Manual page here: http://software.broadinstitute.org/software/igv/batch These commands are also available: http://software.broadinstitute.org/software/igv/PortCommands Download here: http://software.broadinstitute.org/software/igv/download After installing IGV, a script can be run from the terminal. Sample script (save this as `IGV_test.bat`): ``` new snapshotDirectory /path/to/snapshot_dir load /path/to/sample1.bam load /path/to/sample2.bam genome hg19 maxPanelHeight 400 goto chr11:108138003-108138003 snapshot custom_chr11-108138003-108138003.png goto chr9:21971111-21971111 snapshot custom_chr9-21971111-21971111.png exit ``` Example command to run IGV: ``` bin/IGV_2.3.81/igv.sh -b code/IGV_test.bat ``` Running the script like this will cause a window to appear, and the commands will be executed. If you are running IGV from a headless server (accessing the server via `ssh`), then this will require X11 to be running on the server and installed on your local computer (use XQuartz for OS X). Remember to enable X11 in your terminal by adding `-Y` to your `ssh` command, like so: ``` ssh username@server.edu -Y ``` For scripting purposes, you can run the X11 virtualizer to avoid the requirement for an active X11 window when you call IGV. Use this command: ``` (Xvfb :10 &) && DISPLAY=:10 java -Xmx750m -jar bin/IGV_2.3.81/igv.jar -b code/IGV_test.bat && killall Xvfb ``` Example output: ![chr3_185850354-185872709](https://cloud.githubusercontent.com/assets/10505524/19937792/013ef1d8-a0f9-11e6-8b17-289de9706c1d.png)