010 -8052 -7453 -2964 -7928 -6483 774 -7275 1853 4529 -7676 -6765 -5886 -6677 -7367 -5492 -3290 -4844 -5125 1320\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 198 -124 -3619 1845 1158 -3925 -1149 2292 -3666 -1408 -1095 982 353 -3255 -32 -1913 26 -2122 374 -3812 609 1321\n";
print INPUT_HMMS " - -149 -500 236 44 -381 398 105 -627 212 -466 -721 275 393 45 95 359 117 -370 -295 -250 \n";
print INPUT_HMMS " - -191 -3021 -11010 -53 -4793 -701 -1378 * * \n";
print INPUT_HMMS " 199 -699 -3642 -609 -1483 -3958 1776 -1815 -947 1470 -3656 7 705 -3249 1305 182 -195 72 -99 -3828 -3148 1323\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -408 -9968 -2028 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 200 -3813 -4613 -3103 -3203 -5058 -4193 2339 -5658 -2493 -5421 -4801 -3302 -4662 4280 -2576 -3782 -461 -5107 -4957 -4344 1324\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9563 -10605 -894 -1115 -102 -3878 * * \n";
print INPUT_HMMS " 201 -2208 -3370 -2196 -1646 -899 -391 1002 -354 1300 -3336 951 -1931 1842 1290 392 375 531 -1008 -3626 -635 1325\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 202 -3493 -5171 2254 2893 -5424 -3861 -2935 -5249 -722 -5159 -4333 1297 -698 -2543 -3492 -3259 -1412 -4766 -5337 131 1326\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3505 -9968 -135 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 203 -2421 -2043 -2715 -2793 870 -2772 -422 -1944 -2485 -1614 -1542 -2131 -2981 -2125 -2335 -2438 -2456 -1991 187 4469 1327\n";
print INPUT_HMMS " - -149 -500 233 43 -381 398 110 -626 210 -466 -721 275 394 45 96 359 117 -369 -295 -245 \n";
print INPUT_HMMS " - -1651 -565 -7529 -102 -3870 -31 -5573 * * \n";
print INPUT_HMMS " 204 -3729 -3419 -6139 -5555 -2943 -5497 -4431 2861 -5221 1738 559 -5151 -1056 -4726 -5008 -4639 -3664 1167 -4013 164 1329\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 205 -877 -3653 745 -1484 -3973 -3158 369 -3722 232 -3669 -2743 2714 -3252 -1358 269 71 1966 -833 -3838 -3156 1330\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 206 -2182 -3652 -760 1692 -3972 958 -1815 -3722 81 -3668 -2742 -1791 1045 598 -1904 621 175 -957 3306 -3154 1331\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 207 -2189 -3662 884 278 -3983 -3160 605 -3734 2203 -3678 -2752 1649 -768 -1360 1508 322 -2128 -977 -3845 -3162 1332\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -83 -9968 -4182 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 208 -4387 -4433 -6814 -6777 670 -5452 -4571 -3161 -6400 -2377 5116 -5664 -5788 -5583 -5996 -4828 252 -3693 -3838 -2970 1333\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9887 -10929 -894 -1115 -302 -2405 * * \n";
print INPUT_HMMS " 209 -7897 -6692 -7584 -7675 -8060 -6650 -6834 -8988 -6576 -8353 -8200 -7615 -7061 -7168 4270 -8272 -7969 -8674 -6813 -7875 1334\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 210 2584 -3651 134 -428 -3966 17 549 -3712 -1414 -1640 -2744 -1805 -3262 -1372 672 619 -605 -3270 -3840 -3161 1335\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 211 -3580 -3246 -6067 -5544 -3414 -5478 -4600 3666 -5255 -621 529 -5136 -5418 -4936 -5139 -993 14 -173 -4338 -3983 1336\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 212 -5670 -5010 -8048 -7452 -2965 -7903 -6478 -2438 -7271 3268 553 -7664 -6761 -5886 -6674 -7342 -5489 -664 -4843 -5120 1337\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 213 -4048 -3595 -6677 -6297 -813 -6342 -5968 2683 -6168 -3154 -2997 -671 -6185 -6033 -6227 -5633 -878 2999 -5570 -5090 1338\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 214 -4543 -6673 3932 86 -6761 -4225 -3704 -6787 -4186 -6601 -6081 1057 -4832 -3404 -5262 -4127 -4656 -6209 -6803 -5623 1339\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 215 -8386 -6773 -7706 -8090 -7143 -6750 -7075 -9165 -8250 -8440 -8473 -8060 -7177 -8109 -7669 -8910 -8511 -8949 6314 -6875 1340\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 216 -5685 -5017 -8060 -7459 -2963 -7941 -6490 1363 -7283 2946 2257 -7689 -6769 -5887 -6681 -7382 -5499 -3302 -4845 -5130 1341\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 217 -4299 -3775 -7002 -6698 -4437 -6886 -7019 2076 -6691 -1247 -3111 -6541 -6589 -6655 -6884 -6287 -4291 3424 -6287 -5769 1342\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 218 -4302 -6276 327 2935 -6426 -829 -3528 -6369 -66 -6213 -5576 -2740 -4708 3016 -4656 -3938 -4375 -5831 -6403 -5352 1343\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 219 -4302 -3778 -7004 -6699 -4428 -6886 -7014 1516 -6691 -1312 -3103 -6542 -6589 -6650 -6882 -6287 -4293 3579 -6276 -5763 1344\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 220 -1014 -4800 -3568 -3779 -5496 -4496 4849 -6277 -3459 -6099 -5463 -3839 -5073 2779 -3567 -4083 -4305 -5541 -5549 -4886 1345\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 221 -2291 -2798 -231 1879 -442 -1145 -2224 403 -2108 -362 825 275 -3571 -2009 -641 999 -423 632 -3173 422 1346\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 222 -2382 905 -2249 -394 -4165 -3337 553 -3894 2712 -3821 -2910 1463 -3422 -1492 1928 -252 -556 -3456 -3965 -3322 1347\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 223 -6586 -5524 -7007 -7354 4459 -6882 -3087 -636 -6914 -4630 -4746 -5507 -6731 -5636 -6277 -6136 -6440 -5537 -2332 566 1348\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 224 -2199 609 24 2162 -3994 -372 1758 -3744 142 -3688 -2762 -78 -3263 2129 1478 -2078 -2138 -3295 -3854 -3172 1349\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 225 -5616 1762 -8007 -7431 -2972 -7697 -6416 -2482 -7229 3192 1314 -7556 -6712 -5872 -6638 -7135 -5461 -3364 -4831 -5083 1350\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 226 -2428 -2257 -4748 -4114 -2208 -3973 -2843 -654 -3714 2086 2313 1746 -4023 -3342 -3523 -1155 1135 -392 -2715 5 1351\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 227 -2834 -4330 -2078 -143 -4635 -3566 2956 -4394 -2037 -1551 -3453 123 2989 2163 -2549 -814 -2791 -3950 -4507 -3797 1352\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 228 -5053 -6674 -333 3883 -7083 -4676 -4221 -7238 -4712 -7008 -6533 -3362 -5269 -3952 -5692 -4667 -5170 -6674 -6830 -6075 1353\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 229 -3135 -3736 -6088 -6439 -6371 -4002 -5669 -6260 -6282 -6505 -5558 -4786 -4810 -5765 -5948 -759 4017 -4953 -6535 -6518 1354\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 230 -5731 -5055 -8078 -7479 -374 -7958 -6395 -2483 -7297 3211 1743 -7687 -6774 -5879 -6679 -7401 -5539 -3399 -4786 -4981 1355\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 231 -6624 -5542 -6990 -7350 2467 -6876 1313 -5520 -6908 -4823 -4920 -5486 -6733 -5629 -6271 -1908 -6476 -5675 -2307 4418 1356\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 232 -5475 -4954 -7541 -7134 -2983 -7303 -6128 -2536 -6840 3001 3008 -526 -6595 -5765 -6381 -6739 -5364 -3403 -4765 -4864 1357\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 233 2373 2334 -6388 -6726 -6301 -1550 -5653 -6121 -6335 -6385 -5411 -4718 -4688 -5759 -5928 10 2847 -4798 -6526 -6515 1358\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 234 -4281 -3762 -6984 -6690 -4516 -6843 -7039 2907 -6686 -3248 -3186 -6520 -6581 -6688 -6894 -6251 -4277 3054 -6360 -5797 1359\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 235 991 -3754 -3034 -2690 334 -1181 -3000 -4586 1645 -4627 -3745 3376 -4021 -2608 -3085 -409 -2899 -3999 -4826 -4251 1360\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 236 -4663 -4171 -7174 -6678 -3279 -6839 -5917 3049 -6485 1711 282 -6513 -6344 -5754 -6239 -6123 -4589 1117 -4922 36 1361\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 237 -4301 -3826 -6893 -6470 -3735 -6584 -5992 2510 -6322 1037 3095 -6244 -6277 -5929 -6260 -5875 23 1658 -5282 -5044 1362\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 238 -7685 -6760 4229 -6476 -8101 -6473 -6787 -9101 -7552 -8490 -8398 -6748 -6945 -7125 -7457 -7808 -7836 -8719 -6906 -7923 1363\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 239 -4288 745 -5918 -4378 -4222 -5135 1718 -4006 -1954 -688 488 -4094 -5102 -2914 3868 -4470 -4081 -4044 -4331 -3983 1364\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 240 -6480 -5466 -7023 -7326 4093 -6891 -3133 -886 -6894 -4220 79 -5538 -6712 -5626 -6267 -6141 -6331 -5294 -2375 2838 1365\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 241 -5709 -5038 -8078 -7473 -2956 -7960 -6491 -558 -7294 3280 524 -7711 -6774 -5885 -6683 -7404 -5520 -3349 -4838 -5125 1366\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 242 710 2242 -3877 -3303 -2516 -1250 -2713 -2067 -3069 -2399 1677 -3169 -3905 2444 -3186 2113 -2386 -608 -2971 2 1367\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 243 -241 -3634 -144 -64 -3946 -3158 242 -892 2184 204 -2725 -882 -3251 679 -431 -1179 -2120 1582 -3822 -3145 1368\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -721 275 394 45 96 359 119 -369 -294 -249 \n";
print INPUT_HMMS " - -32 -5550 -11010 -978 -1023 -701 -1378 * * \n";
print INPUT_HMMS " 244 -304 -3653 -2028 534 -3975 -3154 1864 -3725 1685 -3669 -2743 1469 -3248 2022 284 -885 538 -356 -3836 -3154 1371\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 245 -329 -190 -3332 -2760 -2542 -3671 -2447 -560 572 -856 403 318 1906 1219 -2823 -570 58 1794 -2959 -2555 1372\n";
print INPUT_HMMS " - -149 -500 233 43 -381 398 109 -626 210 -466 -721 278 394 45 96 359 117 -369 -295 -249 \n";
print INPUT_HMMS " - -84 -4162 -11010 -110 -3770 -701 -1378 * * \n";
print INPUT_HMMS " 246 -3889 2944 -6607 -6267 -4251 -5841 -5826 1332 -6105 -3252 -3066 -5790 -5936 -5924 -6111 -1534 -163 3250 -5537 -5090 1374\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 247 -498 -541 -413 -1484 -3956 -3156 401 -3703 1212 -3654 1398 -152 2063 597 -1904 1408 443 -1365 -3827 -3148 1375\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 248 -947 -4004 -4998 -3847 -4117 -4777 -2971 -3569 2314 1085 376 -3706 -4749 -2693 2806 -3988 -3599 -1005 -4150 -3911 1376\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 249 -303 -3677 521 489 -3998 -3169 -1832 -3749 1863 -3692 -2766 2282 -3265 795 977 528 -2142 -3299 -3859 -3176 1377\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 250 -976 -3658 -684 1157 -3979 -3160 452 -3729 3047 -1448 -646 -1796 -3253 -36 537 -2067 -521 -3280 -3840 -3159 1378\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 251 -5643 -4990 -8015 -7410 1520 -7865 -6421 -461 -7225 3013 823 -7605 -6737 -5858 -6636 -7282 -5461 -812 -4821 -5092 1379\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 252 -5701 -6012 -5073 -4798 -7000 -5671 -4343 -6867 -472 -6451 -5896 -4813 -5919 4537 -3132 -5613 -5551 -6567 -5932 -6008 1380\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -47 -9968 -5007 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 253 -6169 -5697 -6957 -6748 -4382 -6516 -5904 -4040 -769 3340 -3312 -6646 -6623 -5808 -5311 -6821 -6144 -4812 -5585 -5688 1381\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 254 -1716 1095 -6459 -6028 -3940 -6048 -5461 1143 -5841 1305 -2799 -5704 -5919 -5642 -5835 -5289 -824 3032 -5131 -4703 1382\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 255 -52 905 -6348 -6700 -6341 3667 -5673 -6179 -6354 -6438 -5470 -4751 -4720 -5786 -5948 -3282 -3505 -4850 -6519 -6546 1383\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 256 670 948 -4999 -4383 -2449 -4223 -3141 1345 -3995 579 -1660 -3878 -4268 -3630 -3809 503 48 2429 -3000 -2652 1384\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 257 398 -3530 -6269 -6559 -6138 -1036 -5540 -5917 -6198 -6201 -5255 -4650 -4637 -5644 -5824 1839 3162 305 -6382 -6339 1385\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 258 3256 2364 -6346 -6691 -6266 -3827 -5615 -6085 -6299 -6352 -5376 -4676 -4645 -5721 -5888 925 -704 -4758 -6491 -6482 1386\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 259 -5254 -4703 -7615 -7003 -461 -7276 -5909 223 -6758 2682 3305 -6979 -6449 -5604 -6266 -6571 -5100 -1069 -4611 -92 1387\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 260 -5693 -5015 -7996 -7426 3362 -7871 -6113 -2464 -7236 2405 1357 -7535 -6725 -5827 -6620 -7302 -5502 -3375 -4620 -4601 1388\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 261 -4707 -4154 -7316 -6911 -3524 -7176 -6617 3576 -6834 930 421 -6854 -6590 -6165 -6687 -6571 -4653 491 -5369 -5350 1389\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 262 3637 -3624 -6153 -6508 -6314 -3898 -5626 -6170 -6284 -6424 -5461 -4710 -4711 -5726 -5913 -1033 -3500 -4845 -6502 -6494 1390\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 263 1775 3935 -6337 -6686 -6276 -3832 -5620 -6101 -6302 -6367 -5390 -4680 -4650 -5726 -5892 2664 -3421 -4768 -6498 -6489 1391\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 98 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -53 -4813 -10965 -167 -3197 -1363 -710 * * \n";
print INPUT_HMMS " 264 -7582 -6594 -7264 -7208 -7946 -6530 -6425 -8664 4064 -8079 -7834 -7153 -6917 -6542 -5685 -7844 -7590 -8358 -6696 -7636 1393\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 265 -6603 -5514 -6963 -7325 2963 -6834 -3041 -5494 -6887 -4794 -4893 -5465 -6698 -5607 -6246 -6106 -6455 -5652 -2287 4372 1394\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 266 -4890 -6630 455 3830 -6967 -4528 -4056 -7090 -4545 -6872 -6385 -3186 -5125 -3779 -5550 -4496 -5006 -6523 -6780 -5927 1395\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 267 -5015 -6628 -423 3886 -7042 -4638 -4185 -7199 -4675 -6969 -6494 -3326 -5231 -3916 -5653 -4631 -5133 -6635 -6785 -6037 1396\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 268 -2526 -2352 -4823 -4183 -2331 -4082 159 2471 -3689 -2177 2681 -3702 -4128 -3411 1313 -1064 -2468 1974 -2852 -2507 1397\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 269 -959 1399 -2698 -2140 -50 -949 1518 -2450 -549 -2701 1286 1228 -3511 -161 -2394 725 -2185 -2277 2549 3005 1398\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 270 -199 3021 -4721 -4101 -2241 -3916 -2856 -1777 -3709 403 -1488 -3590 2689 -3341 -3527 714 -1025 859 -2743 -392 1399\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 271 -7768 -6613 -7570 -7954 -8124 -6589 -7245 -9201 -8154 -8558 -8496 -7885 4337 -8027 -7600 -8219 -8016 -8763 -6863 -8119 1400\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 272 -2145 1940 482 1019 -3898 -3123 -1783 -1292 780 -1405 -190 32 -3216 632 -566 1203 919 -31 -3779 -168 1401\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -402 -2040 * * \n";
print INPUT_HMMS " 273 -4343 -3816 -7037 -6723 -4310 -6920 -6972 2782 -6709 385 -2994 -6576 -6594 -6596 -6864 -6320 -4330 2834 -6158 -5721 1402\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 274 142 -3653 1965 1164 -3974 121 238 -3724 1031 -1548 -2742 894 -3246 686 -1900 -306 -602 -3275 -3836 -92 1403\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 275 -4532 -6672 3714 634 -6746 -4216 1283 -6770 -4168 -6585 -6061 1448 -4822 -3390 -5239 -4116 -4643 -6194 -6792 -5608 1404\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 276 -6527 -5494 -7056 -7363 3915 -6935 -3165 -4879 -6935 1540 -4215 -5577 -6735 -5647 -6298 -6192 -6372 -5293 -2402 1582 1405\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 277 1493 947 -4976 387 -2425 -4211 -3107 605 -3964 315 301 -3854 -4251 -3598 -3777 -3304 -1367 2507 -2967 -2621 1406\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 278 -2422 722 -1045 -4124 1567 -3969 466 -238 -3721 -6 -128 -3611 -4019 -3345 -3523 -1154 -2363 -710 -2702 3978 1407\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 279 -4221 -3732 -6864 -281 -4123 -6626 -6311 3206 -6401 -1121 2259 -6278 -6366 -6210 -6462 -5951 -4202 1914 -5706 -5312 1408\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 280 159 3175 -5742 -5495 -4001 -3906 -4338 -3493 -5111 -451 -3219 -4349 -4501 -4684 -4870 1307 2961 -790 -4465 -4174 1409\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 281 -4532 -6666 3788 1417 -6751 -964 -3693 -6772 -4170 -6587 -6063 -2796 -4823 -211 -5241 -4117 -4644 -6195 -6794 -5612 1410\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 282 -3887 -5591 1997 -2292 -5865 1765 1695 -5687 625 -5551 -4794 2864 -4487 -2816 -1534 -3611 -3895 -5207 -5693 -4866 1411\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 283 2622 632 -2539 -445 -3112 -1120 -2102 -685 -1871 -2932 347 -574 -3476 -1795 -445 -953 1637 -2511 -3352 -2842 1412\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 284 -349 1668 -6967 -7290 2201 -6810 -3062 -5413 -6847 -4773 -4835 -5465 -6682 -5599 -6226 -6053 -6332 -5549 1600 4333 1413\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 285 -2245 -3720 -492 -441 -4038 -3196 -1871 -741 105 -3734 -2811 1114 -3301 473 -1971 1826 2544 -3340 -3903 -3217 1414\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 286 -1238 -3669 -2065 134 -3990 -868 368 -3735 2087 -3682 -2760 -1822 -3276 -1379 2380 632 -1214 689 -3850 -3176 1415\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 287 17 -3653 1323 1417 -3975 -3154 395 -3725 928 -3670 -2743 817 -3247 748 637 1134 -199 -3276 -3837 -3154 1416\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 288 -4516 -6638 2287 2213 -6727 -1075 -3682 -6742 -4144 -6560 -6027 -2792 -4815 3265 -5199 -4104 -4625 -6168 -6766 -5594 1417\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 289 -4519 -3984 -7172 -6814 -3887 -7036 -6760 3304 -6764 388 1328 -6700 -6596 -6366 -6766 -6427 -4488 1860 -5716 -5523 1418\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 290 -2611 1849 -4939 -4302 -2342 -4174 -3044 713 403 1939 993 -3808 -4209 -3517 1945 -3264 -2551 572 -2903 -2570 1419\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 291 800 -3654 690 1746 -3975 -866 -1813 -3726 -331 -3670 -2743 229 -3248 1120 311 864 -2120 640 -3837 -3155 1420\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 292 1494 -3600 -6127 -6266 -4965 -248 -5299 -4468 -5903 -1479 4819 -4734 -4774 -5440 -5603 -3387 -3513 -4121 -5678 -5517 1421\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 293 -5570 -6508 -3357 3901 -7293 -5190 -4654 -7464 -840 -7148 -6681 -4067 -5718 -4404 -4900 -5274 -5645 -6982 -6627 -6404 1422\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 294 -798 -3194 -2497 -1932 -3330 -3406 -2077 772 2031 -1378 2799 -2174 -3488 1030 1869 -889 -796 -2714 -3494 -2965 1423\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 295 -854 -3300 -440 82 1278 -3261 -1940 -491 165 43 -143 -503 -3349 -459 -2072 555 1686 -2846 -3573 2068 1424\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 296 -4735 -4185 -7344 -6939 -3574 -7198 -6641 3324 -6859 -232 3442 -6875 -6623 -6203 -6716 -6589 -4682 205 -5411 -5384 1425\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 297 -765 -4926 -7919 -7332 -166 -7686 -6274 -589 -7132 3202 -1734 -7432 -6680 -5825 -6572 -7073 -5380 -3253 -4771 -4951 1426\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 298 -795 -3652 -529 -482 -3973 203 243 -3724 1282 -1415 1343 1849 -3246 1026 1017 704 -602 -3274 -3836 -3153 1427\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 299 593 -3612 -2048 444 -3915 -514 -1825 -3652 131 -1456 -2706 426 -3257 -53 -1917 261 2129 1300 -3807 -3135 1428\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 300 -5385 -4765 -7848 -7317 -3093 -7707 -6528 -499 -7170 3221 -1849 -7440 -6731 -5963 -6693 -7133 -5252 54 -4967 -5185 1429\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 301 -3754 -5507 635 1869 -5736 404 -3136 -5589 1051 -5483 -4699 2649 -4395 1951 -3843 -3484 -3772 -5093 -5664 -4775 1430\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 302 -6632 523 -7000 -7361 4170 -6882 -3061 -5516 -6916 -4819 -4917 -5489 -6736 -5631 -6275 -6131 -6480 -5673 3184 1799 1431\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 303 -1608 -4129 2705 1067 -4435 -1144 -2196 -4202 -810 -4139 -3233 2070 -3592 740 -2392 -1515 -2563 -3745 -4309 1665 1432\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 304 -5671 -5008 -8042 -7440 598 -7912 -6450 2738 -7260 2456 266 -7652 -6756 -5875 -6663 -7342 -5486 -3308 -4828 -5095 1433\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 305 -959 470 -6206 -6453 -6269 2471 -5559 -6087 -6146 -6342 -5372 2027 -4677 -5622 -5830 1756 1026 -4785 -6487 -6448 1434\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 306 -2420 1575 -4764 -4128 -321 -3968 -2838 1663 -3724 424 -1449 -3613 -4017 -3346 1725 -127 -2361 505 3453 1350 1435\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 307 -1258 -4695 -6561 -6924 -7076 -4906 -6303 -7198 -6970 -7280 -6506 -5717 4274 -6544 -6617 -4502 -4707 -6001 -6722 -7163 1436\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 308 -2455 -2339 -4605 -4000 -2329 741 -2887 559 -3645 1208 -1570 1520 -4036 -3309 -3523 185 2082 -32 -2828 -2481 1437\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 309 558 -3556 -3486 -3172 -4498 -3756 312 -728 -3059 -4341 -3549 -3195 3550 1324 -3385 323 -3003 -3719 -4677 -4211 1438\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 310 -4137 -3820 -6430 -5813 -2673 -5800 -4436 -2304 -852 1875 2850 -5409 -5564 -4778 -5070 -4945 -4044 -585 -3848 3538 1439\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 311 -2274 -3610 -2172 -1624 -3901 -3256 2812 -3613 -1482 -3613 -2725 2135 422 -607 1311 427 -505 1502 -3823 -3183 1440\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 312 -6659 -5575 -7018 -7379 4519 -6867 -3123 -5561 -6950 -4859 -4959 -5541 -6748 -5682 -6312 -6177 -6518 -5722 -2370 526 1441\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 313 -5711 -5039 -8079 -7473 -2955 -7961 -6491 -558 -7295 3254 1086 -7712 -6774 -5885 -6683 -7405 -5521 -3352 -4838 -5125 1442\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 314 -424 -4918 -5666 -4145 -441 -5065 -3104 -5029 459 -4882 -4243 -3932 -5070 -2718 3898 -4431 -4203 -1205 -4835 -4696 1443\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 315 -4832 1983 -6163 -4275 -5711 -5318 -3085 -5367 -325 -1322 -4435 -4029 -5219 -2695 3956 -4750 -4461 -5208 -4835 -1046 1444\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 316 1288 -3632 -6060 -5594 2787 -5433 -3468 2314 -5208 -339 -2298 -4881 -5363 -4611 -4927 -4562 -3867 -2723 -2908 2101 1445\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 317 1031 -2470 -4874 -4299 -2548 -1034 -3121 656 -3924 54 -1787 -3752 -4145 -3565 -3763 2678 327 -625 -3050 -2709 1446\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 318 -4870 -5423 -5993 -4182 -6408 -5309 -3062 -683 3591 -5199 -4558 -3973 -5200 -211 1610 -1232 -4474 -5416 -4996 -4975 1447\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 319 2786 3328 -5805 -5457 -3718 -4242 -4327 684 -5093 -3400 -2860 -4520 -4707 -4703 -4882 -1576 -739 926 -4320 -3994 1448\n";
print INPUT_HMMS " - -147 -500 235 45 -381 398 105 -627 210 -466 -721 275 393 48 95 359 117 -370 -295 -250 \n";
print INPUT_HMMS " - -3177 -4858 -226 -1885 -455 -701 -1378 * * \n";
print INPUT_HMMS " 320 1054 -1106 -3265 -2718 2155 -2778 -1584 46 -2398 1935 413 -2359 -2767 -1974 -2279 -1907 -1227 -174 -1274 -820 1453\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -15 -7187 -8230 -894 -1115 -33 -5487 * * \n";
print INPUT_HMMS " 321 1036 1842 1956 -439 -3873 959 -1835 -217 -254 -3586 -2681 -84 -3265 -1386 -1930 576 -604 -321 -3786 -3122 1454\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 322 -2179 -3653 1678 1378 -590 -323 2024 -134 367 -3669 -2742 748 -795 1152 -343 78 -2118 -3275 -3836 -3153 1455\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 323 1453 720 -4578 -3968 -2302 -3929 -2855 -1844 -3612 -2197 -1547 175 -4013 -3277 -3492 1260 -828 1261 1416 2881 1456\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 324 -3004 703 3513 254 -4870 -1222 -2545 -4656 -2299 -4586 -3709 -520 -3895 -27 -2876 236 -63 -4188 -4761 -4004 1457\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 325 1315 -2468 -3557 -1005 -344 -3733 1323 1117 522 -980 2862 -2946 172 -416 -2956 -323 -2299 229 -2896 -2508 1458\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 326 -2346 -3835 709 2316 -4151 -1491 -1962 -3907 1853 -3849 -2929 -277 -3383 2073 -2086 -1003 -2290 -479 -4017 -3323 1459\n";
print INPUT_HMMS " - -148 -494 235 43 -376 397 104 -622 210 -468 -722 275 395 46 94 357 116 -371 -296 -239 \n";
print INPUT_HMMS " - -82 -4184 -11010 -3059 -184 -701 -1378 * * \n";
print INPUT_HMMS " 327 -2388 405 -4027 -3431 -2323 -3842 508 336 -1311 509 1247 -567 -809 1632 -3206 -343 2252 564 -2797 -2433 1469\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 328 -4291 -4942 -4431 1657 -4244 -4925 3240 -4901 -1835 1189 -4081 -3618 -4930 -2663 2525 -4210 -4061 -4718 -4240 -322 1470\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 329 -2406 -2288 -4424 -3809 -114 -3915 415 596 -999 -1200 617 1728 -3969 -3156 -3388 -1750 3058 -733 -2740 -2389 1471\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 330 -5487 -4854 -7913 -7348 -3030 -7773 -6474 680 -7185 2908 2428 -7502 -6730 -5910 -6661 -7190 -5333 17 -4899 -5146 1472\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 331 2779 984 -5078 -4636 -3336 401 -3687 -463 -4316 -3234 -2562 -3969 -4316 -370 -4224 1063 -2857 421 -3805 -3475 1473\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 332 -4288 922 -4900 -3684 774 -4936 1283 -5133 3242 -4881 -4187 -3630 -4893 -2512 1931 -657 -4025 -4894 -4761 -4549 1474\n";
print INPUT_HMMS " - -149 -500 233 43 -381 398 105 -627 212 -464 -721 277 393 45 96 359 117 -370 -295 -250 \n";
print INPUT_HMMS " - -47 -4983 -11010 -1498 -631 -701 -1378 * * \n";
print INPUT_HMMS " 333 -6572 1653 -6985 -7335 3103 -6851 -3065 -5491 -6882 -4806 -4896 -5482 -6717 -382 -6254 -6102 -6434 -5644 -2312 4096 1478\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 334 -1283 -4805 -7759 -7155 1172 -7476 -6119 2123 -6931 2589 1082 -7191 -6572 -5726 -6419 -6804 -5226 -3186 -4730 -4929 1479\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 335 188 2769 -4745 -4111 -2201 -414 -2834 952 -3710 1175 3111 -3604 -4014 -340 -3516 -163 -636 -492 -2705 -2362 1480\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 336 -5570 -6508 -3357 3901 -7293 -5190 -4654 -7464 -840 -7148 -6681 -4067 -5718 -4404 -4900 -5274 -5645 -6982 -6627 -6404 1481\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 337 -2549 -2366 -4897 -4264 -2284 -4109 -2983 1032 -3863 2446 1238 -3755 -4148 -3479 -3663 -1126 661 294 -2832 858 1482\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 338 767 1331 -3761 -3230 -3134 635 -2939 -2706 -773 -3009 -2309 -3158 -3979 -2890 -3287 1660 2499 -674 -3531 -77 1483\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 339 -3695 -3421 -6056 -5434 -2700 -5359 -4207 1211 -5069 2747 2195 -5015 -5219 -4514 -4815 -1192 -3617 -782 -3766 -78 1484\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 340 -2565 1713 -4914 -4281 588 -4126 -3001 73 -3880 1071 2789 -3773 -4164 1569 -3679 -3214 -1120 2016 -2847 -2514 1485\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 341 -2989 1682 3207 1864 -4813 -3630 1245 -4589 -2281 -4533 -3661 -2251 -3885 -216 -2852 -931 -2959 -983 -4718 -3970 1486\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 342 -2310 -2736 -2921 -678 364 -3542 2514 117 -2219 -2622 284 -443 -793 -7 -2562 -303 -2250 -2197 -3114 3761 1487\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 343 -2180 -3652 2321 1549 -3973 -3153 248 -897 991 -3668 -2741 -1789 -795 707 803 -802 -490 -345 -3836 -3153 1488\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 344 -2428 947 -4774 -4138 2540 -3976 -2847 -461 -3733 403 3667 -3622 -4026 -3355 -3533 -1156 -720 -465 -2711 1681 1489\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 345 -349 -3081 -5844 -5297 -3250 -5213 -4268 1880 -4980 977 341 -4863 -5182 -4658 -4848 -174 -3343 2697 -4054 -3693 1490\n";
print INPUT_HMMS " - -149 -500 233 43 -381 398 105 -623 210 -466 -721 275 394 45 96 359 117 -367 -295 -249 \n";
print INPUT_HMMS " - -84 -4149 -11010 -109 -3781 -701 -1378 * * \n";
print INPUT_HMMS " 346 -903 -3649 -2029 50 -3968 595 3251 -3717 -240 -1611 1680 -1790 63 1236 539 687 -603 -3270 -3833 -3151 1492\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 347 282 -2268 -4563 560 1976 -3937 -2798 167 -970 164 -1470 -676 -3989 -3236 -3445 -3013 -830 798 -2723 2884 1493\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 348 -904 497 -2046 -1497 -3934 -3164 2105 -3674 180 83 -2719 -3 2095 -32 1131 1220 -858 -3241 -3817 -3143 1494\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 349 1073 -3022 -3335 -2764 -3224 -3721 -2617 -647 -2375 -3060 391 -2840 3504 -148 125 -936 -2558 -2616 -3530 -3107 1495\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 350 -4003 -4530 -5370 -5716 -5756 -4677 478 -6855 -6025 -6860 -6141 -5082 -5412 -5738 -5947 3711 -4447 -5734 -5973 -5455 1496\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 351 -1185 -3311 -2241 312 -430 -3258 1183 -3159 1635 833 1636 -1967 -3347 1993 -389 -2181 -607 -2859 1966 -149 1497\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 352 -2472 1592 -4819 -4186 -2247 -4028 -2903 1739 -3783 2171 1520 -3673 -4074 -440 -3585 -1041 -1023 861 -2765 -2423 1498\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 353 3408 844 -6263 -6367 -5265 -3976 -5299 -770 -5991 -5010 -4368 -4705 -4723 -5494 -5666 -64 -3418 -676 -5752 -5553 1499\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 354 3435 -3592 -6314 -6658 -6290 -3878 -5645 -6032 -6315 -6362 -5406 -4713 -4694 -5747 -5920 825 -3465 -1000 -6524 -6500 1500\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 355 2540 -3516 -4789 -4468 -5281 510 -4316 -4981 -863 -5198 1318 -3968 -4448 -4119 -4495 1668 1000 -4227 -5492 -5160 1501\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 356 3149 2712 -6384 -6733 -6322 -3876 -5665 -6146 -6348 -6412 -5435 -4724 -4694 -5771 -5937 1438 -3465 -4812 -6543 -6535 1502\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 357 -730 -2248 -891 -4129 1608 -3969 -2840 480 -3724 944 3131 -3614 -4018 -3347 -3525 -396 -2362 1065 -2706 1591 1503\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 358 -1520 3213 -4781 -4146 1945 -3985 -2845 646 -3741 -98 -1462 -3628 -4034 -395 -3541 -3070 -2378 -421 1127 3317 1504\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 359 -411 -3552 -6199 -5575 -233 -5515 -4355 1323 -5215 2570 478 -5175 -5339 -4622 -4947 -4644 1347 -2494 -3859 -3699 1505\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 360 2886 899 -6387 -6726 -6309 -763 -5655 -6131 -6336 -6394 -5418 -4718 -4688 -5759 -5929 1993 259 -4802 -6533 -6523 1506\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 361 -4137 -4487 -5180 -3911 -483 -4976 -3020 -4067 300 1311 1769 -3787 -4915 2535 2608 -4233 -3903 -4059 -4449 -4253 1507\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 362 -2196 2228 -2120 62 -402 -3200 -1867 -977 2443 -1900 -2594 -287 -3291 686 793 283 -639 -1138 -3714 599 1508\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 363 -2550 684 -4906 -4278 -221 -4123 -3008 2134 -3880 -1001 3573 -3769 -4166 -3508 -3686 -1185 958 1348 -2868 -2524 1509\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3008 -9968 -193 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 364 -1150 -930 -3207 -2657 -683 -2825 -1821 2150 -2347 1483 321 -2380 -2789 -2031 -2296 -1958 1565 642 -1637 -1343 1510\n";
print INPUT_HMMS " - -149 -500 235 43 -381 398 105 -627 210 -466 -721 277 393 45 98 359 117 -370 -295 -250 \n";
print INPUT_HMMS " - -2141 -378 -8018 -67 -4472 -32 -5518 * * \n";
print INPUT_HMMS " 365 -2651 -2880 -3618 -2963 -1182 -3913 165 -31 1602 2453 -2061 -471 -595 -2457 258 -2950 -2572 -2339 -3246 -2861 1512\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 366 -1224 -3653 807 -1478 -3974 1672 140 -3725 1383 -1516 -2742 2226 -965 181 239 -1286 -1170 -3275 -3836 -3153 1513\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 367 -2181 1180 -2037 285 -3945 516 1082 -1429 1217 288 -2724 476 -3251 1773 283 -358 -227 -341 -3821 -3144 1514\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 368 -2182 -3625 445 326 -45 2183 604 -3676 -1406 -1460 -2718 1564 -3253 516 -1911 -272 38 -1147 -3816 -530 1515\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 369 -2179 -3653 499 1338 -610 462 -1812 -3724 869 -3669 -2742 570 1608 567 -683 72 -127 -3275 -3836 664 1516\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -1506 -9968 -628 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 370 -1094 -2252 302 1530 510 -2123 -798 120 1210 -340 -1373 -818 -2213 1050 -920 -1043 427 178 -2510 -1905 1517\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -6 -8468 -9510 -894 -1115 -42 -5111 * * \n";
print INPUT_HMMS " 371 -3456 813 -6422 -6408 -3732 -4482 -4770 31 -5882 -3943 -3738 -5008 -5117 -5482 -5606 -3835 -760 -3146 6058 -3678 1518\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 372 -913 -3901 953 -641 -4255 -838 -2099 -4008 -1730 -3964 -3056 2341 -3492 -1654 -2251 1170 2200 -332 -4144 -3452 1519\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 373 242 -3653 170 158 -3974 178 -1812 -3724 6 -104 -2742 -76 2397 698 -429 366 -1044 -3275 -3836 -3153 1520\n";
print INPUT_HMMS " - -145 -501 232 44 -381 398 105 -627 211 -467 -721 276 393 44 95 358 118 -368 -295 -250 \n";
print INPUT_HMMS " - -82 -4184 -11010 -1926 -441 -701 -1378 * * \n";
print INPUT_HMMS " 374 -200 1650 -3564 -2986 -520 -3733 -2539 -2027 857 -2350 620 791 -3804 -2599 -2966 -336 2917 -92 -2912 -2525 1525\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 375 -2440 1860 -4746 -4113 -230 -3984 1853 -1753 -3716 2467 993 -3617 -4032 1116 -3529 -398 -2381 -654 -2725 -2382 1526\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 376 -2334 -3626 -2230 2029 -3894 -3314 591 1085 608 -3607 -2734 -1978 -489 2308 -1961 -2246 -2268 1267 -3830 -3207 1527\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 377 -2748 409 -2815 130 2285 -3731 3870 -3665 -332 -3709 -2914 -2434 -3799 83 -391 -2702 -2661 -3377 -3762 2450 1528\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 378 -5560 -4966 -6493 -6426 32 -6338 3332 -619 -146 -4438 -4274 -5178 -6264 -5142 -5471 -1248 -5455 -4794 -2351 4208 1529\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 379 -2602 1338 -4591 -4063 -2901 -3891 -3237 -2447 -3735 -784 1369 -3661 -4168 390 -3725 2584 2294 -2313 -3378 -3030 1530\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2144 -9968 -372 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 380 -1882 -1908 -3561 -3291 929 2968 -1977 -1131 -3039 1063 -739 -2801 -3305 -2664 -2957 -2313 -1974 -1292 -1628 -822 1531\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -9 -7833 -8875 -894 -1115 -36 -5350 * * \n";
print INPUT_HMMS " 381 -897 -3935 -3117 -2971 -5331 3390 974 -5090 -621 -5118 -4236 -481 -4253 -3014 -3504 -201 -1187 -4386 -5291 -4728 1532\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 382 -476 -4861 -6584 -6605 137 -6312 473 75 -6198 -4358 -4169 -546 -6248 -5259 -5757 -5503 -5374 -4650 -2339 4567 1533\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -47 -9968 -5007 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 383 -1238 -3626 1074 218 -3947 -3123 -1784 -3698 634 -3642 -734 336 -3218 513 -1874 1756 2159 -3248 -3810 -3126 1534\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 384 331 -3590 -2003 2620 -3899 -950 -1783 -178 955 -3601 278 -1763 -688 519 -1873 -204 -748 -3206 -3780 103 1535\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 385 550 -3615 1083 1577 -3936 -3116 499 -947 490 -3631 539 270 -3209 717 -321 830 -2 -3237 -3799 -151 1536\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 386 -670 -3687 2017 1683 -4006 -3159 1127 -3758 -384 -3702 -2778 -1792 -3264 2459 -1936 689 -2149 -1255 -3869 -3182 1537\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 387 -4578 -4060 140 -6723 -3517 -6932 -6309 1686 -6621 2788 -2275 -6602 -6453 -6019 -6486 -6280 -4529 472 -5260 -5181 1538\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 388 51 -2264 -4277 -251 -423 -3858 1197 978 402 1008 2644 -3355 -725 358 -3303 -2923 -2303 255 -2714 835 1539\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 389 204 -3032 966 1536 -3146 -3321 -2021 -763 -1761 -1024 -204 -2109 2623 -1691 -2198 361 -2154 -701 -3357 -2831 1540\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -1363 -710 * * \n";
print INPUT_HMMS " 390 -266 3489 -4724 -4088 614 -3927 338 115 -3683 1239 -1409 -3573 -1209 -3306 -3483 -3012 -1072 1871 -2664 274 1541\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9923 -10965 -894 -1115 -402 -2040 * * \n";
print INPUT_HMMS " 391 1480 2514 -4609 -3985 -2233 -3950 -2818 -420 -949 -2124 2888 -3546 -4004 -419 -3470 -18 -2363 2039 -2733 -2387 1542\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 392 -624 -3652 -495 244 -3973 -952 1392 -897 1771 -3668 -2741 -1789 -3246 1888 1576 111 -637 -1081 -3835 -188 1543\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 393 -890 -2414 -3902 -3303 -280 -3832 3002 1092 1229 1338 1240 -3162 -3891 375 387 -2875 -2345 -1840 -2850 -2481 1544\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 394 -5142 775 -7510 -6900 -2916 -7118 -5807 2022 -6640 2129 3551 -6815 -6393 -5569 -6186 -6383 -5001 -3118 1245 -4738 1545\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 395 1861 -2273 -4527 -3906 -2231 -3932 1302 256 -3550 459 2197 1694 -3984 -3216 -717 -1051 -2351 581 -2727 -2380 1546\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 396 -1102 -3653 1059 1398 682 -1385 2211 -3725 2094 -3669 -2742 -269 -3246 680 -683 -1019 -2119 -3275 -3836 -290 1547\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -384 -9968 -2105 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 397 -2094 2212 -4397 -3764 -1880 -3637 -2506 -11 -3368 1127 1597 2482 -3687 -210 -3183 -2720 -278 -444 -2384 2043 1548\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9587 -10629 -894 -1115 -1720 -522 * * \n";
print INPUT_HMMS " 398 -2460 1916 -4814 -4190 -2188 -4048 608 684 -3794 2008 -1380 -3693 -4077 -306 -3602 -3142 -2401 2286 -2776 -2441 1549\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -645 -9685 -1477 -894 -1115 -132 -3510 * * \n";
print INPUT_HMMS " 399 -486 -1899 -3760 -599 -130 -3448 919 588 -472 922 -1098 -171 -3504 -2587 -2857 -820 1460 1567 1451 -1988 1550\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9464 -10506 -894 -1115 -3425 -141 * * \n";
print INPUT_HMMS " 400 -1824 -3099 621 -1203 1287 -2827 2868 -3046 1833 -1819 856 -1496 -2917 56 316 -1744 -1762 -2683 -3311 2008 1551\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -106 -9464 -3842 -894 -1115 -2565 -267 * * \n";
print INPUT_HMMS " 401 -1778 -2874 -172 -1250 -3061 -2818 1391 413 568 -122 -2001 -1527 -2907 1314 -42 1026 -1717 1809 -3143 -2554 1552\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9417 -10459 -894 -1115 -984 -1017 * * \n";
print INPUT_HMMS " 402 10 -3464 -1843 -66 -3787 -2967 -1621 -3535 1783 -3478 -2554 2462 -3060 134 328 1346 -1934 -768 -3643 -2965 1553\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9712 -10754 -894 -1115 -142 -3411 * * \n";
print INPUT_HMMS " 403 850 -3652 942 799 213 -1572 452 -3723 -788 -3668 -2741 1009 -64 1375 41 -129 -2118 229 -3835 572 1554\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -82 -9968 -4203 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 404 859 -3618 -1983 -428 -3944 1519 -1776 -3693 1064 -3636 -2711 1096 1165 -1317 1168 346 -2089 -3244 -3801 -3121 1555\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9888 -10930 -894 -1115 -1718 -523 * * \n";
print INPUT_HMMS " 405 -2114 -3585 721 635 -263 -175 1634 -3656 -265 458 -2674 -381 1132 453 -1031 340 662 -3207 1214 -3086 1556\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9888 -10930 -894 -1115 -304 -2395 * * \n";
print INPUT_HMMS " 406 -795 -3648 937 -563 -3967 279 -1813 -1089 -359 -1552 -2738 569 -3247 4 -458 1148 2199 -1062 -3833 -3151 1557\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 407 -2192 -3660 -2042 139 -3981 -3166 1963 -161 2883 -3674 -2750 306 -3258 737 251 -948 -492 -1155 -3842 -3162 1558\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 408 -4577 -4197 -6180 -5962 1099 -1155 3857 -3829 -5560 2332 -3444 -4907 -5728 -4851 -5231 -1332 -4510 -3813 -2393 -89 1559\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 409 -976 623 1014 228 -3809 -3184 421 77 1388 -215 192 474 -3276 -1406 -520 -154 1162 -594 -3752 73 1560\n";
print INPUT_HMMS " - -149 -500 233 43 -381 400 105 -627 212 -466 -721 275 393 45 96 359 119 -370 -295 -250 \n";
print INPUT_HMMS " - -42 -5144 -11010 -1489 -636 -701 -1378 * * \n";
print INPUT_HMMS " 410 2446 -3300 -2501 -4 -3610 639 -2187 -3262 -1878 -1410 -2585 -372 -3514 -1813 -2336 1068 442 -592 -3737 -3175 1564\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 411 -4089 -3638 -6684 -6268 1424 -6336 -5758 2302 -6108 17 -2750 -1688 -6135 -5857 -6098 -5604 -4063 2873 -5272 -4872 1565\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 412 -2597 -2562 -4191 -3592 1749 -4025 1948 -528 1297 -508 -1761 -3379 -4078 -3036 2312 -1024 -2534 -1995 2066 1754 1566\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 413 -2184 -3655 -2031 178 -3976 -1299 -1815 -3726 1586 -723 -2744 2043 -3250 738 1847 223 0 -3277 -3838 -424 1567\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 414 -4759 -6029 -512 -3024 -6659 -4662 -3532 -6304 3929 -5973 -5382 -3304 -5066 -1142 -2840 -4453 -4685 -5936 -5852 -5420 1568\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 415 -6656 -5569 -7013 -7375 489 -6871 -3108 -5557 -6942 -4857 -4957 -5528 -6746 -5670 -6304 -6167 -6512 -5715 -2355 4852 1569\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9968 -11010 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 416 1380 -3745 -1097 -1553 -620 -3225 -1912 -3815 737 -3766 -2847 143 -3337 1395 -2013 2492 -2226 -3370 -3938 -3255 1570\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 417 -2192 -3665 -509 231 -3985 -3157 2661 -3736 994 -3680 -2755 -1792 -3254 122 -1912 2331 955 -1108 -3848 -3165 1571\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 418 -2162 669 -559 241 -3956 -3136 622 -3707 952 -1626 -2725 -179 1102 -10 1012 2153 -1197 -3257 -3819 -3136 1572\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 419 -2379 -3482 -2408 49 -3699 -1190 -2025 -149 2687 -3435 -2607 -2098 -3478 -1621 2009 -2335 -640 624 -3715 117 1573\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 420 -2406 -2596 -3406 -825 3234 -3730 1830 -2158 -2607 579 563 -555 -3796 -164 92 -2740 -2345 -2040 -2923 1808 1574\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 421 170 458 -4665 -4035 2103 1277 644 -1731 71 945 2510 -3558 -3989 -3285 -3475 -3019 -2340 -1652 -2692 840 1575\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 422 -273 792 -2015 1287 -3943 -850 1769 -3690 2079 -1496 274 -1776 -3232 591 1018 -768 -2103 -3246 -3812 894 1576\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 423 1052 765 -6517 -6172 -4181 -5656 -5635 2289 -5987 -3235 -3024 -5646 -5800 -5778 -5965 -1283 -3857 2869 -5386 -4955 1577\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 424 2413 -3697 -4964 -5026 -5515 -3929 -4730 -5663 -854 -5833 -4989 -4276 -4662 -4657 -4619 2715 -3488 -4676 -5766 907 1578\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 119 -369 -294 -249 \n";
print INPUT_HMMS " - -35 -5412 -10989 -230 -2765 -701 -1378 * * \n";
print INPUT_HMMS " 425 -818 159 -3231 -2661 521 -1094 -2397 588 1457 729 356 -700 -3698 519 592 -859 1258 -2000 -2968 -35 1580\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 426 -2405 446 -4743 -4107 1657 -3952 -2823 2666 -947 938 699 -3595 -4002 626 -3506 -3036 445 -1644 -2689 -310 1581\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 427 -455 -3132 -2405 563 -3284 -3325 -2038 -2913 -441 -3085 1109 -2105 2793 -1687 -2198 468 130 1346 -3462 -2920 1582\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 428 398 719 -3078 -817 509 -3580 2226 -671 -2355 -2500 1378 -2612 1466 1850 -611 -987 836 60 -3023 -2598 1583\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 429 860 -3611 1382 139 -3921 -3143 -1803 -3663 712 1664 26 -182 -3236 657 -1894 -668 -2105 -1168 -3801 -3126 1584\n";
print INPUT_HMMS " - -149 -500 234 44 -381 398 105 -627 211 -467 -721 275 397 45 95 360 117 -370 -295 -250 \n";
print INPUT_HMMS " - -287 -2474 -10989 -37 -5288 -701 -1378 * * \n";
print INPUT_HMMS " 430 -928 -3628 1237 276 -3946 -597 957 -966 392 -1534 -279 1441 -3231 3 -996 -780 -2102 -1265 4176 -3133 1586\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 431 66 2291 -988 -4090 1101 -3945 -2815 1135 -964 649 1134 -3584 -3995 -3316 -3497 1250 -524 906 -2686 -2344 1587\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9947 -10989 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 432 132 -3590 -1092 1039 -634 -3148 -1809 -3627 1010 1752 -2685 -1791 -4 2 110 -1081 -585 -608 -3786 -3116 1588\n";
print INPUT_HMMS " - -149 -504 244 41 -381 396 107 -627 208 -466 -724 276 392 44 96 359 117 -371 -298 -244 \n";
print INPUT_HMMS " - -194 -2997 -10989 -2915 -205 -701 -1378 * * \n";
print INPUT_HMMS " 433 -522 -2746 -701 -711 -233 -3497 -2232 447 1920 642 333 -2436 -3574 -185 -2498 306 841 525 -3126 -2673 1607\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9914 -10956 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 434 -153 -2228 -4468 -3848 -64 -1233 -2741 1332 -893 724 590 469 -3935 518 -3379 26 -620 1857 -2682 -2334 1608\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9880 -10922 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 435 -2107 -3580 -687 2124 -3901 -3081 1384 -825 1136 -3596 -2669 -378 -3174 396 769 350 844 -453 -3763 -3081 1609\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -2 -9782 -10824 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 436 -111 685 1689 1676 -3824 -412 578 -3574 800 -3518 -2592 722 -3096 -1201 837 531 -1968 -3124 -3686 -3003 1610\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9734 -10776 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 437 -767 -2098 -4149 -3538 -177 -3702 1098 1491 -3216 1507 1428 974 293 -2912 -585 387 -449 -1515 -2548 -2196 1611\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9538 -10580 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 438 1573 -1962 -3813 -3211 108 893 1544 -1471 -2918 866 1108 -2955 -3564 -28 -2914 453 -144 -194 -2409 -2051 1612\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9484 -10526 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 439 932 -3263 -1640 -59 -3583 -2766 849 -451 651 -3278 -2352 1167 -2860 1389 1123 740 -132 -2884 -3446 995 1613\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -3 -9383 -10425 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 440 146 1926 1089 -1213 -3039 -2784 -1462 -432 -1117 -2808 1016 1638 1122 -1063 786 811 -1684 -2399 -3118 428 1614\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9215 -10257 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 441 779 -1709 691 -2908 -1676 -397 -2075 768 -2623 1665 1145 -2670 -3296 219 -2633 -444 -1709 550 -2155 -1796 1615\n";
print INPUT_HMMS " - -149 -500 233 43 -381 399 106 -626 210 -466 -720 275 394 45 96 359 117 -369 -294 -249 \n";
print INPUT_HMMS " - -4 -9215 -10257 -894 -1115 -701 -1378 * * \n";
print INPUT_HMMS " 442 -856 1044 -2063 -61 185 -288 1011 -889 36 301 2817 167 -28 -1295 -1778 -64 815 -1760 -2638 -2156 1616\n";
print INPUT_HMMS " - * * * * * * * * * * * * * * * * * * * * \n";
print INPUT_HMMS " - * * * * * * * * 0 \n";
close(INPUT_HMMS);
($errorcode,$errormsg) = &run_genewise_on_scaffolds2($outputdir,$input_fasta,$input_hmms,$output,'no','none',$fam_seqs,0.05,25000,1);
if ($errorcode != 0) { print STDERR "ERROR: test_run_genewise_on_scaffolds2: failed test1\n"; exit;}
# WRITE A FILE WITH THE EXPECTED OUTPUT:
$random_number = rand();
$expected_output = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_output") || die "ERROR: test_run_genewise_on_scaffolds2: cannot open $expected_output\n";
print EXPECTED "TF101001\n";
print EXPECTED "V:15395346..15397430 GeneWise match 345 1681 525.17 + . V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise cds 345 396 0.00 + 0 V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise intron 397 448 0.00 + . V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise cds 449 543 0.00 + 2 V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise intron 544 594 0.00 + . V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise cds 595 1017 0.00 + 0 V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise intron 1018 1066 0.00 + . V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise cds 1067 1560 0.00 + 0 V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise intron 1561 1605 0.00 + . V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "V:15395346..15397430 GeneWise cds 1606 1681 0.00 + 1 V:15395346..15397430-TF101001-genewise-prediction-1\n";
print EXPECTED "//\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $output $expected_output |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_run_genewise_on_scaffold2: failed test1 (files $output $expected_output)\n"; exit;}
# DELETE TEMPORARY FILES:
system "rm -f $output";
system "rm -f $expected_output";
system "rm -f $input_hmms";
system "rm -f $fam_seqs";
}
#------------------------------------------------------------------#
# TEST &make_hmmfile
sub test_make_hmmfile
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES TO
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
my $hmmfile; # FILE CONTAINING A HMM
my $input_hmms; # INPUT FILE OF HMMS
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAME
my $expected_hmmfile; # FILE CONTAINING EXPECTED CONTENT OF $hmmfile
my $differences; # DIFFERENCES BETWEEN $hmmfile AND $expected_hmmfile
my $length_differences; # LENGTH OF $differences
my $line; #
# WRITE THE INPUT FILE OF HMMS:
$random_number = rand();
$input_hmms = $outputdir."/tmp".$random_number;
open(INPUT_HMMS,">$input_hmms") || die "ERROR: test_make_hmmfile: cannot open $input_hmms\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF350597.0\n";
print INPUT_HMMS "LENG 1094\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101000.0\n";
print INPUT_HMMS "LENG 1095\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101001.0\n";
print INPUT_HMMS "LENG 1096\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
close(INPUT_HMMS);
($hmmfile,$errorcode,$errormsg) = &make_hmmfile("TF101000",$outputdir,$input_hmms);
if ($errorcode != 0) { print STDERR "ERROR: test_make_hmmfile: failed test1\n"; exit;}
# OPEN A FILE CONTAINING THE EXPECTED CONTENTS OF $hmmfile:
$random_number = rand();
$expected_hmmfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_hmmfile") || die "ERROR: test_make_hmmfile: cannot open $expected_hmmfile\n";
print EXPECTED "HMMER2.0 [2.3.2]\n";
print EXPECTED "NAME TF101000.0\n";
print EXPECTED "LENG 1095\n";
print EXPECTED "ALPH Amino\n";
print EXPECTED "//\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $hmmfile $expected_hmmfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_make_hmmfile: failed test2 (files $hmmfile $expected_hmmfile)\n"; exit;}
system "rm -f $input_hmms";
system "rm -f $hmmfile";
system "rm -f $expected_hmmfile";
# WRITE THE INPUT FILE OF HMMS:
$random_number = rand();
$input_hmms = $outputdir."/tmp".$random_number;
open(INPUT_HMMS,">$input_hmms") || die "ERROR: test_make_hmmfile: cannot open $input_hmms\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF350597.0\n";
print INPUT_HMMS "LENG 1094\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101000.0\n";
print INPUT_HMMS "LENG 1095\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101001.0\n";
print INPUT_HMMS "LENG 1096\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
close(INPUT_HMMS);
($hmmfile,$errorcode,$errormsg) = &make_hmmfile("TF101001",$outputdir,$input_hmms);
if ($errorcode != 0) { print STDERR "ERROR: test_make_hmmfile: failed test2\n"; exit;}
# OPEN A FILE CONTAINING THE EXPECTED CONTENTS OF $hmmfile:
$random_number = rand();
$expected_hmmfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_hmmfile") || die "ERROR: test_make_hmmfile: cannot open $expected_hmmfile\n";
print EXPECTED "HMMER2.0 [2.3.2]\n";
print EXPECTED "NAME TF101001.0\n";
print EXPECTED "LENG 1096\n";
print EXPECTED "ALPH Amino\n";
print EXPECTED "//\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $hmmfile $expected_hmmfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_make_hmmfile: failed test2 (files $hmmfile $expected_hmmfile)\n"; exit;}
system "rm -f $input_hmms";
system "rm -f $hmmfile";
system "rm -f $expected_hmmfile";
# WRITE THE INPUT FILE OF HMMS:
$random_number = rand();
$input_hmms = $outputdir."/tmp".$random_number;
open(INPUT_HMMS,">$input_hmms") || die "ERROR: test_make_hmmfile: cannot open $input_hmms\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF350597.0\n";
print INPUT_HMMS "LENG 1094\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101000.0\n";
print INPUT_HMMS "LENG 1095\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101001.0\n";
print INPUT_HMMS "LENG 1096\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
close(INPUT_HMMS);
($hmmfile,$errorcode,$errormsg) = &make_hmmfile("TF350597",$outputdir,$input_hmms);
if ($errorcode != 0) { print STDERR "ERROR: test_make_hmmfile: failed test3\n"; exit;}
# OPEN A FILE CONTAINING THE EXPECTED CONTENTS OF $hmmfile:
$random_number = rand();
$expected_hmmfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_hmmfile") || die "ERROR: test_make_hmmfile: cannot open $expected_hmmfile\n";
print EXPECTED "HMMER2.0 [2.3.2]\n";
print EXPECTED "NAME TF350597.0\n";
print EXPECTED "LENG 1094\n";
print EXPECTED "ALPH Amino\n";
print EXPECTED "//\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $hmmfile $expected_hmmfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_make_hmmfile: failed test3 (files $hmmfile $expected_hmmfile)\n"; exit;}
system "rm -f $input_hmms";
system "rm -f $hmmfile";
system "rm -f $expected_hmmfile";
# WRITE THE INPUT FILE OF HMMS:
$random_number = rand();
$input_hmms = $outputdir."/tmp".$random_number;
open(INPUT_HMMS,">$input_hmms") || die "ERROR: test_make_hmmfile: cannot open $input_hmms\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF350597.0\n";
print INPUT_HMMS "LENG 1094\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101000.0\n";
print INPUT_HMMS "LENG 1095\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
print INPUT_HMMS "HMMER2.0 [2.3.2]\n";
print INPUT_HMMS "NAME TF101001.0\n";
print INPUT_HMMS "LENG 1096\n";
print INPUT_HMMS "ALPH Amino\n";
print INPUT_HMMS "//\n";
close(INPUT_HMMS);
($hmmfile,$errorcode,$errormsg) = &make_hmmfile("TF350598",$outputdir,$input_hmms);
if ($errorcode != 9) { print STDERR "ERROR: test_make_hmmfile: failed test4\n"; exit;}
system "rm -f $input_hmms";
system "rm -f $hmmfile";
}
#------------------------------------------------------------------#
# MAKE A HMM FILE FOR A FAMILY:
sub make_hmmfile
{
my $family = $_[0]; # NAME OF TREEFAM FAMILY
my $outputdir = $_[1]; # DIRECTORY FOR WRITING OUTPUT FILES
my $input_hmms = $_[2]; # INPUT FILE OF HMMS FOR ALL FAMILIES
my $hmmfile; # FILE WITH HMM FOR THE FAMILY
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $line; #
my $take = 0; # SAYS WHETHER TO TAKE A PARTICULAR HMM FROM $input_hmms
my $prev_line; # PREVIOUS LINE READ IN FROM $input_hmms
my $found = 0; # SAYS WHETHER WE FOUND THE HMM FOR FAMILY $family IN $input_hmms
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my @temp; #
my $hmmname; # NAME OF THE HMM
# OPEN AN OUTPUT FILE FOR THE HMM FROM FAMILY $family:
$random_number = rand();
$hmmfile = $outputdir."/tmp".$random_number;
open(HMMFILE,">$hmmfile") || die "ERROR: make_hmmfile: cannot open $hmmfile\n";
# READ IN ONE HMM AT A TIME FROM THE INPUT FILE OF HMMs:
open(HMMS,"$input_hmms") || die "ERROR: make_hmmfile: cannot open $input_hmms\n";
while(<HMMS>)
{
$line = $_;
chomp $line;
if (substr($line,0,4) eq 'NAME')
{
@temp = split(/\s+/,$line);
$hmmname = $temp[1];
if ($hmmname =~ /\./)
{
@temp = split(/\./,$hmmname);
$hmmname = $temp[0];
}
if ($hmmname eq $family)
{
$take = 1;
$found = 1;
print HMMFILE "$prev_line\n";
}
}
if ($take == 1)
{
print HMMFILE "$line\n";
}
if (substr($line,0,2) eq '//')
{
if ($take == 1) { $take = 0;}
}
$prev_line = $line;
}
close(HMMS);
close(HMMFILE);
if ($found == 0)
{
$errormsg = "ERROR: make_hmms: did not find HMM for $family in $input_hmms\n";
$errorcode = 9; # ERRORCODE=9
return($hmmfile,$errorcode,$errormsg);
}
return($hmmfile,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &make_seqfile
sub test_make_seqfile
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES INTO
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
my $seqfile; # FASTA FILE WITH BITS OF A SCAFFOLD THAT HAVE BLAST HITS
my $START_COORD; # HASH TABLE TO STORE THE START COORDINATES OF REGIONS OF A SCAFFOLD THAT HAVE BLAST HITS
my $input_fasta; # FASTA FILE OF THE SCAFFOLDS
my %BLAST = (); # HASH TABLE OF THE BLAST HITS
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $expected_seqfile; # FILE CONTAINING THE EXPECTED CONTENTS OF $seqfile
my $differences; # DIFFERENCES BETWEEN $seqfile AND $expected_seqfile
my $length_differences; # LENGTH OF $differences
my $line; #
# OPEN AN INPUT FASTA FILE OF SCAFFOLD SEQUENCES:
$random_number = rand();
$input_fasta = $outputdir."/tmp".$random_number;
open(INPUT_FASTA,">$input_fasta") || die "ERROR: test_make_seqfile: cannot open $input_fasta\n";
print INPUT_FASTA ">scaffold1\n";
print INPUT_FASTA "ABCDEFGHIJ\n";
print INPUT_FASTA "KLMNOPQRST\n";
close(INPUT_FASTA);
$BLAST{"scaffold1"} = "-3=5,7=12,19=25";
$BLAST{"scaffold2"} = "-30=500,600=700";
($seqfile,$START_COORD,$errorcode,$errormsg) = &make_seqfile($input_fasta,$outputdir,\%BLAST,"scaffold1");
if ($errorcode != 0) { print STDERR "ERROR: test_make_seqfile: failed test1\n"; exit;}
# CHECK THE VALUES STORED IN $START_COORD ARE OK:
if ($START_COORD->{"HIT1"} != 1 || $START_COORD->{"HIT2"} != 7 || $START_COORD->{"HIT3"} != 19)
{
print STDERR "ERROR: test_make_seqfile: failed test1\n";
exit;
}
# OPEN A FILE CONTAINING THE EXPECTED CONTENTS OF $seqfile:
$random_number = rand();
$expected_seqfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_seqfile") || die "ERROR: test_make_seqfile: cannot open $expected_seqfile\n";
print EXPECTED ">HIT1\n";
print EXPECTED "ABCDE\n";
print EXPECTED ">HIT2\n";
print EXPECTED "GHIJKL\n";
print EXPECTED ">HIT3\n";
print EXPECTED "ST\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $seqfile $expected_seqfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_make_seqfile: failed test1 (files $seqfile $expected_seqfile)\n"; exit;}
system "rm -f $input_fasta";
system "rm -f $seqfile";
system "rm -f $expected_seqfile";
# OPEN AN INPUT FASTA FILE OF SCAFFOLD SEQUENCES:
$random_number = rand();
$input_fasta = $outputdir."/tmp".$random_number;
open(INPUT_FASTA,">$input_fasta") || die "ERROR: test_make_seqfile: cannot open $input_fasta\n";
print INPUT_FASTA ">scaffold1\n";
print INPUT_FASTA "ABCDEFGHIJ\n";
print INPUT_FASTA "KLMNOPQRST\n";
close(INPUT_FASTA);
$BLAST{"scaffold1"} = "-3=5,7=12,19=25";
$BLAST{"scaffold2"} = "-30=500,600=700";
($seqfile,$START_COORD,$errorcode,$errormsg) = &make_seqfile($input_fasta,$outputdir,\%BLAST,"scaffold2");
if ($errorcode != 16) { print STDERR "ERROR: test_make_seqfile: failed test2\n"; exit;}
system "rm -f $input_fasta";
}
#------------------------------------------------------------------#
# MAKE A FILE WITH THE BITS OF THE SCAFFOLD THAT HAVE HITS:
sub make_seqfile
{
my $input_fasta = $_[0]; # INPUT FASTA FILE OF SCAFFOLDS
my $outputdir = $_[1]; # DIRECTORY FOR WRITING OUTPUT FILES TO
my $BLAST = $_[2]; # HASH TABLE OF BLAST HITS
my $scaffold = $_[3]; # SCAFFOLD OF INTEREST
my $seqfile; # FASTA FILE WITH BITS OF THE SCAFFOLD THAT HAVE HITS
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $seq = ""; # SEQUENCE FOR THE SCAFFOLD
my $line; #
my $name; # NAME OF A SCAFFOLD IN $input_fasta
my @temp; #
my $take = 0; # SAYS WHETHER TO TAKE A PARTICULAR SEQUENCE
my $found = 0; # SAYS WHETHER WE FOUND THE SCAFFOLD SEQUENCE IN $input_fasta
my $hits; # REGIONS OF BLAST HITS IN THE SCAFFOLD
my @hits; # REGIONS OF BLAST HITS IN THE SCAFFOLD
my $hit; # A BLAST HIT IN THE SCAFFOLD
my $no_hits; # NUMBER OF BLAST HITS IN THE SCAFFOLD
my $i; #
my $hit_start; # START OF BLAST HIT IN THE SCAFFOLD
my $hit_end; # END OF BLAST HIT IN THE SCAFFOLD
my $seq_length; # LENGTH OF THE SEQUENCE FOR THE SCAFFOLD
my $hit_seq; # SEQUENCE IN THE REGION OF A HIT
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAME
my %START_COORD = (); # HASH TABLE TO STORE THE START COORDINATES OF $hit_seq IN THE SCAFFOLD
# GET THE SEQUENCE FOR THE SCAFFOLD:
open(INPUT_FASTA,"$input_fasta") || die "ERROR: make_seqfile: cannot open $input_fasta\n";
while(<INPUT_FASTA>)
{
$line = $_;
chomp $line;
@temp = split(/\s+/,$line);
if (substr($line,0,1) eq '>')
{
$name = $temp[0];
$name = substr($name,1,length($name)-1);
if ($name eq $scaffold) { $take = 1; $found = 1;}
else { $take = 0;}
}
elsif ($take == 1)
{
$seq = $seq.$line;
}
}
close(INPUT_FASTA);
if ($found == 0 || $seq eq '')
{
$errormsg = "ERROR: make_seqfile: did not find $scaffold in $input_fasta\n";
$errorcode = 16; # ERRORCODE=16
return($seqfile,\%START_COORD,$errorcode,$errormsg);
}
$seq_length = length($seq);
# MAKE A SEQUENCE FILE WITH THE BITS OF THE SCAFFOLD THAT HAVE BLAST MATCHES TO THE FAMILY:
$random_number = rand();
$seqfile = $outputdir."/tmp".$random_number;
open(SEQFILE,">$seqfile") || die "ERROR: make_seqfile: cannot open $seqfile\n";
$hits = $BLAST->{$scaffold};
@hits = split(/\,/,$hits);
$no_hits = $#hits + 1;
for ($i = 1; $i <= $no_hits; $i++)
{
$hit = $hits[($i-1)];
@temp = split(/=/,$hit);
$hit_start = $temp[0];
$hit_end = $temp[1];
if ($hit_start < 1) { $hit_start = 1;}
if ($hit_end > $seq_length) { $hit_end = $seq_length;}
# TAKE THE SEQUENCE IN THE SCAFFOLD FROM $hit_start TO $hit_end:
$hit_seq = substr($seq,$hit_start-1,$hit_end-$hit_start+1);
print SEQFILE ">HIT$i\n";
print SEQFILE "$hit_seq\n";
# RECORD THE START COORDINATE OF $hit_seq IN THE SCAFFOLD
if ($START_COORD{"HIT".$i})
{
$errormsg = "ERROR: make_seqfile: already have start coordinate for HIT$i\n";
$errorcode = 18; # ERRORCODE=18
return($seqfile,\%START_COORD,$errorcode,$errormsg);
}
$START_COORD{"HIT".$i} = $hit_start;
}
close(SEQFILE);
return($seqfile,\%START_COORD,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &convert_to_scaffold_coordinates
sub test_convert_to_scaffold_coordinates
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES INTO
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
my $genewise_output; # GENEWISE OUTPUT FILE
my %START_COORD = (); # HASH TABLE WITH START COORDINATES OF CHUNKS OF DNA IN SCAFFOLDS
my $genewise_output2; # GENEWISE OUTPUT FILE, CONVERTED TO SCAFFOLD COORDINATES
my $expected_genewise_output2; # FILE WITH EXPECTED CONTENTS OF $genewise_output2
my $differences; # DIFFERENCES BETWEEN $genewise_output2 AND $expected_genewise_output2
my $length_differences; # LENGTH OF $differences
my $line; #
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
# OPEN A FILE WITH GENEWISE OUTPUT:
$random_number = rand();
$genewise_output = $outputdir."/tmp".$random_number;
open(GENEWISE_OUTPUT,">$genewise_output") || die "ERROR: test_convert_to_scaffold_coordinates: cannot open $genewise_output\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 2 4 -19.50 + . HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 2 4 0.00 + 0 HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 7 24 0.91 + . HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 7 24 0.00 + 0 HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 2 4 -19.50 + . HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 2 4 0.00 + 0 HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 7 24 0.91 + . HIT2-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 7 24 0.00 + 0 HIT2-genewise-prediction-2\n";
close(GENEWISE_OUTPUT);
$START_COORD{"HIT1"} = 100;
$START_COORD{"HIT2"} = 200;
($genewise_output2,$errorcode,$errormsg) = &convert_to_scaffold_coordinates($genewise_output,$outputdir,"scaffold1",\%START_COORD,'TF101000');
if ($errorcode != 0) { print STDERR "ERROR: test_convert_to_scaffold_coordinates: failed test1\n"; exit;}
# OPEN A FILE WITH THE EXPECTED OUTPUT:
$random_number = rand();
$expected_genewise_output2 = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_genewise_output2") || die "ERROR: test_convert_to_scaffold_coordinates: cannot open $expected_genewise_output2";
print EXPECTED "scaffold1 GeneWise match 101 103 -19.50 + . scaffold1-TF101000-genewise-prediction-1\n";
print EXPECTED "scaffold1 GeneWise cds 101 103 0.00 + 0 scaffold1-TF101000-genewise-prediction-1\n";
print EXPECTED "scaffold1 GeneWise match 106 123 0.91 + . scaffold1-TF101000-genewise-prediction-2\n";
print EXPECTED "scaffold1 GeneWise cds 106 123 0.00 + 0 scaffold1-TF101000-genewise-prediction-2\n";
print EXPECTED "scaffold1 GeneWise match 201 203 -19.50 + . scaffold1-TF101000-genewise-prediction-3\n";
print EXPECTED "scaffold1 GeneWise cds 201 203 0.00 + 0 scaffold1-TF101000-genewise-prediction-3\n";
print EXPECTED "scaffold1 GeneWise match 206 223 0.91 + . scaffold1-TF101000-genewise-prediction-4\n";
print EXPECTED "scaffold1 GeneWise cds 206 223 0.00 + 0 scaffold1-TF101000-genewise-prediction-4\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $genewise_output2 $expected_genewise_output2 |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_convert_to_scaffold_coordinates: failed test1 (files $genewise_output2 $expected_genewise_output2)\n"; exit;}
system "rm -f $genewise_output";
system "rm -f $genewise_output2";
system "rm -f $expected_genewise_output2";
# OPEN A FILE WITH GENEWISE OUTPUT:
$random_number = rand();
$genewise_output = $outputdir."/tmp".$random_number;
open(GENEWISE_OUTPUT,">$genewise_output") || die "ERROR: test_convert_to_scaffold_coordinates: cannot open $genewise_output\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 2 4 -19.50 + . HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 2 4 0.00 + 0 HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 7 24 0.91 + . HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 7 24 0.00 + 0 HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 2 4 -19.50 + . HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 2 4 0.00 + 0 HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 7 24 0.91 + . HIT2-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 7 24 0.00 + 0 HIT2-genewise-prediction-2\n";
close(GENEWISE_OUTPUT);
%START_COORD = ();
($genewise_output2,$errorcode,$errormsg) = &convert_to_scaffold_coordinates($genewise_output,$outputdir,"scaffold1",\%START_COORD,'TF101000');
if ($errorcode != 19) { print STDERR "ERROR: test_convert_to_scaffold_coordinates: failed test2\n"; exit;}
system "rm -f $genewise_output";
system "rm -f $genewise_output2";
# OPEN A FILE WITH GENEWISE OUTPUT:
$random_number = rand();
$genewise_output = $outputdir."/tmp".$random_number;
open(GENEWISE_OUTPUT,">$genewise_output") || die "ERROR: test_convert_to_scaffold_coordinates: cannot open $genewise_output\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 2 4 -19.50 + . HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 2 4 0.00 + 0 HIT1-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT1 GeneWise match 7 24 0.91 + . HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT1 GeneWise cds 7 24 0.00 + 0 HIT1-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 2 4 -19.50 + . HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 2 4 0.00 + 0 HIT2-genewise-prediction-1\n";
print GENEWISE_OUTPUT "HIT2 GeneWise match 7 24 0.91 + . HIT2-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise cds 7 24 0.00 + 0 HIT2-genewise-prediction-2\n";
print GENEWISE_OUTPUT "HIT2 GeneWise\n";
close(GENEWISE_OUTPUT);
$START_COORD{"HIT1"} = 100;
$START_COORD{"HIT2"} = 200;
($genewise_output2,$errorcode,$errormsg) = &convert_to_scaffold_coordinates($genewise_output,$outputdir,"scaffold1",\%START_COORD,'TF101000');
if ($errorcode != 17) { print STDERR "ERROR: test_convert_to_scaffold_coordinates: failed test3\n"; exit;}
system "rm -f $genewise_output";
system "rm -f $genewise_output2";
}
#------------------------------------------------------------------#
# CHANGE THE GENEWISE COORDINATES BACK TO SCAFFOLD COORDINATES:
sub convert_to_scaffold_coordinates
{
my $genewise_output = $_[0]; # GENEWISE OUTPUT FOR ONE SCAFFOLD AND ONE HMM
my $outputdir = $_[1]; # DIRECTORY TO WRITE OUTPUT FILES INTO
my $scaffold = $_[2]; # NAME OF THE SCAFFOLD
my $START_COORD = $_[3]; # START COORDINATE OF SEQUENCE USED FOR RUNNING GENEWISE
my $family = $_[4]; # NAME OF TREEFAM FAMILY
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $genewise_output2 = "none";# FILE WITH GENEWISE OUTPUT IN SCAFFOLD COORDAINTES
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $line; #
my @temp; #
my $start; # START OF FEATURE IN GENEWISE OUTPUT FILE
my $end; # END OF FEATURE IN GENEWISE OUTPUT FILE
my $name; # NAME OF FEATURE IN GENEWISE OUTPUT FILE
my $seqname; # NAME OF SEQUENCE USED FOR RUNNING GENEWISE
my $start_coord; # START COORDINATE OF $seqname IN THE SCAFFOLD
my $new_start; # START OF THE FEATURE, WITH RESPECT TO THE SCAFFOLD
my $new_end; # END OF THE FEATURE, WITH RESPECT TO THE SCAFFOLD
my $new_name; # NEW NAME OF THE FEATURE, USING THE SCAFFOLD NAME
my $num_predictions = 0; # NUMBER OF GENE PREDICTIONS SEEN IN A SCAFFOLD
my %SEEN = (); # HASH TABLE TO RECORD WHETHER WE HAVE SEEN A PARTICULAR GENE PREDICTION BEFORE
# MAKE A TEMPORARY FILE TO PUT THE GENEWISE OUTPUT WITH SCAFFOLD COORDINATES INTO:
$random_number = rand();
$genewise_output2 = $outputdir."/tmp".$random_number;
open(GENEWISE_OUTPUT2,">$genewise_output2") || die "ERROR: convert_to_scaffold_coordinates: cannot open $genewise_output2\n";
# READ IN THE INPUT FILE OF GENEWISE OUTPUT:
open(GENEWISE_OUTPUT,"$genewise_output") || die "ERROR: convert_to_scaffold_coordinates: cannot open $genewise_output";
while(<GENEWISE_OUTPUT>)
{
$line = $_;
chomp $line;
@temp = split(/\t+/,$line);
if ($#temp == 8)
{
$seqname = $temp[0];
$start = $temp[3];
$end = $temp[4];
$name = $temp[8];
# FIND THE START COORDINATE OF $seqname IN THE SCAFFOLD:
if (!($START_COORD->{$seqname}))
{
$errormsg = "ERROR: convert_to_scaffold_coordinates: do not know start coordinate of $seqname\n";
$errorcode = 19; # ERRORCODE=19
return($genewise_output2,$errorcode,$errormsg);
}
$start_coord = $START_COORD->{$seqname};
# WORK OUT THE NEW START, END AND NAME:
$new_start = $start + $start_coord - 1;
$new_end = $end + $start_coord - 1;
if (!($SEEN{$name}))
{
$SEEN{$name} = 1;
$num_predictions++;
}
$new_name = $scaffold."-".$family."-genewise-prediction-".$num_predictions;
# PRINT OUT TO $genewise_output2:
print GENEWISE_OUTPUT2 "$scaffold\t$temp[1]\t$temp[2]\t$new_start\t$new_end\t$temp[5]\t$temp[6]\t$temp[7]\t$new_name\n";
}
elsif ($#temp > 0)
{
$errormsg = "ERROR: convert_to_scaffold_coordinates: line $line in $genewise_output\n";
$errorcode = 17; # ERRORCODE=17
return($genewise_output2,$errorcode,$errormsg);
}
}
close(GENEWISE_OUTPUT);
close(GENEWISE_OUTPUT2);
return($genewise_output2,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# RUN GENEWISE WITH ONE SCAFFOLD/CONTIG AT A TIME, ON ONE HMM AT A TIME:
sub run_genewise_on_scaffolds2
{
my $outputdir = $_[0]; # THE DIRECTORY FOR WRITING OUTPUT FILES
my $input_fasta = $_[1]; # THE INPUT FASTA FILE OF SCAFFOLDS
my $input_hmms = $_[2]; # THE INPUT FILE OF HMMs
my $output = $_[3]; # THE OUTPUT FILE
my $spliceflat = $_[4]; # SAYS WHETHER TO USE THE SPLICE FLAT OPTION
my $parameterfile = $_[5]; # THE INTRON PARAMETER FILE NAME
my $treefam_seqs = $_[6]; # FILE WITH PROTEIN SEQUENCES IN TREEFAM FAMILIES
my $evalue_cutoff = $_[7]; # EVALUE CUTOFF TO USE FOR BLAST MATCHES
my $flank_length = $_[8]; # LENGTH OF SEQUENCE TO TAKE ON EITHER SIDE OF A BLAST MATCH
my $testing = $_[9]; # SAYS WHETHER THIS SUBROUTINE IS BEING CALLED FROM A TESTING FUNCTION
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $line; #
my @temp; #
my $family = "none";# TREEFAM FAMILY NAME
my $seqfile; # FILE CONTAINING THE SEQUENCES FOR A FAMILY
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAME
my $BLAST; # HASH TABLE WITH POSITIONS OF BLAST MATCHES IN SCAFFOLDS
my $hit; # REGION OF A BLAST HIT BETWEEN THE FAMILY AND A SCAFFOLD
my $scaffold; # A SCAFFOLD SEQUENCE
my $hits; # REGIONS OF BLAST HITS BETWEEN THE FAMILY AND A SCAFFOLD
my @hits; # REGIONS OF BLAST HITS BETWEEN THE FAMILY AND A SCAFFOLD
my $no_hits; # NUMBER OF BLAST HITS BETWEEN THE FAMILY AND A SCAFFOLD
my $hitstart; # BLAST HIT START IN THE SCAFFOLD
my $hitend; # BLAST HIT END IN THE SCAFFOLD
my $i; #
my $no_scaffolds_with_hits; # NUMBER OF SCAFFOLDS WITH HITS
my $hmmfile; # FILE CONTAINING THE HMM
my $genewise_output; # FILE CONTAINING GENEWISE OUTPUT FOR ONE SCAFFOLD AND ONE HMM
my $genewise_output2; # FILE CONTAINING GENEWISE OUTPUT FOR ONE SCAFFOLD AND ONE HMM, IN SCAFFOLD COORDINATES
my $START_COORD; # HASH TABLE WITH START COORDINATES IN SCAFFOLDS, OF SEQUENCES TAKEN FOR RUNNING GENEWISE
my $seqfile2; # FILE CONTAINING DNA SEQUENCE OF REGIONS AROUND BLAST HITS
my $formatdbfile; # FORMATDB FORMATTING FILE
# OPEN THE OUTPUT FILE FOR THE GENEWISE OUTPUT:
$output = $outputdir."/".$output;
open(OUTPUT,">$output") || die "ERROR: run_genewise_on_scaffolds2: cannot open $output\n";
close(OUTPUT);
# RUN FORMATDB ON THE SCAFFOLDS:
system "formatdb -i $input_fasta -p F -o T";
# READ IN THE SEQUENCES IN EACH FAMILY, FAMILY BY FAMILY:
open(TREEFAM_SEQS,"$treefam_seqs") || die "ERROR: get_seqs_in_family: cannot open $treefam_seqs\n";
while(<TREEFAM_SEQS>)
{
$line = $_;
chomp($line);
@temp = split(/\s+/,$line);
if ($#temp == 0 && (substr($line,0,3) eq "TF1" || substr($line,0,3) eq "TF3") && length($line) == 8)
{
$family = $temp[0];
# OPEN A TEMPORARY FILE FOR THE SEQUENCES FOR THIS FAMILY:
$random_number = rand();
$seqfile = $outputdir."/tmp".$random_number;
open(SEQFILE,">$seqfile") || die "ERROR: run_genewise_on_scaffolds2: cannot open $seqfile\n";
# WRITE THE FAMILY NAME IN THE OUTPUT FILE:
system "echo $family >> $output";
}
elsif ($line eq "#END")
{
# CLOSE THE FILE WITH SEQUENCES FOR THIS FAMILY:
close(SEQFILE);
# RUN BLAST BETWEEN THE FILE WITH SEQUENCES FOR THIS FAMILY AND THE FILE OF SCAFFOLDS:
if ($testing == 0) { print STDERR "Checking for blast matches for family $family in the scaffolds...\n"; }
($BLAST,$errorcode,$errormsg) = &check_for_blast_match2($seqfile,$input_fasta,$outputdir,$evalue_cutoff,$flank_length);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
# CHECK IF THERE ARE ANY SCAFFOLDS WITH HITS:
$no_scaffolds_with_hits = keys %{$BLAST};
if ($no_scaffolds_with_hits >= 1)
{
# MAKE A FILE WITH THE HMM FOR THE FAMILY:
($hmmfile,$errorcode,$errormsg) = &make_hmmfile($family,$outputdir,$input_hmms);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
# AS THERE ARE BLAST MATCHES BETWEEN THIS FAMILY AND THE SCAFFOLDS, RUN GENEWISE
# FOR THE FAMILY AGAINST THE REGIONS OF THOSE SCAFFOLDS:
foreach $scaffold (keys %{$BLAST})
{
# MAKE A FILE WITH THE BITS OF THE SCAFFOLDS THAT HAVE HITS:
($seqfile2,$START_COORD,$errorcode,$errormsg) = &make_seqfile($input_fasta,$outputdir,$BLAST,$scaffold);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
# RUN GENEWISE FOR THE HMM AGAINST THE SCAFFOLD SEQUENCES:
($genewise_output,$errorcode,$errormsg) = &run_genewise_on_hmm($hmmfile,$outputdir,$seqfile2,$family,$spliceflat,$parameterfile,$scaffold,$testing);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
# CHANGE THE GENEWISE COORDINATES BACK TO SCAFFOLD COORDINATES:
($genewise_output2,$errorcode,$errormsg) = &convert_to_scaffold_coordinates($genewise_output,$outputdir,$scaffold,$START_COORD,$family);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
# CONCATENATE THIS GENEWISE OUTPUT ONTO THE MAIN OUTPUT FILE OF GENEWISE OUTPUT:
system "cat $genewise_output2 >> $output";
sleep(1);
# DELETE TEMPORARY FILES:
system "rm -f $seqfile2";
system "rm -f $genewise_output";
system "rm -f $genewise_output2";
}
# DELETE TEMPORARY HMMFILE:
system "rm -f $hmmfile";
}
# DELETE THE TEMPORARY FILE WITH SEQUENCES FOR THIS FAMILY:
system "rm -f $seqfile";
system "echo '//' >> $output";
}
else
{
print SEQFILE "$line\n";
}
}
close(TREEFAM_SEQS);
# DELETE THE FORMATDB OUTPUT FILES:
$formatdbfile = $input_fasta.".nhr";
system "rm -f $formatdbfile";
$formatdbfile = $input_fasta.".nin";
system "rm -f $formatdbfile";
$formatdbfile = $input_fasta.".nsd";
system "rm -f $formatdbfile";
$formatdbfile = $input_fasta.".nsi";
system "rm -f $formatdbfile";
$formatdbfile = $input_fasta.".nsq";
system "rm -f $formatdbfile";
return($errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &write_fasta_file
sub test_write_fasta_file
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES TO
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
my $fasta; # FASTA FILE
my $expected_fasta; # EXPECTED CONTENTS OF FASTA FILE
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $differences; # DIFFERENCES BETWEEN $fasta AND $expected_fasta
my $length_differences; # LENGTH OF $differences
my $line; #
($fasta,$errorcode,$errormsg) = &write_fasta_file("AGCTAGCT","myseq",$outputdir);
if ($errorcode != 0) { print STDERR "ERROR: test_write_fasta_file: failed test1\n"; exit;}
$random_number = rand();
$expected_fasta = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_fasta") || die "ERROR: write_fasta_file: cannot open $expected_fasta\n";
print EXPECTED ">myseq\n";
print EXPECTED "AGCTAGCT\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $fasta $expected_fasta |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_write_fasta_file: failed test1 (files $fasta $expected_fasta)\n"; exit;}
system "rm -f $fasta";
system "rm -f $expected_fasta";
}
#------------------------------------------------------------------#
# WRITE THE SEQUENCE TO A TEMPORARY FASTA FILE:
sub write_fasta_file
{
my $seq = $_[0]; # SEQUENCE
my $name = $_[1]; # NAME OF THE SEQUENCE
my $outputdir = $_[2]; # DIRECTORY FOR WRITING OUTPUT FILES TO
my $fasta; # FASTA FILE
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
$random_number = rand();
$fasta = $outputdir."/tmp".$random_number;
open(FASTA,">$fasta") || die "ERROR: write_fasta_file: cannot open $fasta\n";
print FASTA ">$name\n";
print FASTA "$seq\n";
close(FASTA);
return($fasta,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# CHECK THAT THE WISECONFIGDIR IS SET CORRECTLY:
sub check_wiseconfigdir
{
my $wiseconfigdir = "none";# THE WISECONFIGDIR
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
open(TMP,"echo \$WISECONFIGDIR |");
while(<TMP>)
{
$wiseconfigdir = $_;
chomp($wiseconfigdir);
}
close(TMP);
if ($wiseconfigdir ne '/nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/wisecfg/')
{
$errormsg = "ERROR: check_wiseconfigdir: wiseconfigdir $wiseconfigdir\nBefore running this perl script, you must run:\nsetenv WISECONFIGDIR /nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/wisecfg/\n";
$errorcode = 6; # ERRORCODE=6
return($errorcode,$errormsg);
}
return($errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &get_seqs_in_family
sub test_get_seqs_in_family
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES INTO
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAMES
my $seqfile; # FILE WITH SEQUENCES FOR A FAMILY
my $treefam_seqs; # FILE WITH SEQUENCES IN TREEFAM FAMILIES
my $errorcode; # RETURNED AS 0 FROM A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' FROM A FUNCTION IF THERE IS NO ERROR
my $expected_seqfile; # FILE WITH EXPECTED OUTPUT
my $differences; # DIFFERENCES BETWEEN $seqfile AND $expected_seqfile
my $length_differences; # LENGTH OF $differences
my $line; #
$random_number = rand();
$treefam_seqs = $outputdir."/tmp".$random_number;
open(FILE,">$treefam_seqs") || die "ERROR: test_get_seqs_in_family: cannot open $treefam_seqs\n";
print FILE "TF101001\n";
print FILE ">seq1\n";
print FILE "AGCGTAG\n";
print FILE "AGTATAT\n";
print FILE ">seq2\n";
print FILE "CAGAGTA\n";
print FILE "CCCAGAT\n";
print FILE "ATTACAG\n";
print FILE ">seq3\n";
print FILE "ATATATA\n";
print FILE "#END\n";
print FILE "TF101002\n";
print FILE ">seq4\n";
print FILE "AATCAGAG\n";
print FILE ">seq5\n";
print FILE "AATCAGTA\n";
print FILE "#END\n";
close(FILE);
($seqfile,$errorcode,$errormsg) = &get_seqs_in_family($treefam_seqs,$outputdir,"TF101001");
if ($errorcode != 0) { print STDERR "ERROR: test_get_seqs_in_family: failed test1 (errorcode $errorcode errormsg $errormsg)\n"; exit;}
$random_number = rand();
$expected_seqfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_seqfile") || die "ERROR: test_get_seqs_in_family: cannot open $expected_seqfile\n";
print EXPECTED ">seq1\n";
print EXPECTED "AGCGTAG\n";
print EXPECTED "AGTATAT\n";
print EXPECTED ">seq2\n";
print EXPECTED "CAGAGTA\n";
print EXPECTED "CCCAGAT\n";
print EXPECTED "ATTACAG\n";
print EXPECTED ">seq3\n";
print EXPECTED "ATATATA\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $seqfile $expected_seqfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_get_seqs_in_family: failed test1 (files $seqfile $expected_seqfile)\n"; exit;}
system "rm -f $seqfile";
system "rm -f $expected_seqfile";
system "rm -f $treefam_seqs";
$random_number = rand();
$treefam_seqs = $outputdir."/tmp".$random_number;
open(FILE,">$treefam_seqs") || die "ERROR: test_get_seqs_in_family: cannot open $treefam_seqs\n";
print FILE "TF101001\n";
print FILE ">seq1\n";
print FILE "AGCGTAG\n";
print FILE "AGTATAT\n";
print FILE ">seq2\n";
print FILE "CAGAGTA\n";
print FILE "CCCAGAT\n";
print FILE "ATTACAG\n";
print FILE ">seq3\n";
print FILE "ATATATA\n";
print FILE "#END\n";
print FILE "TF101002\n";
print FILE ">seq4\n";
print FILE "AATCAGAG\n";
print FILE ">seq5\n";
print FILE "AATCAGTA\n";
print FILE "#END\n";
close(FILE);
($seqfile,$errorcode,$errormsg) = &get_seqs_in_family($treefam_seqs,$outputdir,"TF101002");
if ($errorcode != 0) { print STDERR "ERROR: test_get_seqs_in_family: failed test2\n"; exit;}
$random_number = rand();
$expected_seqfile = $outputdir."/tmp".$random_number;
open(EXPECTED,">$expected_seqfile") || die "ERROR: test_get_seqs_in_family: cannot open $expected_seqfile\n";
print EXPECTED ">seq4\n";
print EXPECTED "AATCAGAG\n";
print EXPECTED ">seq5\n";
print EXPECTED "AATCAGTA\n";
close(EXPECTED);
$differences = "";
open(TEMP,"diff $seqfile $expected_seqfile |");
while(<TEMP>)
{
$line = $_;
$differences = $differences.$line;
}
close(TEMP);
$length_differences = length($differences);
if ($length_differences != 0) { print STDERR "ERROR: test_get_seqs_in_family: failed test2 (files $seqfile $expected_seqfile)\n"; exit;}
system "rm -f $seqfile";
system "rm -f $expected_seqfile";
system "rm -f $treefam_seqs";
$random_number = rand();
$treefam_seqs = $outputdir."/tmp".$random_number;
open(FILE,">$treefam_seqs") || die "ERROR: test_get_seqs_in_family: cannot open $treefam_seqs\n";
print FILE "TF101001\n";
print FILE ">seq1\n";
print FILE "AGCGTAG\n";
print FILE "AGTATAT\n";
print FILE ">seq2\n";
print FILE "CAGAGTA\n";
print FILE "CCCAGAT\n";
print FILE "ATTACAG\n";
print FILE ">seq3\n";
print FILE "ATATATA\n";
print FILE "#END\n";
print FILE "TF101002\n";
print FILE ">seq4\n";
print FILE "AATCAGAG\n";
print FILE ">seq5\n";
print FILE "AATCAGTA\n";
print FILE "#END\n";
close(FILE);
($seqfile,$errorcode,$errormsg) = &get_seqs_in_family($treefam_seqs,$outputdir,"TF101003");
if ($errorcode != 7) { print STDERR "ERROR: test_get_seqs_in_family: failed test3\n"; exit;}
system "rm -f $seqfile";
system "rm -f $treefam_seqs";
}
#------------------------------------------------------------------#
# PUT THE SEQUENCES FOR A FAMILY IN A FASTA FILE:
sub get_seqs_in_family
{
my $treefam_seqs = $_[0]; # FILE WITH SEQUENCES IN TREEFAM FAMILIES
my $outputdir = $_[1]; # DIRECTORY FOR WRITING OUTPUT FILES TO
my $family = $_[2]; # NAME OF THE TREEFAM FAMILY
my $found = 0; # SAYS WHEN WE FOUND A FAMILY IN $treefam_seqs
my $seqfile; # FILE WITH SEQUENCES FOR A FAMILY
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAME
my $line; #
my $found_fam = 0; # SAYS WHETHER WE FOUND A FAMILY IN $treefam_seqs
# OPEN AN OUTPUT FILE FOR THE SEQUENCES IN THE FAMILY:
$random_number = rand();
$seqfile = $outputdir."/tmp".$random_number;
open(SEQFILE,">$seqfile") || die "ERROR: get_seqs_in_family: cannot open $seqfile\n";
# READ IN THE SEQUENCES IN TREEFAM FAMILIES:
open(TREEFAM_SEQS,"$treefam_seqs") || die "ERROR: get_seqs_in_family: cannot open $treefam_seqs\n";
while(<TREEFAM_SEQS>)
{
$line = $_;
chomp $line;
if ($line eq $family) { $found = 1; $found_fam = 1;}
elsif ($line eq '#END'){ $found = 0;}
elsif ($found == 1) { print SEQFILE "$line\n";}
}
close(TREEFAM_SEQS);
close(SEQFILE);
if ($found_fam == 0)
{
$errormsg = "ERROR: get_seqs_in_family: did not find $family in $treefam_seqs\n";
$errorcode = 7; # ERRORCODE=7
return($seqfile,$errorcode,$errormsg);
}
return($seqfile,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &check_for_blast_match2
sub test_check_for_blast_match2
{
my $outputdir = $_[0]; # DIRECTORY TO WRITE OUTPUT FILES TO
my $errorcode; # RETURNED AS 0 FROM A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' FROM A FUNCTION IF THERE IS NO ERROR
my $seqfile; # FILE WITH THE PROTEIN SEQUENCES
my $scaffolds; # FILE WITH THE DNA SEQUENCES OF THE SCAFFOLDS
my $BLAST; # HASH TABLE WITH POSITIONS OF HITS IN SCAFFOLDS
my $random_number; # RANDOM NUMBER TO USE IN TEMPORARY FILE NAME
my $formatdbfile; # FORMAT FILE PRODUCED BY formatdb
# MAKE A FILE WITH THE PROTEIN SEQUENCES:
$random_number = rand();
$seqfile = $outputdir."/tmp".$random_number;
open(SEQFILE,">$seqfile") || die "ERROR: test_check_for_blast_match2: cannot open $seqfile\n";
print SEQFILE ">FOX-1\n";
print SEQFILE "MQALYQLSATGAQQQNQQIPIGLSNSLLYQQLAAHQQIAAQQHQQQLAVSAAHQTQNNIMLATSAPSLINHMENSTDGKV\n";
print SEQFILE "KDDPNSDYDLQLSIQQQLAAAAQAAQMGQTQIGPQIVGQQGQPVVATTAGSTNGSAAVTQPDPSTSSGPDGPKRLHVSNI\n";
print SEQFILE "PFRFRDPDLKTMFEKFGVVSDVEIIFNERGSKGFGFVTMERPQDAERARQELHGSMIEGRKIEVNCATARVHSKKVKPTG\n";
print SEQFILE "GILDQMNPLMAQSALAAQAQMNRALLLRSPLVAQSLLGRGAALIPGMQQPAFQLQAALAGNPLAQLQGQPLLFNAAALQT\n";
print SEQFILE "NALQQSAFGMDPAAVQAALLANEQARFQLAAAAAQGNEYIMYHQAKQQELPGRIPSSGNASAFGEQYLSNALATASLPSY\n";
print SEQFILE "QMNPALRTLNRFTPY\n";
close(SEQFILE);
# MAKE A FILE WITH THE DNA SEQUENCES:
$random_number = rand();
$scaffolds = $outputdir."/tmp".$random_number;
open(SCAFFOLDS,">$scaffolds") || die "ERROR: test_check_for_blast_match2: cannot open $scaffolds\n";
print SCAFFOLDS ">X:2445317..2450316\n";
print SCAFFOLDS "ttttcctgtaaagcaaagcattcttgctcattcaccacaatgccacattattgtgcttct\n";
print SCAFFOLDS "tcgtttcacagtctgcacaacaaaaacccaatcattcacgtgtttctgaaccttgttttg\n";
print SCAFFOLDS "ttttttgggaaccaagcagcttcacaccacatttaaggaaaagattcatgtacaccaagag\n";
print SCAFFOLDS "tacgtggaaaaaaaagaagaagaagtaagaaacacaaaggagtactggaaagcaaatgaac\n";
print SCAFFOLDS "tggtgggtgttgtgtgggaaaaagacaaggataaatagacatgaaaaaaaagatgcggaaa\n";
print SCAFFOLDS "taacgtttttttttttttgaaaatcaatccggatgcaaacacaaaaattggtggagatgcg\n";
print SCAFFOLDS "ttttaaattcaacatgaaagaaaaatgttgattaaaattatggaaacttacaattaaagct\n";
print SCAFFOLDS "tgaaaattattattgaaaaatgtgaaaaaatgtgtgatgggataaaggtgtaaaagaaata\n";
print SCAFFOLDS "taaaacttctcatattcaaacaaaaaattacgccgtattgttgataaaactccggagacta\n";
print SCAFFOLDS "tgcataaaacggggggaagaataggagttatcgcgttgctgtgaaatgtgatacaatcgaa\n";
print SCAFFOLDS "taatacatatgtatcgaatcataaaggggtacaactacaactacattgagaaaagtttttt\n";
print SCAFFOLDS "tcagagaggatttacaacaaagaaagttgtttctgagtcaaaaaaaagtgaggtcaggaat\n";
print SCAFFOLDS "ttgaatatcgtttaccaaaacggggggccactcaatacggagtaaatcgatttaacgttct\n";
print SCAFFOLDS "taagcgccgggttcatttgatatgaggggagtgaggcggtggccaacgcgttgctaaggta\n";
print SCAFFOLDS "tttgttcgccaaacgccgaggcatttccagatgatgggatccgtcctggtaattcttgttg\n";
print SCAFFOLDS "ttttggcttgatggtacataatgtactcgttaccttgagcagcggcagcagcgagttgaaa\n";
print SCAFFOLDS "tccgagcttgctcattggcaagcagagcagcttgaacggcggccggatccattccaaacgc\n";
print SCAFFOLDS "tcgactgttggagtgcgttcgtttgaagggctgcagcgttgaacagcaaaggttgaccttg\n";
print SCAFFOLDS "taagttgtgccagcggattgccagccaaagccgcttgcaattggaacgcgggctgttgcat\n";
print SCAFFOLDS "ttccggggattaaagcggctccgcgaccaagaagagattgtgctaccaatggactacggag\n";
print SCAFFOLDS "tcaatagggctctgttcatctgagcctgtgcggcaagagctgattgggccatcagtgggtt\n";
print SCAFFOLDS "tcatttggtccaggattcctaaataaataaattttgtttggcaatggttattgactagttt\n";
print SCAFFOLDS "tacctccagttggtttaactttcttcgagtgaacacgagctgtagcgcagttgacttcaat\n";
print SCAFFOLDS "ttttccgtccttcaatcattgatccatgaagctcttgtctagctctctcagcatcctgcgg\n";
print SCAFFOLDS "ttctctccattgtcacaaatccaaacccctgaaatataaatgtttatatgagctcagttcc\n";
print SCAFFOLDS "ttttctagtgttattgtactcacctttgatcctcgctcattgaaaataatttctacgtcgg\n";
print SCAFFOLDS "taaaccacaccaaatttctcaaacatcgttttgaggtctgggtctctgaatctgaatggga\n";
print SCAFFOLDS "ttattcgatacgtgaagtctttttggtccatcgggtccggctgaaaaaaattataatttgg\n";
print SCAFFOLDS "tttgaatttttgtgatgttagattccgtctaggccgactttatcatctttctttttttgaa\n";
print SCAFFOLDS "ttcattcagtggcaagcaaaaaaactctacaccgtttgcactaccttggttacgtgtgaat\n";
print SCAFFOLDS "ttaactttgatgacaagagaaaaataaagatgatacaaataatgccaatttgcgtgttaat\n";
print SCAFFOLDS "tcactgttttaatctttgtcacagttcagaaagaaaccgtttttccatgcaaattcaagac\n";
print SCAFFOLDS "tgttgaatataaaagatgtaaaagagaacagcacgaataaaactcaagaaagttgcgattt\n";
print SCAFFOLDS "ttgtatatatcgggaagagaaaaggagggcaaggcttacagctagtgctgggatcgggctg\n";
print SCAFFOLDS "ttgtgacggccgccgagccattcgtcgaaccggccgttgtggcgactaccggctgcccttg\n";
print SCAFFOLDS "tttgtcccacaatttgtggaccaatctgcgtttgtcccatctgagcagcttgcgctgccgc\n";
print SCAFFOLDS "tcgccaattgttgctgaatcgagagttgcaaatcgtaatcactgttcggatcgtcctgtaa\n";
print SCAFFOLDS "taacaaaaagcgctcattcacagatcatgcgccttaagaaaatggttttctttttttgggg\n";
print SCAFFOLDS "taatcgccgctctttcaattccagagcatgccaagttcacaaccgtgcacatctctgctct\n";
print SCAFFOLDS "tgagatgcaacacaccacctctcaccgtgtttagattaattagtttaaaaatgtttttgtg\n";
print SCAFFOLDS "tatgacgtgtaaaaaaaatgaaaatccgaaaatagaaagcagctaggggaaagttgaacac\n";
print SCAFFOLDS "tgtataatttttcgaagtgaaactatttaaaggaggacgattttgaacgttcagacccaaa\n";
print SCAFFOLDS "tatgggctgaaataaatgaattttgtaatgaaacttttcaaaaacttttcaaaaattcttt\n";
print SCAFFOLDS "tatggcggttcaaaatttcgaaaacaaaaaacactgattttagctaaaatctcaaattttg\n";
print SCAFFOLDS "tcttcttttctatgcggctgcggtacgaaattaactttttttgaattttcctcctttattg\n";
print SCAFFOLDS "tcatattttggtacatttttaaggtacatttaaagccgatagttaactaatttcaataatt\n";
print SCAFFOLDS "ttttggttacctatcggctttaaatgtacctcaacgaaattatatgagataaactacaaaa\n";
print SCAFFOLDS "taatgcaagaaaggatgataattcaaaaaaaaagtcatttcggacctctgccacacagaaa\n";
print SCAFFOLDS "tagtgggcaaacttcgagatcttagctgaaatcggtataatttttttcgaaattttaaacc\n";
print SCAFFOLDS "tgacaaaaaaaatttttgaagattttttgaaaattttttgaaaagtttcatttcaaatttt\n";
print SCAFFOLDS "tgttcttttgagcccattttgggtatataggttcaagttcgtccgaaccgttattccacct\n";
print SCAFFOLDS "tttaagcttaatatgaaatccggtagaaacatgtcatagtgttcagaaaaccgaaacacga\n";
print SCAFFOLDS "taaaaaaaaaactaattaccactctctgaaacgcgttagagagtaacgaggggttaggtaa\n";
print SCAFFOLDS "tactgatagatagagaaaaatggctactttttcaaacctttctattcacatatataatcat\n";
print SCAFFOLDS "tcatacaataccaaaaaaataaagtctcataaccaaaagcgacatgtttctagccggacaa\n";
print SCAFFOLDS "ttcaagagatttgagagggttattccggtgccttggtcccagctacgaattcattcatata\n";
print SCAFFOLDS "tgctcacacgaaatgtgaacgagtgaggggtgagagcatgacagaaaattgaagttaagag\n";
print SCAFFOLDS "tgtttaattggaacgtaccttgactttaccgtccgtcgagttctccatatgattaatcaat\n";
print SCAFFOLDS "tgatggagcgctggtagcaagcattatattgttttgtgtttgatgagctgaaaattttttg\n";
print SCAFFOLDS "tataactttttgaaaaaaaaaagttcataatctgaaggtactgaaactgccaatacttcca\n";
print SCAFFOLDS "tgttaaagacgaaaacatgcaggttgagtgagatagaaggagtgaacggcaagtttccttt\n";
print SCAFFOLDS "tcacatttcttctaacataacgtatctatgtcagatctaaaactagtgttacgcgcgacac\n";
print SCAFFOLDS "tagatctcatattgataagcacccggttaccatgttattcgcggctacggttgggaaagga\n";
print SCAFFOLDS "ttagagttggaaaaatgggtaagactactttttcgaaatttgtcaaatgtgtcttatatct\n";
print SCAFFOLDS "ttcttgctcattaagagccccccttctatccccgtcgtttcatcaagtaatcatttgtttc\n";
print SCAFFOLDS "ttggtgtgcttgccgagtagtggcaacatttcggaatgggttttcttgttctcacatgtcc\n";
print SCAFFOLDS "tcattgatccgcttctattgttatttgctacccatcttgaatataaaagtcccttcatcct\n";
print SCAFFOLDS "tattcttagaaagtttttggctaatagtactgaagagaacggtggtgacccacacacacac\n";
print SCAFFOLDS "taagttgaacaaacatcgcggcagggggaaataagtatttggaaaaaaaatggagtgttgt\n";
print SCAFFOLDS "taagacgctgatgagaggagcaagcaaacgaacaaataatgctaacattgagaacagtgaa\n";
print SCAFFOLDS "taaaagatcgaaagactggatggcaactattcaaattaactggtagatagttccccccccc\n";
print SCAFFOLDS "tcccccccccgaaacgaagtgaaacgtaacgaaatcatcgacgagtagagtatatagaata\n";
print SCAFFOLDS "ttgagacaattacagtgaagtacagcggaggcagaaagggtgcgaacgacgcggcacgagg\n";
print SCAFFOLDS "ttggattgggattatgcaggtcaggtcctcaatgaaatgacggtactttacttgcaaagac\n";
print SCAFFOLDS "tcatttcgaattagcggtcgtactggtagtagtggtcagaagatcgtaaccctacactttt\n";
print SCAFFOLDS "tgcctatttttgggggggggggggagcaagatgattagcacaattactcaactcataatct\n";
print SCAFFOLDS "ttactagggaatgtgtaacctattgagtggtgttatagaatgaatgtagtgtcattggata\n";
print SCAFFOLDS "tgctgattttatgttcttctcgaatataagaaaattattttccggcggatatctagaactt\n";
print SCAFFOLDS "tctatttgcgtccaaagtttagagccgatttcagttgattttctagagtatttcaaaatta\n";
print SCAFFOLDS "tttgcgggaaatcatagtattaaactggctcaataatttttcataagcttgctaaattggg\n";
print SCAFFOLDS "taaaagtcattagtatctttagcaaaaactagcatttcggaaatatcatttgcctttatat\n";
print SCAFFOLDS "tccggagtatacacttccggagcacaaaaaaaaatgcgagaatgatacttattacattacc\n";
print SCAFFOLDS "tagggtatccaagagtgataaaaagttaatctagtggttacaacatttactagcttccaaa\n";
print SCAFFOLDS "taagttttttaaataaacttc\n";
close(SCAFFOLDS);
# RUN FORMATDB ON THE SCAFFOLDS:
system "formatdb -i $scaffolds -p F -o T";
($BLAST,$errorcode,$errormsg) = &check_for_blast_match2($seqfile,$scaffolds,$outputdir,0.05,25000);
if ($errorcode != 0) { print STDERR "ERROR: test_check_for_blast_match2: failed test1\n"; exit;}
# OUTPUT FILE SHOULD HAVE:
# FOX-1 X:2445317..2450316 42.59 216 97 6 221 409 1404 784 1e-34 96.7
# FOX-1 X:2445317..2450316 54.84 62 8 2 180 221 1585 1403 1e-34 56.6
# FOX-1 X:2445317..2450316 50.82 61 11 2 160 201 1646 1464 4e-10 50.4
# FOX-1 X:2445317..2450316 100.00 14 0 0 68 81 3535 3494 6e-08 32.7
# FOX-1 X:2445317..2450316 92.86 14 1 0 55 68 3575 3534 6e-08 29.6
# FOX-1 X:2445317..2450316 42.19 64 33 2 356 415 947 765 3e-07 41.2
# FOX-1 X:2445317..2450316 80.00 10 2 0 80 89 2255 2226 0.045 23.9
# COLUMNS ARE (FROM http://bergmanlab.smith.man.ac.uk/?p=41):
# queryId, subjectId, percIdentity, alnLength, mismatchCount, gapOpenCount, queryStart, queryEnd, subjectStart, subjectEnd, eVal, bitScore
# SHOULD GET 765-3575, THEN TAKE 25000 ON EITHER SIDE SO -24235 TO 28575
if ($BLAST->{"X:2445317..2450316"} ne '-24235=28575') { print STDERR "ERROR: test_check_for_blast_match2: failed test1\n"; exit;}
system "rm -f $seqfile";
system "rm -f $scaffolds";
# DELETE THE FORMATDB OUTPUT FILES:
$formatdbfile = $scaffolds.".nhr";
system "rm -f $formatdbfile";
$formatdbfile = $scaffolds.".nin";
system "rm -f $formatdbfile";
$formatdbfile = $scaffolds.".nsd";
system "rm -f $formatdbfile";
$formatdbfile = $scaffolds.".nsi";
system "rm -f $formatdbfile";
$formatdbfile = $scaffolds.".nsq";
system "rm -f $formatdbfile";
}
#------------------------------------------------------------------#
# CHECK IF THERE IS A BLAST MATCH BETWEEN A TREEFAM FAMILY AND THE FILE OF SCAFFOLDS:
sub check_for_blast_match2
{
my $seqfile = $_[0]; ## FILE WITH PROTEIN SEQUENCES FOR A TREEFAM FAMILY
my $scaffolds = $_[1]; ## FILE WITH DNA SEQUENCE FOR THE SCAFFOLDS
my $outputdir = $_[2]; ## DIRECTORY TO WRITE OUTPUT FILES TO
my $evalue_cutoff = $_[3]; ## EVALUE CUTOFF TO USE FOR BLAST MATCHES
my $flank_length = $_[4]; ## LENGTH OF SEQUENCE TO TAKE ON EITHER SIDE OF A BLAST MATCH
my $random_number; ## RANDOM NUMBER FOR TEMPORARY FILE NAME
my $output; ## THE BLAST OUTPUT FILE
my @temp; ##
my $line; ##
my $evalue; ## EVALUE FOR THE BLAST MATCH
my $errorcode = 0; ## RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";## RETURNED AS 'none' IF THERE IS NO ERROR
my $scaffold; ## NAME OF SCAFFOLD WITH THE BLAST MATCH
my $scaffold_start; ## START OF BLAST MATCH IN SCAFFOLD
my $scaffold_end; ## END OF BLAST MATCH IN SCAFFOLD
my %BLAST = (); ## RECORD POSITIONS OF BLAST MATCHES FOR EACH SCAFFOLD
my $pos; ## POSITION OF BLAST MATCH IN A SCAFFOLD
my $hits; ## POSITIONS OF HITS IN A SCAFFOLD
my $new_hits; ## POSITIONS OF HITS IN A SCAFFOLD, AFTER MERGING OVERLAPPING HITS
my %SEEN = (); ## HASH TABLE TO RECORD WHETHER WE ALREADY SAW A PARTICULAR HIT POSITION
my $tmp; ##
# RUN BLAST BETWEEN THE PROTEIN SEQUENCES AND THE SCAFFOLDS:
# -m 8 GIVES TABULAR BLAST FORMAT
$random_number = rand();
$output = $outputdir."/tmp".$random_number;
system "blastall -p tblastn -i $seqfile -d $scaffolds -o $output -m 8\n";
sleep(1);
# READ IN THE BLAST OUTPUT AND SEE IF THERE ARE ANY MATCHES:
open(OUTPUT,"$output") || die "ERROR: check_for_blast_match2: cannot open $output\n";
while(<OUTPUT>)
{
$line = $_;
chomp $line;
@temp = split(/\s+/,$line);
# COLUMNS ARE (FROM http://bergmanlab.smith.man.ac.uk/?p=41):
# queryId, subjectId, percIdentity, alnLength, mismatchCount, gapOpenCount, queryStart, queryEnd, subjectStart, subjectEnd, eVal, bitScore
$scaffold = $temp[1];
$scaffold_start = $temp[8];
$scaffold_end = $temp[9];
if ($scaffold_end < $scaffold_start)
{
$tmp = $scaffold_end;
$scaffold_end = $scaffold_start;
$scaffold_start = $tmp;
}
$evalue = $temp[10];
if ($evalue <= $evalue_cutoff)
{
# TAKE 25 kb ON EITHER SIDE OF THE BLAST MATCH:
$scaffold_start = $scaffold_start - $flank_length;
$scaffold_end = $scaffold_end + $flank_length;
$pos = $scaffold_start."=".$scaffold_end;
if (!($SEEN{$scaffold."=".$pos}))
{
if (!($BLAST{$scaffold})) { $BLAST{$scaffold} = $pos; }
else { $BLAST{$scaffold} = $BLAST{$scaffold}.",".$pos;}
$SEEN{$scaffold."=".$pos} = 1;
}
}
}
close(OUTPUT);
# MERGE ANY OVERLAPPING HITS IN ANY SCAFFOLD:
foreach $scaffold (keys %BLAST)
{
$hits = $BLAST{$scaffold};
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0) { ($errorcode,$errormsg) = &print_error($errormsg,$errorcode,0); }
$BLAST{$scaffold} = $new_hits;
}
# DELETE TEMPORARY FILES:
system "rm -f $output";
return(\%BLAST,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &merge_overlapping_hits
sub test_merge_overlapping_hits
{
my $hits; # POSITIONS OF HITS IN SCAFFOLD
my $new_hits; # POSITIONS OF MERGED HITS IN SCAFFOLD
my $errorcode; # RETURNED AS 0 BY A FUNCTION IF THERE IS NO ERROR
my $errormsg; # RETURNED AS 'none' BY A FUNCTION IF THERE IS NO ERROR
$hits = "20=40,35=50";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0 || $new_hits ne '20=50') { print STDERR "ERROR: test_merge_overlapping_hits: failed test1\n"; exit;}
$hits = "35=50,20=40";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0 || $new_hits ne '20=50') { print STDERR "ERROR: test_merge_overlapping_hits: failed test2\n"; exit;}
$hits = "20=40,35=50,45=60";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0 || $new_hits ne '20=60') { print STDERR "ERROR: test_merge_overlapping_hits: failed test3\n"; exit;}
$hits = "20=40,120=150,35=50";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0 || $new_hits ne '20=50,120=150') { print STDERR "ERROR: test_merge_overlapping_hits: failed test4 (hits $hits new_hits $new_hits)\n"; exit;}
$hits = "20=40";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 0 || $new_hits ne '20=40') { print STDERR "ERROR: test_merge_overlapping_hits: failed test5\n"; exit;}
$hits = "20=40,20=40";
($new_hits,$errorcode,$errormsg) = &merge_overlapping_hits($hits);
if ($errorcode != 14) { print STDERR "ERROR: test_merge_overlapping_hits: failed test6\n"; exit;}
}
#------------------------------------------------------------------#
# MERGE ANY OVERLAPPING HITS IN A SCAFFOLD:
sub merge_overlapping_hits
{
my $hits = $_[0]; # POSITIONS OF HITS IN THE SCAFFOLD
my @hits; # ARRAY OF THE POSITIONS OF HITS IN THE SCAFFOLD
my $no_hits; # NUMBER OF HITS IN THE SCAFFOLD
my $i; #
my $hit; # A HIT IN THE SCAFFOLD
my $errorcode = 0; # RETURNED AS 0 IF THERE IS NO ERROR
my $errormsg = "none";# RETURNED AS 'none' IF THERE IS NO ERROR
my $hit_start; # START OF $hit
my $hit_end; # END OF $hit
my @temp; #
my $prev_hit = "none";# THE PREVIOUS HIT
my $prev_hit_start; # START OF THE PREVIOUS HIT
my $prev_hit_end; # END OF THE PREVIOUS HIT
my $new_hits = ""; # THE FINAL MERGED HITS
my %START = (); # HASH TABLE WITH THE START POSITIONS OF HITS
# SORT THE HITS BY START-POINT:
@hits = split(/\,/,$hits);
$no_hits = $#hits + 1;
for ($i = 1; $i <= $no_hits; $i++)
{
$hit = $hits[($i-1)];
@temp = split(/=/,$hit);
$hit_start = $temp[0];
if ($START{$hit})
{
$errormsg = "ERROR: merge_overlapping_hits: already have start for $hit\n";
$errorcode = 14; # ERRORCODE=14
return($new_hits,$errorcode,$errormsg);
}
$START{$hit} = $hit_start;
}
@hits = reverse sort { $START{$b} <=> $START{$a} } keys %START;
# MERGE OVERLAPPING HITS:
for ($i = 1; $i <= $no_hits; $i++)
{
$hit = $hits[($i-1)];
@temp = split(/=/,$hit);
$hit_start = $temp[0];
$hit_end = $temp[1];
# CHECK IF THIS HIT OVERLAPS WITH THE PREVIOUS HIT:
if ($i > 1)
{
if (($hit_start >= $prev_hit_start && $hit_start <= $prev_hit_end) ||
($hit_end >= $prev_hit_start && $hit_end <= $prev_hit_end) ||
($prev_hit_start >= $hit_start && $prev_hit_start <= $hit_end) ||
($prev_hit_end >= $hit_start && $prev_hit_end <= $hit_end))
{
# THIS HIT OVERLAPS WITH THE PREVIOUS HIT, SO MERGE THEM:
if ($hit_start < $prev_hit_start)
{
$errormsg = "ERROR: hit_start $hit_start prev_hit_start $prev_hit_start (hits $hits)\n";
$errorcode = 10; # ERRORCODE=10
return($new_hits,$errorcode,$errormsg);
}
if ($hit_end > $prev_hit_end) { $prev_hit_end = $hit_end;}
}
else # THIS HIT DOES NOT OVERLAP WITH THE PREVIOUS HIT, SO RECORD THE PREVIOUS HIT:
{
$prev_hit = $prev_hit_start."=".$prev_hit_end;
$new_hits = $new_hits.",".$prev_hit;
$prev_hit_start= $hit_start;
$prev_hit_end = $hit_end;
}
}
elsif ($i == 1)
{
$prev_hit_start = $hit_start;
$prev_hit_end = $hit_end;
}
}
$prev_hit = $prev_hit_start."=".$prev_hit_end;
$new_hits = $new_hits.",".$prev_hit;
if ($new_hits eq '')
{
$errormsg = "ERROR: merge_overlapping_hits: new_hits $new_hits\n";
$errorcode = 15; # ERRORCODE=15
return($new_hits,$errorcode,$errormsg);
}
$new_hits = substr($new_hits,1,length($new_hits)-1);
return($new_hits,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# READ IN ONE HMM AT A TIME FROM THE INPUT FILE OF HMMs:
sub run_genewise_on_hmm
{
my $hmmfile = $_[0]; ## THE HMM FILE
my $outputdir = $_[1]; ## DIRECTORY TO WRITE OUTPUT FILES TO
my $input_fasta = $_[2]; ## INPUT FASTA FILE
my $hmmname = $_[3]; ## NAME OF THE HMM
my $spliceflat = $_[4]; ## SAYS WHETHER WE WANT TO USE THE -splice flat OPTION IN GENEWISE
my $parameterfile = $_[5]; ## THE NAME OF THE INTRON PARAMETER FILE
my $scaffoldname = $_[6]; ## NAME OF THE SCAFFOLD
my $testing = $_[7]; ## SAYS WHETHER THIS FUNCTION IS BEING CALLED BY A TESTING SUBROUTINE
my $errorcode = 0; ## RETURNED AS 0 IF THERE IS NO ERROR.
my $errormsg = "none";## RETURNED AS 'none' IF THERE IS NO ERROR.
my $random_number; ## RANDOM NUMBER FOR TEMPORARY FILE NAME
my $genewise = "none";## TEMPORARY FILE FOR THE GENEWISE OUTPUT FOR THIS HMM
my $cmd; ## THE GENEWISE COMMAND TO RUN
my $genewise_exe = "/nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/src/bin/genewise"; # wise 2.4.1, can use -genestats
# CHECK THE SPLICEFLAT OPTION IS 'yes' OR 'no':
if ($spliceflat ne 'yes' && $spliceflat ne 'no')
{
$errormsg = "ERROR: run_genewise_on_hmm: spliceflat is $spliceflat (should be yes or no)\n";
$errorcode = 4; # ERRORCODE=4
return($genewise,$errorcode,$errormsg);
}
# RUN GENEWISE:
$random_number = rand();
$genewise = $outputdir."/tmp".$random_number;
if ($testing == 0) { print STDERR "___ scaffold $scaffoldname: running genewise for HMM $hmmname ...\n";}
if ($parameterfile ne 'none' && $spliceflat eq 'no')
{
$cmd = "$genewise_exe $hmmfile $input_fasta -gff -hmmer -genestats $parameterfile -nosplice_gtag -quiet -erroroffstd > $genewise";
}
elsif ($parameterfile eq 'none' && $spliceflat eq 'yes')
{
$cmd = "$genewise_exe $hmmfile $input_fasta -gff -hmmer -splice_gtag -quiet -erroroffstd > $genewise";
}
elsif ($parameterfile eq 'none' && $spliceflat eq 'no')
{
$cmd = "$genewise_exe $hmmfile $input_fasta -gff -hmmer -nosplice_gtag -quiet -erroroffstd > $genewise"; # USES THE gene.stats FILE THAT COMES WITH GENEWISE, /nfs/users/nfs_a/alc/Documents/GeneWise/wise2.4.1/wisecfg/gene.stat
}
elsif ($parameterfile ne 'none' && $spliceflat eq 'yes')
{
$errormsg = "ERROR: run_genewise_on_hmm: cannot have spliceflat $spliceflat and parameterfile $parameterfile\n";
$errorcode = 5; # ERRORCODE=5
return($genewise,$errorcode,$errormsg);
}
system "$cmd";
sleep(1);
return($genewise,$errorcode,$errormsg);
}
#------------------------------------------------------------------#
# TEST &print_error
sub test_print_error
{
my $errormsg; # RETURNED AS 'none' FROM A FUNCTION IF THERE WAS NO ERROR
my $errorcode; # RETURNED AS 0 FROM A FUNCTION IF THERE WAS NO ERROR
($errormsg,$errorcode) = &print_error(45,45,1);
if ($errorcode != 12) { print STDERR "ERROR: test_print_error: failed test1\n"; exit;}
($errormsg,$errorcode) = &print_error('My error message','My error message',1);
if ($errorcode != 11) { print STDERR "ERROR: test_print_error: failed test2\n"; exit;}
($errormsg,$errorcode) = &print_error('none',45,1);
if ($errorcode != 13) { print STDERR "ERROR: test_print_error: failed test3\n"; exit;}
($errormsg,$errorcode) = &print_error('My error message', 0, 1);
if ($errorcode != 13) { print STDERR "ERROR: test_print_error: failed test4\n"; exit;}
}
#------------------------------------------------------------------#
# PRINT OUT AN ERROR MESSAGE AND EXIT.
sub print_error
{
my $errormsg = $_[0]; # THIS SHOULD BE NOT 'none' IF AN ERROR OCCURRED.
my $errorcode = $_[1]; # THIS SHOULD NOT BE 0 IF AN ERROR OCCURRED.
my $called_from_test = $_[2]; # SAYS WHETHER THIS WAS CALLED FROM test_print_error OR NOT
if ($errorcode =~ /[A-Z]/ || $errorcode =~ /[a-z]/)
{
if ($called_from_test == 1)
{
$errorcode = 11; $errormsg = "ERROR: print_error: the errorcode is $errorcode, should be a number.\n"; # ERRORCODE=11
return($errormsg,$errorcode);
}
else
{
print STDERR "ERROR: print_error: the errorcode is $errorcode, should be a number.\n";
exit;
}
}
if (!($errormsg =~ /[A-Z]/ || $errormsg =~ /[a-z]/))
{
if ($called_from_test == 1)
{
$errorcode = 12; $errormsg = "ERROR: print_error: the errormessage $errormsg does not seem to contain text.\n"; # ERRORCODE=12
return($errormsg,$errorcode);
}
else
{
print STDERR "ERROR: print_error: the errormessage $errormsg does not seem to contain text.\n";
exit;
}
}
if ($errormsg eq 'none' || $errorcode == 0)
{
if ($called_from_test == 1)
{
$errorcode = 13; $errormsg = "ERROR: print_error: errormsg $errormsg, errorcode $errorcode.\n"; # ERRORCODE=13
return($errormsg,$errorcode);
}
else
{
print STDERR "ERROR: print_error: errormsg $errormsg, errorcode $errorcode.\n";
exit;
}
}
else
{
print STDERR "$errormsg";
exit;
}
return($errormsg,$errorcode);
}
#------------------------------------------------------------------#
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