The latest beta (3.5) includes separate color settings for light & dark mode. Toggling dark mode automatically switches colors.
Vist iTerm2 homepage or use brew install iterm2-beta to download the beta. Thanks @stefanwascoding.
- Add switch_automatic.pyto~/Library/ApplicationSupport/iTerm2/Scripts/AutoLaunchwith:
#!/usr/bin/env python3
import asyncio
import iterm2
async def main(connection):
    async with iterm2.VariableMonitor(connection, iterm2.VariableScopes.APP, "effectiveTheme", None) as mon:
        while True:
            # Block until theme changes
            theme = await mon.async_get()
            # Themes have space-delimited attributes, one of which will be light or dark.
            parts = theme.split(" ")
            if "dark" in parts:
                preset = await iterm2.ColorPreset.async_get(connection, "Dark Background")
            else:
                preset = await iterm2.ColorPreset.async_get(connection, "Light Background")
            # Update the list of all profiles and iterate over them.
            profiles=await iterm2.PartialProfile.async_query(connection)
            for partial in profiles:
                # Fetch the full profile and then set the color preset in it.
                profile = await partial.async_get_full_profile()
                await profile.async_set_color_preset(preset)
iterm2.run_forever(main)Change Dark Background and Light Background to color presets you like.
- Enabled switch_automatic.pyfrom Scripts menu.


Probably the same script already shared here https://gist.github.com/jamesmacfie/2061023e5365e8b6bfbbc20792ac90f8#file-auto_dark_mode-py