from datetime import datetime start_time = "10:44:09.339931" end_time = "10:44:09.376233" t1 = datetime.strptime(start_time, "%H:%M:%S.%f") print('Start time:', t1.time()) t2 = datetime.strptime(end_time, "%H:%M:%S.%f") print('End time:', t2.time()) delta = t2 - t1 ms = delta.total_seconds() * 1000 print(f"Time difference is {ms} milliseconds")